Intergeneric pollen – megagametophyte relationships of conifers in vitro

Pollen characteristics and in vitro pollen germination of Cedrus libani A. Rich

“…Since the highest germination rate was achieved in MS medium, the experiments were continued in this medium (Table 1). Dumont-Beboux et al (1998) sterilized male flowers in 70% alcohol and subsequently with 1% sodium hypochloride for 30 s to eliminate the contamination. Although they achieved successful results, the most successful result in our experiments in terms of pollen surface sterilization was obtained by using higher amount of, that is, 5%, sodium hypochloride.…”

“…There are studies on the storage and in vitro germination of gymnosperm pollens (Copes et al, 1991;Lanteri et al, 1993;Dumont-Beboux et al, 1998;Fernando et al, 1998;Siregar and Sweet, 2000;Krouchi et al, 2004). The purpose of the study is to determine in vitro germination conditions of C. libani A.…”

Structure and function of the tentpole in the reproductive process of Ginkgo biloba L.

“…13,14 As another example, the sperm of Ceratopteris richardii extend to their full length once they are released from the antheridium, and their width decreases pollen tubes of Pinus monticola and Picea sitchensis may branch after arriving at the megagametophytes as a strategy to search for archegonia. 16 In G. biloba, just before fertilization, the proximal unbranched portion of the male gametophyte that is anchored to the pollen chamber swells and takes on a saccate appearance, characterizing maturation of the spermatozoid. 5 After release, the spermatozoid must swim from the pollen chamber and the archegonial chamber to the archegonium for successful fertilization.…”

Growth and development of conifer pollen tubes

“…Pollen tubes of Larix occidentalis, Picea sitchensis, and Pinus monticola were co-cultured with isolated female gametophytes from Pseudotsugamenziesii, Larix x eurolepis, and Pinus monticola (Dumont-BeBoux et al 1998). Using co-cultured pollen tubes and isolated female gametophytes, intra-and inter-specific crosses between Pinus aristata, P. monticola, and P. strobus were done .…”