Interleukin 1 Polymorphisms Contribute to Intervertebral Disc Degeneration Risk: A Meta-Analysis

Wang, Zheng; Qu, Zhigang; Fu, Changfeng; Xu, Feng; Chen, Yong; Wang, Zhenyu; Liu, Yi

doi:10.1371/journal.pone.0156412

Cited by 18 publications

(19 citation statements)

References 39 publications

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“…It is often exposed to an inflammatory microenvironment. IL-1 β , a proinflammatory cytokine involved in inflammatory processes and the induction of apoptosis in response to cell injury, has been reported to have a connection with the degeneration of intervertebral discs [ 3 ]. Low back pain (LBP) is a frequent musculoskeletal disorder worldwide that affects approximately 70% of the adult population, sometime during their lives, and frequently results in musculoskeletal disability.…”

Section: Introductionmentioning

confidence: 99%

Icariin Prevents IL‐1β‐Induced Apoptosis in Human Nucleus Pulposus via the PI3K/AKT Pathway

Deng

Liang

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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Purpose To explore the effect and possible mechanism of icariin, a prenylated flavonol glycoside derived from the Chinese herb Epimedium sagittatum that was applied to IL-1β pretreated human nucleus pulposus (NP) cells. Methods Human NP cells were isolated from intervertebral discs of patients with scoliosis and lumbar spondylolisthesis. The cells were divided into five groups: A (blank control); B (20 ng/ml IL-1β); C (20 ng/ml IL-1β + 20 μM icariin); D (20 μM icariin + 20 ng/ml IL-1β + 25 μM LY294002); E (20 ng/ml IL-1β + 25 μM LY294002). For each of the five groups, the CCK8, apoptosis rates, ROS rates, and JC-1 rates were determined and an electron micrograph was performed. Different expression levels of apoptosis proteins and proteins in the PI3K/AKT pathway were detected via western blot. Results We found that the damage effects on human nucleus pulposus cells from 20 ng/ml of IL-1β exposure were attenuated by icariin. When the PI3K/AKT pathway was blocked by LY294002, a specific inhibitor of this pathway, the protective effect of icariin was impaired. In summary, icariin might be a protective traditional Chinese medicine, which prevents inflammation-induced degeneration of intervertebral discs partly through the PI3K/AKT pathway.

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Section: Introductionmentioning

confidence: 99%

Icariin Prevents IL‐1β‐Induced Apoptosis in Human Nucleus Pulposus via the PI3K/AKT Pathway

Deng

Liang

et al. 2017

Evidence-Based Complementary and Alternative Medicine

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“…Furthermore, the results of qRT-PCR and IHC analysis demonstrated that increased IL-1α and IL1-β expression levels were found in the degenerated disc. Wang et al initially reported a metaanalysis on IL-1α rs1800587 and IL-1β rs1143634 polymorphisms and LDD, which suggested that IL-1α rs1800587 polymorphism was signi cantly associated with the risk of disc degeneration [29]. There were several weaknesses in the previous meta-analysis.…”

Section: Discussionmentioning

confidence: 99%

Gene locus polymorphisms and expression levels of interleukin-1 in lumbar disc disease: a meta-analysis and immunohistochemical study

Jiang

Xiao

Zhang

et al. 2020

Preprint

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Background: To investigate the association between interleukin (IL)-1α (rs1800587), IL-1β (rs1143634) and IL-1 receptor antagonist (RN) variable number tandem repeat (VNTR) polymorphisms, expression levels and lumbar disc disease (LDD).Methods: All relevant articles were searched from 4 databases including PubMed, Embase, Web of Science and China National Knowledge Infrastructure (CNKI). Odds ratios (OR) with 95 % conﬁdence intervals (CI) were calculated to evaluate the association between IL-1 gene locus polymorphisms (rs1800587 in IL-1α, rs1143634 in IL-1β, VNTR in IL-1RN) and LDD susceptibility. Statistical analysis was conducted by Review Manager (Revman) 5.31 software. Furthermore, qRT-PCR and immunohistochemistry (IHC) were performed to evaluate IL-1α, IL-1β and IL-1RN expressions in the normal and degenerated disc.Results: A total of 15 case-control studies (1455 cases and 2362 controls) were included in our meta-analysis. The pooled results suggested that IL-1α rs1800587 polymorphism was associated with an increased risk of LDD in overall population (T vs. C, OR=1.21, 95%CI=1.04-1.40, P=0.01). The subgroup analysis found a significant association between IL-1β rs1143634 polymorphism and LDD in Asian population (T vs. C, OR=0.61, 95%CI=0.39-0.96, P=0.03). Results of qRT-PCR and IHC demonstrated that expressions of IL-1α and IL-1-β were significantly increased in the degenerated disc. (all P<0.05 )Conclusion: IL-1α rs1800587 and IL-1β rs1143634 polymorphisms were significantly associated with LDD in overall population and in Asian population, respectively.

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“…Polymorphisms in these genes were associated with susceptibility to other human diseases. The IL-1 gene cluster is located on chromosome 2q13–2q14.1 and is comprised of nine genes: IL-1α , IL-1β , the IL-1 receptor antagonist (IL-1RN) , and six IL-1-like factor genes ( IL-1F5 through IL-1F10 ) [ 31 ]. IL-1β is in a 70–110 kb region of chromosome 2q13–21 and consists of 7 exons and 6 introns [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

A Study of IL-1β, MMP-3, TGF-β1, and GDF5 Polymorphisms and Their Association with Primary Frozen Shoulder in a Chinese Han Population

Chen

Meng

Qian

et al. 2017

BioMed Research International

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Primary frozen shoulder (PFS) is a common condition of uncertain etiology that is characterized by shoulder pain and restriction of active and passive glenohumeral motions. The pathophysiology involves chronic inflammation and fibrosis of the joint capsule. Single nucleotide polymorphisms (SNPs) at IL-1β, MMP3, TGF-β1, and GDF5 have been associated with risk of a variety of inflammatory diseases; however, no studies have examined these SNPs with susceptibility to PFS. We investigated allele and genotype frequencies of rs1143627 at IL-1β, rs650108 at MMP-3, rs1800469 at TGF-β1, and rs143383 at GDF5 in 42 patients with PFS and 50 healthy controls in a Chinese Han population. Serum samples from both cohorts were evaluated to determine the expression levels of IL-1β. We found that the IL-1β rs1143627 CC genotype was associated with a decreased risk of PFS compared to the TT genotype (P = 0.022) and that serum IL-1β was expressed at a significantly higher level in the PFS cohort compared to that found in the control group (P < 0.001). Our findings indicated no evidence of an association between rs650108, rs1800469, or rs143383 and PFS. IL-1β is associated with susceptibility to PFS and may have a role in its pathogenesis in a Chinese Han population.

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Interleukin 1 Polymorphisms Contribute to Intervertebral Disc Degeneration Risk: A Meta-Analysis

Cited by 18 publications

References 39 publications

Icariin Prevents IL‐1β‐Induced Apoptosis in Human Nucleus Pulposus via the PI3K/AKT Pathway

Icariin Prevents IL‐1β‐Induced Apoptosis in Human Nucleus Pulposus via the PI3K/AKT Pathway

Gene locus polymorphisms and expression levels of interleukin-1 in lumbar disc disease: a meta-analysis and immunohistochemical study

A Study of IL-1β, MMP-3, TGF-β1, and GDF5 Polymorphisms and Their Association with Primary Frozen Shoulder in a Chinese Han Population

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