Interleukin 1 Alpha and Beta in Psoriatic Skin: Enzymoimmunoassay, Immunoblot Studies and Effect of Systemic Retinoids

Gruaz, Dominique; Didierjean, Liliane; Grassi, Jacques; Frobert, Yveline; Dayer, Jean‐Michel; Saurat, Jean‐Hilaire

doi:10.1159/000248361

Cited by 41 publications

(33 citation statements)

References 9 publications

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“…[32][33][34][35][36][37] Interestingly, IL-1a, IL-1b and IL-1Ra produced by HKs and PKs were differentially regulated with respect to the above proinflammatory cytokines. IL-1a and IL-1Ra measured in the supernatants of PKs were reduced comparatively to HKs (PK/HK ¼ 0.5 and 0.2, respectively), while IL-1b was increased by a factor of 7.7.…”

mentioning

confidence: 93%

Pathological crosstalk in vitro between T lymphocytes and lesional keratinocytes in psoriasis: necessity of direct cell-to-cell contact

Martin¹,

Guérard²,

Fortin³

et al. 2012

Laboratory Investigation

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Psoriasis, a chronic autoimmune-related skin disease, involves both immune and non-immune cells like T cells and keratinocytes. This study investigates the regulatory role of T cells-keratinocyte interactions during psoriasis on immune factors production. Cytokines and chemokines were evaluated by multiplex and ELISA assays in an in vitro model of co-culture of keratinocytes with T lymphocytes. Keratinocytes were from psoriatic skin lesions or healthy skin. T lymphocytes were from healthy volunteers. Psoriatic keratinocytes (PKs) alone generated concentrations of tumor necrosis factor (TNF)-a, interleukin (IL)-6, granulocyte-macrophage colony-stimulating factor (GM-CSF), IL-1b, IL-8, monocyte chemotactic protein (MCP)-1, interferon-g-induced protein 10 kDa (IP-10) and vascular endothelial growth factor (VEGF) higher than those produced by healthy keratinocytes (HKs). In contrast, IL-1a and IL-Ra production was reduced in PKs. Normal T cells, which had no effect on HKs, increased the production of TNF-a, IL-6, GM-CSF, IL-8, MCP-1 and IP-10 by PKs, but did not influence PK production of IL-1b, IL-1a, IL-Ra and VEGF. The most striking effects were obtained with PK-and IL-2-stimulated T lymphocytes: most of the above cytokines and chemokines were greatly upregulated, except IL-1b and VEGF that were decreased or unchanged, respectively. In addition, fractalkine was overproduced in this latter condition only. Our results indicate (1) a functional interaction between keratinocytes and T lymphocytes that requires a direct cellular contact, and (2) a reciprocal influence that depends on cytokine and chemokine types. In conclusion, lesional keratinocytes from psoriasis vulgaris alter functions of normal T lymphocytes that conversely modulate these keratinocytes.

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mentioning

confidence: 93%

Pathological crosstalk in vitro between T lymphocytes and lesional keratinocytes in psoriasis: necessity of direct cell-to-cell contact

Martin¹,

Guérard²,

Fortin³

et al. 2012

Laboratory Investigation

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“…The role of IL-1, which is constitutively produced by normal human epidermal keratinocytes (Gahring et al 1985 ;Hauser et al 1986 ;Bell et al 1987), in psoriasis has been a great concern of many investigators (Camp et al 1986;Takematsu et al 1986 ;Gruaz et al 1989 ;Romero et al 1989). It has also not been settled whether the decreased IL-1 activity is due to an actual decrease or the presence of inhibitor of IL-1, and whether the biologic activity in the stmatum corneum is due to IL-la, f3, or both.…”

Section: Discussionmentioning

confidence: 99%

Decreased levels of IL-1.ALPHA. and .BETA. in psoriatic lesional skin.

Takematsu

Ohmoto²,

Tagami

1990

Tohoku J. Exp. Med.

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mediates a wide range of biological activities, is thought to play an important role in many inflammatory and immunologic diseases. Normal human epidermal keratinocytes constitutively produce IL-1. Based on our previous data indicating decreased IL-1 activity in psoriatic scale extracts, in the present study, we measured immunoreactive IL-1 a and ,3 levels in the suction blister fluids as well as in the psoriatic scale extracts using enzyme immunoassay for IL-la and /3. The results showed that although similarly low levels of IL-la were detectable in the suction blister fluid from normal and psoriatic Lesional skin, and that no IL-1f was found in most of the blister fluids, indicating that IL-i a is major IL-1 species produced by human skin. As compared to those in the blister fluids, IL-la levels in the horny tissue extracts were found to be much higher, and they were significantly higher in the orthokeratotic stratum corneum extracts than in the psoriatic scale extracts. However, gel filtration of the orthokeratotic horny tissue extracts demonstrated that constituents for immunoreactive IL-la and Q were quite variable depending upon the source of the horny tissues. The present study has confirmed that IL-1 levels in the psoriatic scale extracts are decreased when compared with those in the orthokeratotic horny tissue possibly due to an increased epidermal proliferation activity associated with its high turnover rate. The role of IL-1 psoriatic lesions remains unknown. psoriasis ; suction blister ; horny tissue ; interleukin 1; ETA Interleukin 1 (IL-1), which mediates a wide range of biological reactions, is thought to play an important role in many inflammatory and immunologic diseases ). On the basis of isoelectric points, two major forms of IL-1 have been identified : IL-la (pI 5.0) and /3 (7.0) (Auron et al. 1984 ;March et al. 1985 ;Gubler et al. 1986). Biological analyses of purified native and recombinant human IL-1a and /3 have shown that these proteins possess very similar, if not identical, bioactivities in a broad variety of bioassays (Wood et al.

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“…ILla levels were low but not decreased in suction blister fluids from PP skin [9]. In contrast to IL-la, cytosolic extracts of PP skin contained increased levels of a processed, but non-functional form of IL-16 [6,71. IL-1fi levels were negligible in extracts of the stratum corneum and in blister fluids [8, 91 and its expression seemed confined to the plasma membrane and the intracellular compartment of epidermal cells (EC) [lo, 111.…”

Section: Introductionmentioning

confidence: 87%

Enhanced production of biologically active interleukin‐1α and interleukin‐1β by psoriatic epidermal cells ex vivo: evidence of increased cytosolic interleukin‐1β levels and facilitated interleukin‐1 release

et al. 1995

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The expression of interleukin (1L)-1 is altered in psoriatic lesions. However, little is known about the actual production of IL-la and IL-lP by psoriatic epidermal cells (EC). We monitored IL-1 in the extracellular, the membrane and the intracellular compartment of freshly isolated E C from untreated lesional psoriatic (PP) and normal healthy (NN) skin during non-stimulated short-term cultures, representing a psoriasis model ex vivo. Cytokines were measured using bioassays combined with neutralizing antibodies and enzyme-linked immunosorbent assay in parallel. PP EC released significantly increased amounts of biologically active IL-la and IL-lP in a ratio of 3 : 1, whereas NN EC only released IL-la. Also, the release of IL-6, but not of TNF-a, by PP EC was significantly increased. Membrane-associated IL-1 activity, analyzed using glutaraldehydefixed EC, was low and not unique to PP EC. The cytosol of PP EC contained significantly increased levels of immunoreactive IL-1P. Furthermore, PP EC displayed loss of membrane integrity, as determined by trypan blue exclusion and release of cytosolic lactate dehydrogenase. This facilitated release of intracellular IL-1. Depletion of CD45+ cells showed that intraepidermal leukocytes did not contribute to the production of IL-1. Our observations show that resident PP E C express enhanced IL-1 production ex vivo, which is due to an increased cytosolic IL-1P content and facilitated IL-1 release. This study provides the first evidence that PP EC can produce bioactive IL-1p.

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Interleukin 1 Alpha and Beta in Psoriatic Skin: Enzymoimmunoassay, Immunoblot Studies and Effect of Systemic Retinoids

Cited by 41 publications

References 9 publications

Pathological crosstalk in vitro between T lymphocytes and lesional keratinocytes in psoriasis: necessity of direct cell-to-cell contact

Pathological crosstalk in vitro between T lymphocytes and lesional keratinocytes in psoriasis: necessity of direct cell-to-cell contact

Decreased levels of IL-1.ALPHA. and .BETA. in psoriatic lesional skin.

Enhanced production of biologically active interleukin‐1α and interleukin‐1β by psoriatic epidermal cells ex vivo: evidence of increased cytosolic interleukin‐1β levels and facilitated interleukin‐1 release

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