Interleukin-12 augments a Th1-type immune response manifested as lymphocyte proliferation and interferon gamma production in Leishmania infantum-infected dogs

Strauss-Ayali, Dalit; Baneth, Gad; Shor, Sharon; Okano, Fumiyoshi; Jaffe, Charles L.

doi:10.1016/j.ijpara.2004.10.015

Cited by 49 publications

(48 citation statements)

References 54 publications

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“…The increase in IFN-γ transcripts in SLA-stimulated PBMC, contrasts with the lack of IFN-γ induction in polysymptomatic dogs. Experimentally L. infantum infected beagle dogs that exhibited mild clinical signs (oligosymptomatic) showed strong PBMC proliferation, IFN-γ production and mRNA expression in response to stimulation with L. infantum antigen (SLA) [39]. While PBMC from naturally infected polysymptomatic dogs still proliferate in response to SLA, this is minimal and IFN-γ production in response to Leishmania antigen was completely abolished [39].…”

Section: Discussionmentioning

confidence: 99%

“…Experimentally L. infantum infected beagle dogs that exhibited mild clinical signs (oligosymptomatic) showed strong PBMC proliferation, IFN-γ production and mRNA expression in response to stimulation with L. infantum antigen (SLA) [39]. While PBMC from naturally infected polysymptomatic dogs still proliferate in response to SLA, this is minimal and IFN-γ production in response to Leishmania antigen was completely abolished [39]. In the canine model, the expression of IFN-γ from PBMCs correlates with disease resistance/asymptomatic status in non-vaccinated animals [17,40]; further, IFN-γ is observed to increase and correlate with protection in vaccinated dogs [17,22,41].…”

Section: Discussionmentioning

confidence: 99%

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Immunogenicity of the P-8 amastigote antigen in the experimental model of canine visceral leishmaniasis

Carrillo

Ahmed

Goldsmith-Pestana

et al. 2007

Vaccine

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The P-8 proteoglycolipid complex , an amastigote antigen of L. pifanoi, has been demonstrated to induce protection in mouse models, as well as to induce Tc1/Th1-like cellular responses in American cutaneous leishmaniasis patients. Because the immunization with P-8 PGLC in the murine model does not appear to be genetically restricted, we have studied the reactivity of the P-8 PGLC in L. infantum infected dogs. In this study, it is shown that PBMC from experimentally infected dogs (asymptomatic, oligosymptomatic) significantly proliferated in response to soluble leishmanial antigen (SLA) or the P-8 PGLC. Further, quantification of the gene expression induced by the stimulation with P-8 in asymptomatically infected dogs showed an up-regulation of IFN-γ and TNF-α, which were three to four-fold higher than that induced by soluble Leishmania antigen (SLA). While no measurable induction of IL-10 was observed, low levels of IL-4 mRNA were observed in response to both P-8 and SLA antigens. Thus, our studies establish that P-8 is recognized by infected canines and elicits a potentially curative/protective Th1-like immune response. The identification of Leishmania antigens that elicit appropriate immune responses across different host species (humans, canine) and disease manifestations (cutaneous or visceral) could be an advantage in generating a general vaccine for leishmaniasis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Immunogenicity of the P-8 amastigote antigen in the experimental model of canine visceral leishmaniasis

Carrillo

Ahmed

Goldsmith-Pestana

et al. 2007

Vaccine

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“…Expression of IL-4 mRNA was found in the bone marrow of some symptomatic dogs [31], and significantly higher IL-4 expression was recently detected locally in skin lesions from L. infantum-infected dogs [7]. Interferon-γ expression of antigen stimulated PBMC, in skin lesions and in the bone marrow of infected dogs was shown to be elevated [7,10,31,41].…”

Section: Zoonotic Visceral Leishmaniasis (Zvl) Is Caused By Parasitesmentioning

confidence: 96%

“…The first group of dogs consisted of six male beagles (6 months old, Wt (mean ± SE) 12.58 ± 0.36 kg) that were studied following an experimental infection [40,41]. In brief, the dogs were infected intravenously with 8.6 × 10 8 L. infantum amastigotes.…”

Section: Animalsmentioning

confidence: 99%

Splenic immune responses during canine visceral leishmaniasis

Strauss-Ayali

Baneth

Jaffe³

2007

Vet. Res.

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-Dogs are the main reservoir host for zoonotic visceral leishmaniasis caused by Leishmania infantum. In this study we investigated the immune response in spleens of L. infantum-infected dogs by measuring the mRNA expression levels for a wide panel of cytokines, transcription factors and chemokines. mRNA levels and parasite load were followed during 7 months of experimental infection and 14 months post-treatment, and were compared to naturally-infected (NI) dogs. Similarly, serum anti-Leishmania IgG and IgG subclass levels were measured during experimental infection. An increase in IFN-γ, T-bet, IP-10 and RANTES was found in the experimentally and NI dogs, implicating a substantial type-1 immune response during canine visceral leishmaniasis. IL-4, a type-2 associated cytokine, increased as early as one month after experimental infection, while IL-5 was high at later stages. Interestingly, the expression levels of the Treg-associated cytokines, IL-10 and TGF-β, did not change during the infection. Total anti-Leishmania IgG and IgG subclasses increased during the experimental infection. However, no association with specific cytokine patterns was observed. Parasite load in the spleens increased as early as one month postinfection and remained high until treatment. The load was higher in the polysymptomatic NI dogs than in the experimentally-infected dogs. This study indicates that both type-1 and type-2 immune responses occur in the spleen during canine L. infantum infection, and suggests that the early elevation of IL-4 might have a role in the persistence of parasites in the presence of high IFN-γ expression.canine visceral leishmaniasis / IFN-γ / IL-4 / T-bet / IP-10

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“…On the other hand, high amounts of IL-12 can lead to differentiation and expansion of the CD4 ? Th1 cell subset [11], and thus resistance to infection. Parasite antigens presented through MHCI stimulate expansion of CD8 ?…”

Section: Introductionmentioning

confidence: 99%

Leishmania (Viannia) shawi purified antigens confer protection against murine cutaneous leishmaniasis

et al. 2011

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Interleukin-12 augments a Th1-type immune response manifested as lymphocyte proliferation and interferon gamma production in Leishmania infantum-infected dogs

Cited by 49 publications

References 54 publications

Immunogenicity of the P-8 amastigote antigen in the experimental model of canine visceral leishmaniasis

Immunogenicity of the P-8 amastigote antigen in the experimental model of canine visceral leishmaniasis

Splenic immune responses during canine visceral leishmaniasis

Leishmania (Viannia) shawi purified antigens confer protection against murine cutaneous leishmaniasis

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