Interleukin-4 Single Nucleotide Polymorphism C-590t Polymorphisms in Relation to Asthma

Gaidan, Ayad M.; Abbas, Ahmed M.; Hassan, Mohammed; Hashim, Hashim

doi:10.22578/ijms.16.1.8

Cited by 3 publications

(3 citation statements)

References 16 publications

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“…The initial screening excluded studies that failed to meet the inclusion criteria. After ltering by availability and reading, consisting of reading the title, abstract, and keywords of each study, ve articles were selected [10][11][12][13][14] . The study selection process was visually presented in Fig.…”

Section: Characteristics and Quality Of Selected Studiesmentioning

confidence: 99%

“…However, they were still considered to be of moderate quality, scoring 5 or 6 points. HWE was detected in the asthmatic patients and controls in all studies [10][11][12][13][14] . All studies suggested that the distribution of genotypes in the controls was consistent with HWE except one study [10] , as shown in Table 3.…”

Section: Characteristics and Quality Of Selected Studiesmentioning

confidence: 99%

“…Five studies [10][11][12][13][14] with available data can be included to estimate the pool effect sizes under allele (C vs. T), homozygous (CC vs. TT), heterozygous (CT vs. TT) and recessive (CC vs. CT + TT) models. For the dominant (CC + CT vs. TT) model, there were ve studies [10][11][12][13][14] with available data that can be included to estimate the pool effect sizes. Although all these ve genetic models have a higher risk of asthma, the overall analyses were not statistically signi cant (Table 4).…”

Section: Meta-analysis and Sensitivity Analysis Findingmentioning

confidence: 99%

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Association between interleukin-4 C589T polymorphism and asthma in Iraq: Ameta-analysis

Hameed,

Abood

2024

Preprint

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Background Asthma is a common disease with a complex risk architecture involving both genetic and environmental factors. Several studies have identified an association between interleukin (IL)-4 C-589T and asthma risk; however, the results remain inconclusive. Aims to identify the effect of IL-4 C589T polymorphisms on asthma susceptibility in Iraq. Material and methods PubMed, Web of Science, Scopus, and Google Scholar databases were searched for only case-control studies published on Iraqi asthmatic. The methodological quality assessment of the included studies was performed based on the Newcastle-Ottawa Quality Scale (NOS). Based on the heterogeneity, we conducted a meta-analysis using random-effect models. Pooled odds ratios (ORs) with a 95% confidence interval (CI) were calculated using the allele (C vs. T), homozygous (CC vs. TT), heterozygous (CT vs. TT), dominant (CC þ CT vs. TT), and recessive (CC vs. CT þ TT) genetic models to assess the strength of the relationship between IL-4 C589T polymorphism and asthma risk. In addition, the stability of our analysis was evaluated by heterogeneity, sensitivity, and study design, and publication bias analysis. Result We included 5 case-control studies with a total of 359 cases and 245 controls. All genetic models showed a higher risk of asthma, the overall analyses were a highly statistically significant. There was moderate to high heterogeneity among the genetic models. However, sensitivity analysis revealed highly significant differences in the pooled odds ratios of the rs2243250 polymorphism between asthmatic patients and control groups. Conclusion The meta-analysis identified a significant association between the IL-4 C-589T polymorphism and asthma. However, the substantial result variability indicates the need for well-designed case-control studies with a large population to more accurately estimate this association.

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Section: Characteristics and Quality Of Selected Studiesmentioning

confidence: 99%

Section: Characteristics and Quality Of Selected Studiesmentioning

confidence: 99%

Section: Meta-analysis and Sensitivity Analysis Findingmentioning

confidence: 99%

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Association between interleukin-4 C589T polymorphism and asthma in Iraq: Ameta-analysis

Hameed,

Abood

2024

Preprint

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The Impact of Genetic variation at TNF-α -308 G/A on their serum production and severity of Asthma disease

Darweesh

Kadhem

2019

J. Phys.: Conf. Ser.

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Asthma is a chronic respiratory disease leading to morbidity and impaired quality of life worldwide. TNF-α cytokine responsible for the smooth muscle activation and late-phase airway hyper responsiveness. So this study aimed to investigate TNF-α polymorphisms situated at positions –308 G/A with their serum level and asthma severity. A case–control study included 40 asthmatic patients 16 females and 24 males and matched with age and gender 40 healthy subjects as a control group. Blood samples were collected from both groups, genomic DNA was extracted from peripheral blood for further molecular study. The study appeared that TNF-α-308 G/A significantly (P<0.05) high frequencies of homozygous AA, and A allele carrier in asthmatic patients, in contrast homozygous GG genotype and G allele appear at low frequency with patients. The data showed that genetic variants of the TNF-α affect the TNF-α production and associated with the severity of asthma, AA and AG genotype associated with high serum level (58.2 pg/ml and 49.5 pg/ml) respectively and GG genotype associated with low serum level (24.06pg/ml).The study confirmed the distinct role of TNF-α in cases of asthma compared to healthy controls with significantly higher level among patients, mean serum level for patients and control were (50.3 and 5.88 ) pg/ml respectively. Also, mean serum levels of TNF-α were significantly higher in asthmatic patients that suffering from allergic rhinitis(65.39 pg/ml) compared to non-rhinitis patients (34.97pg/ml). In conclusion, TNF-α-308 GG, genotype contribute to the predisposition of asthma and there was significant association among genotype of this gene and level of cytokine as well as development of the disease. Also observed an association between this gene and serum level of cytokine. AA, AG genotype and allergic rhinitis consider a risk factor of asthma and progress the disease.

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The association of FKBP5 polymorphism with asthma susceptibility in asthmatic patients

Alsaffar

Rasheed

Yenzeel

et al. 2021

Journal of Basic and Clinical Physiology and Pharmacology

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Objectives Inhaled corticosteroids are the most effective controllers of asthma, although asthmatics vary in their response. FKBP51 is a major component of the glucocorticoid receptor which regulates its responses to corticosteroids. Therefore, the present study aims to identify the role of FKBP5 gene polymorphism in asthma susceptibility and corticosteroid resistance. Methods DNA was extracted from the blood of 68 asthmatic and 40 control subjects. FKBP5 gene fragments were amplified by PCR and sequenced by the Sanger method. The sequencing results were aligned by mapping on the reference sequences of National center of Biotechnology Information (NCBI) and single nucleotide polymorphisms (SNPs) which were checked. Finally, the genotype, allele frequency and odds ratio (OR) were calculated. Results The FKBP5 fragment sequencing revealed the presence of rs1360780 and one novel SNP found in 17 samples taken from asthmatic patients as compared to db SNP data in the NCBI database. The FKBP5 variant (rs1360780) indicated that the allele frequency of risk allele T was 41.18% in patients and 20% in control group members p<0.001 and OR=2.8 when compared to a wild C allele frequency of 58.82% in patients and 64% in the control group members. The novel SNP FKBP5 was compared to the SNP database in the NCBI database in which wild T allele was substituted with G. The novel SNP was submitted to the ClinVar Submission Portal at NCBI with accession number: rs1581842283 and confirmed an asthma susceptibility risk factor with allele G frequency of 11.76% in asthmatics and 2.5% in the control group members (OR=5.2, p<0.05), as compared to a wild T allele frequency of 88.24% in asthmatics and 97.5% in the control group members. Conclusions The risk allele T of rs1360780 and the novel SNP rs1581842283 risk allele G predict asthma susceptibility but show no association with corticosteroid resistant.

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Interleukin-4 Single Nucleotide Polymorphism C-590t Polymorphisms in Relation to Asthma

Cited by 3 publications

References 16 publications

Association between interleukin-4 C589T polymorphism and asthma in Iraq: Ameta-analysis

Association between interleukin-4 C589T polymorphism and asthma in Iraq: Ameta-analysis

The Impact of Genetic variation at TNF-α -308 G/A on their serum production and severity of Asthma disease

The association of FKBP5 polymorphism with asthma susceptibility in asthmatic patients

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