Interferon stimulated genes as peripheral diagnostic markers of early pregnancy in sheep: a critical assessment

Mauffré, Vincent; Grimard, Bénédicte; Eozénou, Caroline; Inghels, S.; Silva, Luiza Eduarda da; Giraud-Delville, C.; Capo, Damien; Sandra, Olivier; Constant, Fabienne

doi:10.1017/s175173111600077x

Cited by 20 publications

(24 citation statements)

References 33 publications

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“…2). These findings were similar to previous results that there were changed expressions of ISG15 in endometrium [3,24] , blood cells [25,26] , CL [6,27] during early pregnancy in sheep and cattle. It was indicated that the embryonic signal (IFNT) reached the blood cells, CL and BM to up-regulate ISG15 expression through blood or/and immune cells during early pregnancy in sheep.…”

Section: Discussionsupporting

confidence: 92%

Koyunların Erken Gebelik Döneminde Kemik iliğinde ISG15 Ekspresyonu

Yang¹,

Liu²,

Yan³

et al. 2017

Kafkas Univ Vet Fak Derg

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Interferon-tau (IFNT) is the main signal for maternal recognition of pregnancy in ruminants. IFNT acts on the endometrium, corpus luteum, and liver through paracrine and endocrine style, which is involved in inhibiting the development of luteolytic mechanism and suppressing maternal immune rejection of the semi-allogeneic fetus. Bone marrow (BM) is a key component of the lymphatic system, supports the body's immune system through producing the lymphocytes. At present study, the BM was obtained from days 13, 16 and 25 of pregnant ewes, day 16 of non-pregnant ewes to study the expression of interferon stimulated gene 15 kDa protein (ISG15) mRNA and protein through a qRT-PCR assay, Western blot, and an immunohistochemistry analysis. Our results showed that the expression of ISG15 mRNA, proteins and conjugated proteins were up-regulated in the stroma of BM during early pregnancy, and the immunohistochemistry results confirmed that the ISG15 proteins were localized in the cytoplasm of different cells in the stroma of BM. In conclusion, IFNT derived from the conceptus induced upregulated expression of ISG15 and conjugated proteins in the stroma of BM through an endocrine style, which were involved in regulating the maternal immune response during early pregnancy in ewes. Keywords: Ewes, Bone marrow, Pregnancy, Interferon stimulated gene 15 kDa protein Koyunların Erken Gebelik Döneminde Kemik İliğinde ISG15 Ekspresyonu Özetİnterferon-tau (IFNT) ruminantlarda gebeliğin maternal tanısındaki başlıca sinyaldir. IFNT endometrium, korpus luteum ve karaciğer üzerinde parakrin ve endokrin yollarla etki ederek luteolitik mekanizmanın gelişmesini inhibe eder ve semi-allojenik fetusun anne tarafından reddini baskılar. Kemik iliği lenfatik sistemin en önemli bileşenlerinden biri olup lenfositleri üretmek suretiyle vücudun bağışıklık sistemini desteklemektedir. Bu çalışmada, 13, 16 ve 25. günlerde gebe koyunlardan ve 16. günde gebe olmayan koyunlardan kemik iliği alınarak interferon tarafından uyarılan gen 15 kDa protein (ISG15) mRNA ve protein ekspresyonu qRT-PCR, Western blot ve immunohistokimyasal yöntemlerle araştırıldı. Elde edilen sonuçlar ISG15 mRNA, proteinler ile konjuge proteinlerin ekspresyonunun erken gebelik döneminde kemik iliğinde upregule edildiğini ve immunohistokimyasal olarak ISG15 proteinlerin kemik iliğinin stromasında farklı hücrelerin sitoplazmasında yer aldığını göstermiştir. Sonuç olarak, gebeliğe bağlı olarak üretilen IFNT; ISG15 ve konjuge proteinlerin ekspresyonunu kemik iliğinin stromasında endokrin yolla upregüle etmiş ve böylece koyunların erken gebelik döneminde maternal bağışıklık cevabın düzenlenmesinde rol almıştır.

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Section: Discussionsupporting

confidence: 92%

Koyunların Erken Gebelik Döneminde Kemik iliğinde ISG15 Ekspresyonu

Yang¹,

Liu²,

Yan³

et al. 2017

Kafkas Univ Vet Fak Derg

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“…The increase in ISG15 in neutrophils with IFNT supplementation is in agreement with the earlier findings obtained from blood cells in sheep, 16 bone marrow of ewes, 17 and liver biopsies 18 of HF cattle in early pregnancy. The increase in ISG15 in neutrophils with IFNT supplementation is in agreement with the earlier findings obtained from blood cells in sheep, 16 bone marrow of ewes, 17 and liver biopsies 18 of HF cattle in early pregnancy.…”

Section: Discussionsupporting

confidence: 91%

Exploring the concentration‐dependent actions of interferon‐τ on bovine neutrophils to understand the process of implantation

Manjari¹,

Hyder

Kapoor³

et al. 2018

J of Cellular Biochemistry

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Interferon-τ (IFNT) is a major cytokine produced by the ruminant trophoectoderm during the peri-implantation period exerting immunomodulatory actions on various cells including neutrophils. The current in vitro study was undertaken to analyze the concentration-dependent effects of IFNT on neutrophil gene dynamics to understand its possible role in implantation process. The neutrophils were isolated from the blood of heifers and were cultured subjecting them to different IFNT concentrations (1, 5, or 10 ng/mL). The gene-expression patterns of different interferon-stimulated genes, l-selectin, CD31, CD11b, and progesterone-induced blocking factor (PIBF) were analyzed by quantitative real-time PCR. It was observed that at lower concentrations of IFNT, the IFI16, l-selectin, ISG15, and PIBF were upregulated, whereas at higher concentrations the same were down regulated. At all the experimental concentrations, IFI44, OAS1, MX genes were significantly upregulated and CD31, CD11b were significantly downregulated. At lower concentrations of IFNT, the neutrophil activity with respect to chemoattraction is stimulated, whereas at higher concentrations the same is reduced. Hence, it can be concluded that IFNT exerts concentration-dependent actions on neutrophil gene-expression dynamics indicating a fine modulation of its activity depending upon the temporal variation in its destined functions ultimately leading to successful implantation.

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“…Expression CXCL10 mRNA is upregulated in bovine endometrium and PBLs on days 15 and 18 of pregnancy, and can be induced by IFNT in cultured endometrial tissue or PBLs [38,39]. Early pregnancy induces upregulation of CXCL10 gene in the endometria and PBMCs in sheep [40,41]. CXCL10 mRNA in endometrium and protein in the uterine flushing are increased from day 14 to 20 of pregnancy, which are involved in regulating endometrial functions through recruitment of immune cells in sheep [42].…”

Section: Discussionmentioning

confidence: 99%

Early Pregnancy Induces Expression of STAT1, OAS1 and CXCL10 in Ovine Spleen

Wang

Han

Zhang

et al. 2019

Animals

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Simple SummaryInterferon-tau is a maternal recognition factor in ruminants, and spleen plays an essential role in regulating innate and adaptive immune responses. We found that interferon-tau derived from conceptus induces expression of STAT1, OAS1, and CXCL10 in ovine maternal spleen, which may be helpful for maternal immune regulation.AbstractInterferon-tau is a maternal recognition factor in ruminant species, and spleen plays an essential role in regulating innate and adaptive immune responses. However, it is not fully understood that early pregnancy induces expression of interferon stimulated genes (ISGs) in the spleen during early pregnancy in ewes. In this study, spleens were collected from ewes at day 16 of the estrous cycle, and on days 13, 16, and 25 of gestation (n = 6 for each group), and RT-qPCR, western blot and immunohistochemistry analysis were used to detect the expression of signal transducer and activator of transcription 1 (STAT1), 2′,5′-oligoadenylate synthetase 1 (OAS1), myxovirusresistance protein 1 (Mx1) and C-X-C motif chemokine 10 (CXCL10). The results revealed that STAT1, OAS1 and CXCL10 mRNA and proteins were upregulated in the spleens during early pregnancy, and STAT1 protein was located in connective tissue cells in the capsule and trabeculae, and blood cells and lymphocytes in the red pulp. However, early pregnancy had no significant effects on expression of MX1 mRNA and protein. In conclusion, early pregnancy induces expression of STAT1, OAS1 and CXCL10 in maternal spleen, suggesting that maternal spleen is involved in immune regulation of pregnancy in sheep.

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Interferon stimulated genes as peripheral diagnostic markers of early pregnancy in sheep: a critical assessment

Cited by 20 publications

References 33 publications

Koyunların Erken Gebelik Döneminde Kemik iliğinde ISG15 Ekspresyonu

Koyunların Erken Gebelik Döneminde Kemik iliğinde ISG15 Ekspresyonu

Exploring the concentration‐dependent actions of interferon‐τ on bovine neutrophils to understand the process of implantation

Early Pregnancy Induces Expression of STAT1, OAS1 and CXCL10 in Ovine Spleen

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