Interleukin 10 gene promoter polymorphisms in women with early-onset pre-eclampsia

Abstract

SummaryPre-eclampsia is one of the most serious disorders of human pregnancy and T helper type 1 (Th1)/Th2 imbalance plays a major role in its aetiology. The Th2 cytokine, interleukin (IL)-10, plays a significant role in the maintenance of pregnancy. The present study is aimed at understanding the role of IL-10 promoter polymorphisms (−1082 G/A; −592 A/C and −819 C/T) and their haplotypes in early-onset pre-eclampsia. A total of 120 patients and an equal number of women with normal pregnancy, from Government Maternity Hospital, Petlaburz, Hyderabad, India, were considered for the present study. A standard amplification refractory mutation system-polymerase chain reaction (ARMS-PCR) was carried out for genotyping followed by agarose gel electrophoresis. Appropriate statistical methods were applied to test for the significance of the results. It was found that the IL-10 −819 C allele (P = 0·003) and −592 A (P = 0·005) allele frequencies increased significantly in patients compared to controls. No significant difference was found with regard to −1082 promoter polymorphism. Haplotype analysis of the IL-10 single nucleotide polymorphisms (SNPs) revealed a significant association with ACC haplotype with a twofold increased risk in patients compared to controls. The frequencies of two common IL-10 haplotypes (GCC and ATA) did not show any significant difference. Further, the diplotype analysis revealed five genotypes: −1082A with −819C (P = 0·0016); −1082G with −819C (P = 0·0018); −819C with −592C (P = 0·001); −1082A with −592C (P = 0·032); and −1082G with −592C (P = 0·005) associated with the disease. These findings support the concept of contribution of IL-10 gene polymorphisms in the pathogenesis of early-onset pre-eclampsia.

Physiological predictions and the role of IL-10 -819 promoter polymorphism in preeclampsia

“…The three most important ones are -819 (C/T), -1082 (A/G), and -592 (C/A) (Jiang et al, 2015). In the present study (our case control study), established that the genotype distribution of the IL-10 gene promoter -819 (C/T) to pregnant women with preeclampsia in of Erbil Maternity and Pediatric Governmental Hospital is reliable with the results of a previous study 8 ZANCO Journal of Pure and Applied Sciences 2021 (Perrey et al, 1999) Therefore, this gene might conceivably be a candidate susceptibility gene in pre-eclampsia (Sowmya et al, 2014b).…”

“…Likewise, some studies have investigated the correlation of promoter polymorphisms with the circulating levels and placental levels of IL-10. Moreover, have shown that the genotype of IL-10 promoter may not play a significant role in the circulating of IL-10 levels, but has an effect on the placental levels of IL-10, suggesting that IL-10 plays an important role in proper placentation (Makris et al, 2006, Sowmya et al, 2014b.…”

Genetic, immune and vasoactive factors in the vascular dysfunction associated with hypertension in pregnancy

“…Studies have also shown possible association between clinical and laboratory manifestations of PE and the TNF-α gene G308A (rs1800629) polymorphism [17]. Other studies have shown specific IL-10 gene promoter polymorphisms in women with early-onset PE [18]. …”

Investigations into the association between polymorphisms in the interleukin-10 gene and risk of early-onset preeclampsia

“…Similarly, Sowmya et al (2014a) conducted a study in an Indian population, they suggested that the IL-10 -819T/C gene polymorphism could contribute to the risk of preeclampsia. Sowmya et al (2014b) conducted another study in Indian population, and they reported that IL-10 -819T/C and -592A/C played an important role in the pathogenesis of early-onset preelampsia. Pissetti et al (2014) suggested that IL-10 -1082A/G polymorphism was correlated with the development of preeclampsia.…”