Interferon Stimulated Gene 15 and Myxovirus Resistance 2 Genes are Upregulated during Early Pregnancy in Buffaloes

Nag, B.S. Pradeep; Arunmozhi, N; Sarath, T.; Asokan, S.; Vijayarani, K.; Gomaraschi, Monica; Gopikrishnan, D.; Krishnakumar, Karishma

doi:10.20546/ijcmas.2018.712.204

Cited by 6 publications

(3 citation statements)

References 16 publications

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“…These results are in line with the observations by Thakur et al [36] and Mishra and Sarkar [58], who reported that the gene expression profiles of OAS1, MX2 and ISG15 in buffalo PBMCs increased between days 14 and 20 of pregnancy and declined thereafter. Our findings are also in agreement with the results reported by Nag et al [37], which showed a significant increase in the expression of ISG15 in buffalo PBMCs on days 18 and 20 and of MX1 on days 14e20 of pregnancy. Recently, Huidrom et al [28] investigating the effect in vitro of pre-implantation embryo on the ISGs Fig.…”

Section: Discussionsupporting

confidence: 94%

Gene Expression of Pregnancy-Associated Glycoproteins-1 (PAG-1), Interferon-Tau (IFNt) and Interferon Stimulated Genes (ISGs) as Diagnostic and Prognostic Markers of Maternal-Fetal Cellular Interaction in Buffalo Cows

Casano

Menchetti

Marinucci

et al. 2023

Preprint

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Section: Discussionsupporting

confidence: 94%

Gene Expression of Pregnancy-Associated Glycoproteins-1 (PAG-1), Interferon-Tau (IFNt) and Interferon Stimulated Genes (ISGs) as Diagnostic and Prognostic Markers of Maternal-Fetal Cellular Interaction in Buffalo Cows

Casano

Menchetti

Marinucci

et al. 2023

Preprint

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“…In a previous work, we found that in buffalo, too, the ISGs expression proved to be a reliable peripheral biomarker for the prediction of pregnancy and embryo mortality during the peri-implantation period, with respect to IFNt expression. The ISGs were more strongly expressed in the pregnant than in the non-pregnant animals, in line with the observations reported by other authors [12,51,52].…”

Section: Discussionsupporting

confidence: 92%

Interferon-Tau in Maternal Peripheral Blood and Its Relationship with Progesterone and Pregnancy-Associated Glycoproteins in the Early Phases of Gestation in Water Buffalo

Barbato,

Menchetti,

Casano

et al. 2024

Animals

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The aim of this study was to investigate the interferon tau (IFNt) concentration in the peripheral maternal blood during the early phase of pregnancy in buffalo cows and improve the knowledge on the physiological importance of circulating IFNt, evaluating the possible interaction with pregnancy-associated glycoproteins (PAGs) and progesterone (P4). Blood samples were taken from buffalo cows on day 0 (day of AI), 7, 14, 18, 28, and 40 post insemination for the IFNt, PAG, and P4 analysis and to determine the IFNt mRNA expression. The animals were categorized ex post into Pregnant, Non-pregnant and Embryo mortality groups. The interferon value was influenced by group (p = 0.003), being always higher in pregnant buffalo cows than in non-pregnant ones, while the embryo mortality group showed intermediate values between those for pregnant and non-pregnant animals. The mRNA expression of IFNt was not influenced by groups or any time points. The regression analysis that included IFNt as the independent variable showed that PAGs, from day 18 (p < 0.01), and P4, from day 28 (p < 0.05), were positively associated with IFNt values. The close associations among IFNt, PAGs and P4 demonstrate that all three molecules work together for fetal–placental well-being and pregnancy support. Unfortunately, the great individual variability in circulating IFNt makes this analysis unsuitable for early pregnancy diagnosis.

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“…Its role in endometrial receptivity and embryo survivability is well recognized. Extra uterine ISG15 expression in CL and peripheral blood leukocytes (PBL) of bovine (Yang et al 2010, Cheng et al 2019, bubaline (Jain et al 2012, Nag et al 2018 and ovine (Oliveira et al 2008) had also been reported. Findings of the current study are comparable with other studies in bovine (Yang et al 2010), bubaline (Jain and Mitra 2012) and ewes (Chen et al 2006, Oliveira et al 2008) in which ISG15 level was upregulated in CL during early stage of pregnancy (13 to 24 days).…”

Section: Resultsmentioning

confidence: 97%

Interferon-stimulated gene 15 (ISG15): Expression profile and role in the corpus luteum of goat (Capra hircus)

Jain

Chandrakar

et al. 2022

Indian J of Anim Sci

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The corpus luteum (CL) is a dynamic and transient endocrine gland. It secretes variety of protein hormonesand have an important role in survivability of the embryo during early pregnancy. Among the interferon stimulatedgenes (ISGs), interferon-stimulated gene 15 (ISG15) is one of the gene having important role in maintaining corpusluteum in different ruminant species, but in goat (Capra hircus) its role is not yet elucidated. In the present study,temporal expression profile of cpISG15 mRNA and protein was examined by quantitative real-time PCR (qPCR)and western blot techniques, respectively, in the CL of cyclic and pregnant does. Both cpISG15 mRNA and proteinwere expressed maximally in the CL during early stage of pregnancy (16 to 24 days) as compared to cyclic does.But significant difference was not found in the expression of cpISG15 mRNA and protein in the CL between thelater stage of pregnancy (25 to 40 days) and cyclic does. Finding of significant upregulation of ISG15 in CL duringearly pregnancy at mRNA as well as protein level suggest its probable role in maintaining CL and its function at thisstage in caprine.

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Interferon Stimulated Gene 15 and Myxovirus Resistance 2 Genes are Upregulated during Early Pregnancy in Buffaloes

Cited by 6 publications

References 16 publications

Gene Expression of Pregnancy-Associated Glycoproteins-1 (PAG-1), Interferon-Tau (IFNt) and Interferon Stimulated Genes (ISGs) as Diagnostic and Prognostic Markers of Maternal-Fetal Cellular Interaction in Buffalo Cows

Gene Expression of Pregnancy-Associated Glycoproteins-1 (PAG-1), Interferon-Tau (IFNt) and Interferon Stimulated Genes (ISGs) as Diagnostic and Prognostic Markers of Maternal-Fetal Cellular Interaction in Buffalo Cows

Interferon-Tau in Maternal Peripheral Blood and Its Relationship with Progesterone and Pregnancy-Associated Glycoproteins in the Early Phases of Gestation in Water Buffalo

Interferon-stimulated gene 15 (ISG15): Expression profile and role in the corpus luteum of goat (Capra hircus)

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