Interleukin 4 and Interferon-Gamma Secretion by Antigen and Mitogen-Stimulated Mononuclear Cells in the Hyper-IgE Syndrome: No TH-2 Cytokine Pattern

Abnormal expression of CD54 in mixed reactions of mononuclear cells from hyper-IgE syndrome patients

“…Using a model of mixed lymphocyte reactions we did not find abnormalities in IL-2 and IFN-γ secretion by PBMCs from HIES patients, in agreement with other recent studies showing no significant differences in the secretion of these two cytokines in HIES (Rodriguez et al 1998, Chehimi et al 2001. This data particularly with respect to IFN-γ, contrasts with results published by others showing that mononuclear cells from HIES patients produce lower quantities of IFN-γ than healthy controls (Del Prete et al 1989, Paganelli et al 1991, Garraud et al 1999).…”

“…PBMCs -Production of several cytokines including IL-2, IFN-γ, IL-4, and others in supernatants from mitogen-stimulated cells from HIES patients has been addressed extensively with heterogeneous results (Del Prete et al 1989, Vercelli et al 1990, Paganelli et al 1991, Rousset et al 1991, Rodriguez et al 1998, Garraud et al 1999, Ohga et al 2003); yet no experimental evidence is available with respect to differences in cytokine production in HIES when T cells are activated using allogeneic stimulus. Therefore we evaluated the production of the cytokines IL-2 and IFN-γ in supernatants derived from PBMCs of HIES and controls using mytomicin-treated allogeneic PBMCs.…”

“…HIES patients exhibit nor- mal percentages of CD3+, CD4+, and CD8+ T cells and IgE-bearing B lymphocytes (Buckley 2001); in addition, most patients have decreased or absent cutaneous reactivity (delayed hypersensitivity reactions, DTH) to candidin, tetanus toxoid (TT) or diphteria toxoid. In vitro studies of lymphocyte function generally reveal normal proliferation responses to Concanavalin A, Pokeweed mitogen, and Phytohemagglutinin A but low or absent T lymphocyte proliferation in response to specific antigens such as Candida albicans, TT, and Dermatophagoides pteronnysinus (Leung & Geha 1988, Rodriguez et al 1998, Buckley 2001. Reduced percentages of CD45RO+ memory T-cells in peripheral blood from HIES patients has been reported only once (Buckley et al 1991), but the actual relevance of this finding or even the high IgE production in the patients' abnormal anamnesic humoral and cellular responses is presently unknown (Germain & Stefanova 1999).…”

“…Th2 CD4+ cells preferentially produce IL-4 promoting B-cell activation and isotype switch to IgE and IgG4 (Aversa et al 1993, Banchereau et al 1994. Studies involving the production and secretion of these cytokines and others such as IL-6, tumor necrosis factor (TNF), and IL-13 by antigen and mitogen-stimulated peripheral blood mononuclear cells (PBMCs) in HIES, have yielded conflicting results (Del Prete et al 1989, Vercelli et al 1990, Paganelli et al 1991, Rodriguez et al 1998, Garraud et al 1999. Recently, two independent groups have shown additional alterations in the IFN-γ/IL-12 pathways in HIES (Borges et al 2000, Chehimi et al 2001.…”

Evaluación de la función de los granulocitos en el síndrome de hiperinmunoglobulinemia E con infecciones recurrentes.

“…En los pacientes estudiados en este trabajo, la anormalidad descrita ha sido persistente, pues diferentes evaluaciones de la blastogénesis con toxoide tetánico, candidina y Dermatophagoides pteronyssinus han mostrado la misma alteración (25). Lo anterior refleja un patrón permanente de activación deficiente de los linfocitos T ante los retos antigénicos en este síndrome.…”