Interferon as adjuvant therapy with initial radiotherapy of patients with anaplastic gliomas

Ms, Mahaley; Mb, Urso; Ra, Whaley; Te, Williams; Guaspari, A

doi:10.3171/jns.1984.61.6.1069

Cited by 22 publications

(4 citation statements)

References 7 publications

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“…In the presence of increased levels of INF-p, IFN-y and TNF-CY, appropriately programmed cells might express both ICAM-1 and Class I1 HLA antigens (Maio et al, 1989a;Frohman et al, 1989), which are required in combination with each other and an appropriate T-cell receptor to activate T lymphocytes (Altmann et al, 1989). These immunologic reactions, as well as a direct anti-proliferative effect, may in part account for the anti-tumor activity demonstrated in certain patients with CNS tumors who are exposed to recombinant interferons (Nagai and Arai, 1984;Mahaley et al, 1984Mahaley et al, , 1985Bogdahn et al, 1985;Duffer al., 1986;Fete11 et al, 1990).…”

Section: Resultsmentioning

confidence: 99%

Expression and modulation by cytokines of the intercellular adhesion molecule‐1 (ICAM‐1) in human central nervous system tumor cell cultures

Guarini

Temponi

Bruce

et al. 1990

Intl Journal of Cancer

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The intercellular adhesion molecule (ICAM-1) has been shown to be important in interactions involving cells of the immune system and to be upregulated in a number of cell culture systems by cytokines, including immune interferon (IFN-gamma) and tumor necrosis factor-alpha (TNF-alpha). In the present study, we have determined by fluorescence-activated cell sorter (FACS) analysis and the anti-ICAM-1 monoclonal antibody (MAb) CL203.4 the base-line expression of ICAM-1 and its modulation by recombinant IFN-beta, IFN-gamma and TNF-alpha in early passage (less than 15) human central nervous system (CNS) tumor-derived cell cultures. These cultures were established from various malignancies, including glioblastoma multiforme (GBM), astrocytoma (AST), ganglioglioma, medulloblastoma, meningioma and a pineal tumor. ICAM-1 expression was highest in the GBM- and AST-derived cell cultures and was lowest in the ganglioglioma and normal pineal cell cultures. Variable ICAM-1 expression was found, however, in tumors of the same histological group. In several cell cultures the variable expression observed by FACS was substantiated by the intensity of the molecular species immunoprecipitated by the anti-ICAM-1 MAb CL203.4 from these cells. All the cell cultures displayed variable but consistent increases in ICAM-1 expression following treatment with IFN-gamma or TNF-alpha. In general, the degree of increase in ICAM-1 expression was greatest in cultures exposed to TNF-alpha. Upregulation of ICAM-1 expression in an established glioblastoma multiforme cell line was of greater magnitude and more rapid following TNF-alpha treatment (within 2 to 3 hr) than exposure to IFN-gamma (by 24 hr). In several cultures, IFN-beta also increased ICAM-1 expression and enhanced the increase induced by TNF-alpha. The results of the present study indicate that variable expression of ICAM-1 is a common property of early passage cultures of CNS tumors and recombinant interferons and TNF-alpha can differentially upregulate ICAM-1 expression in these CNS tumor cell cultures.

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Section: Resultsmentioning

confidence: 99%

Expression and modulation by cytokines of the intercellular adhesion molecule‐1 (ICAM‐1) in human central nervous system tumor cell cultures

Guarini

Temponi

Bruce

et al. 1990

Intl Journal of Cancer

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“…New techniques of administration may improve the effectiveness of radiation therapy, 52,61,81–83 and two lines of research are being pursued in the chemotherapy of brain tumors: development of new drugs, 84–88 and more effective treatment delivery 89–93 . In addition, biological therapy, with the interferons and with interleukin 2, may be active in primary brain tumors 94–98 …”

Section: Principles Of Managementmentioning

confidence: 99%

Primary Brain Tumors in the Older Patient: An Annotated Review

Zachariah

Wang

et al. 1992

J American Geriatrics Society

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“…Although Interferon (IFN) has been reported to have antiproliferative activities against malignant brain tumors [1,2] and other types of malignancies, the molecular basis of the antiproliferative activity of IFN is still uncertain. It is especially important to determine the effect of IFN on cell cycle phase perturbation and obtain an effective protocol for IFN dosimetry and further trials of combination chemotherapy [3 -6].…”

Section: Introductionmentioning

confidence: 99%

Effect of interferon-� on the cell cycle of human glioma cell line U-251 MG: flow cytometric two-dimensional (BrdU/DNA) analysis

et al. 1988

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This paper describes the determination of the effect of IFN-beta on U-251 MG cells using the bromodeoxyuridine (BrdU/DNA) analysis technique. The cell cycle perturbation of exponentially growing cells was estimated by a newly developed two-dimensional analysis of sequential BrdU/DNA distributions measured at 4-hr intervals after IFN-beta administration. The U-251 MG cell line was sensitive to IFN-beta, and cell proliferation was inhibited by 50.0% at 48 hr. Analysis of DNA histograms indicated that IFN-beta accumulated the cells in the S-phase, from 16 to 48 hr after treatment. In the two-dimensional analysis, labeled cells treated with IFN-beta moved from the S-phase through the G2M-phase and then entered the G1-phase within 12 hr after the initial treatment, in a pattern similar to labeled cells untreated with IFN-beta. After 16 hr, labeled cells treated with IFN-beta began to accumulate in the S-phase and remained there even after 48 hr. These results imply that IFN-beta may have an effect on the G1-phase, thereby inducing S-phase accumulation of human glioma cell line U-251 MG.

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Interferon as adjuvant therapy with initial radiotherapy of patients with anaplastic gliomas

Abstract: In a group of nine patients with anaplastic gliomas, survival following surgery and treatment with interferon and radiotherapy was comparable to survival for a matched group of patients treated with BCNU and radiotherapy following surgery.

Cited by 22 publications

References 7 publications

Expression and modulation by cytokines of the intercellular adhesion molecule‐1 (ICAM‐1) in human central nervous system tumor cell cultures

Expression and modulation by cytokines of the intercellular adhesion molecule‐1 (ICAM‐1) in human central nervous system tumor cell cultures

Primary Brain Tumors in the Older Patient: An Annotated Review

Effect of interferon-� on the cell cycle of human glioma cell line U-251 MG: flow cytometric two-dimensional (BrdU/DNA) analysis

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