Interleukin-6 gene polymorphisms and serum erythropoietin and hemoglobin in hemodialysis Iraqi patients

Al-Radeef, Mohanad Y.; Allawi, Ali A.; Fawzi, Hayder Adnan

doi:10.4103/1319-2442.243952

Cited by 13 publications

(13 citation statements)

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“…‎ A linear relationship is usually observed between the mass of red blood cells and ‎Hb saturation with oxygen in healthy peoples, while an inverse linear ‎relationship between serum EPO and Hb presents in chronically anemic ‎patients 1. Panjeta et al investigated 562 individual subjects for the correlation ‎between EPO, Hb and/or hematocrit based on the degree of renal insufficiency.…”

Section: Discussionmentioning

confidence: 99%

“…Chronic kidney disease (CKD) is common and continues to rise universally. ‎It is a risk factor for end-stage renal disease (ESRD) and is also a strong risk ‎factor for cardiovascular disease (CVD) and mortality 1. ESRD is a complex ‎disorder with a variety of phenotypes starting from a diversity of underlying ‎disorders of the kidney in conjunction with genetic and environmental factors in ‎addition to other preexisting or secondary clinical entities 2…”

Section: Introductionmentioning

confidence: 99%

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ACE gene polymorphism and its association with serum erythropoietin and hemoglobin ‎in Iraqi hemodialysis patients

Al-Radeef¹,

Fawzi²,

Allawi³

2019

TACG

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Purpose To evaluate the frequencies of angiotensin-converting enzyme gene ‎polymorphism in ‎Iraqi hemodialysis patients and to examine the association ‎between this polymorphism and ‎serum erythropoietin and hemoglobin levels.‎ Methods In this study, 70 chronic renal failure Iraqi patients on ‎maintenance ‎hemodialysis (patient group) and 20 healthy subjects (control ‎group) were genotyped for ‎ angiotensin-converting enzyme gene polymorphism. The distribution of genotype and allele ‎frequencies of this polymorphism in ‎these subjects were also evaluated.‎ Results The distribution of angiotensin-converting enzyme genotypes between ‎groups was ‎similar, and the ID genotype was the most frequent, followed by DD and II ‎genotypes (‎50%‎, ‎37%‎, and ‎13%). The control group ‎had a nonsignificant difference in serum ‎erythropoietin levels among different angiotensin-converting enzyme genotypes, ‎while patients with ID and DD genotypes displayed significant ‎elevation in ‎serum erythropoietin with time. No significant differences in hemoglobin ‎levels ‎were observed in patient and control groups. A significant positive correlation ‎was ‎observed between serum erythropoietin and hemoglobin in the control group ‎with different ‎angiotensin-converting enzyme genotypes, while a nonsignificant ‎negative correlation was ‎observed in the patient group throughout the study. ‎ Conclusions Chronic kidney disease did not significantly alter angiotensin-converting enzyme ‎genotypes, and angiotensin-converting enzyme gene ‎polymorphism had a significant effect on ‎serum erythropoietin levels and a non‎significant effect on hemoglobin levels. ‎

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

ACE gene polymorphism and its association with serum erythropoietin and hemoglobin ‎in Iraqi hemodialysis patients

Al-Radeef¹,

Fawzi²,

Allawi³

2019

TACG

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“…Genomic DNA was extracted from whole blood samples (baseline samples) using genomic DNA extraction kit (Reliaprep™ Blood gDNA Miniprep system, Promega, USA) and the quality of DNA was analyzed by agarose gel electrophoresis (AgaroPower™, Bioneer, Republic of Korea). Genotyping o'f IL-6 gene (˗174 G/C) was made with the aid of conventional polymerase' chain' reaction-restriction' fragment' length' polymorphism' (PCR-RFLP) (MyGenie™ 96 Thermal Block, Bioneer, Republic of Korea) according to the method of Al-Radeef et al (14). The following primers were used for PCR amplification: Forward: 5' TGA CTT CAG CTT TAC TCT TGT 3' Reverse: 5' CTG ATT GGA AAC CTT ATT AAG 3'…”

Section: Genotyping Of −174g/c Polymorphism Of Il-6 Genementioning

confidence: 99%

Association between Allelic Variations of -174G/C Polymorphism of Interleukin-6 Gene and Chronic Kidney Disease-Mineral and Bone Disorder in Iraqi Patients

Radeef

2020

Baghdad Sci.J

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This study designed to examine association between-174G/C polymorphism of interleukin-6 gene and phosphate, calcium, vitamin D3, and parathyroid hormone levels in Iraqi patient with chronic kidney disease on maintenance hemodialysis. Seventy chronic renal failure patients (patients group) and 20 healthy subjects (control group) were genotyped for interleukin-6 polymorphism and genotyping was performed by conventional polymerase chain reaction-restriction fragment length polymorphism. No significant differences in phosphate levels were observed in patients and control with different interleukin-6 genotypes. Control had non-significant differences in calcium levels, while patients with GG and CG genotypes displayed significant elevation with time. Conversely, control and patients with GG and CC genotypes had significant elevation in vitamin D3 levels with time. Regarding parathyroid hormone, control had non-significant differences, while patients with GG and CC genotypes displayed significant elevation with time. Patients with GG genotype displayed significant changes in calcium, vitamin D3 and parathyroid hormone levels with time.

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“…The mixture of the speci ic interaction for diagnosis gene consists of 5μl Taq PCR Pre Mix 10 picomols/μl (1μl) of Forward primer, 10 picomols/μl(1μl) of Reverse primer, 1.5μl of DNA, 16.5 μl of Distill water the inal volume is 25μl. The temperature has changed through the work of (Gradient PCR) for all samples to select the optimal condition, and also changed the concentration for DNA template between (1.5-2μl) where is considered these two factors from important factors in primer annealing with complement (Al-Radeef et al, 2018).…”

Section: Primer Design For Ubc Genementioning

confidence: 99%

Sequencing and structure analysis of UBC gene for breast and ‎lung cancer

Abdulhadi¹,

Mohammed²

2019

ijrps

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In the present study, sequencing approach has been adopted for exploring the ‎genetic alteration of sequences for the ubiquitin gene (UBC) in patients of breast and ‎lung cancer and comparing the results with a normal sequence that obtained from NCBI. ‎The aim of this study was to detect for genetic alterations of UBC gene in the breast and ‎lung cancer patients then compare with healthy control subjects, to investigate the ‎association between the mutations at the intron region of the UBC gene and cancer disease, ‎‎40 blood samples were examined from patients with breast and lung cancer aged ranged from (17-65) years, were collected at Al-Amal Hospital of cancer in Baghdad ‎province/Iraq, the period of collecting samples were from October/2018 to January/2019. ‎While twenty-two blood samples from healthy control subjects were collected at ages ‎ranged from(19-59). After DNA extraction, the PCR primer was designed to amplify the ‎region in the UBC gene (part of exon 1 and the whole intron). Here we report the polymorphism of the intron sequence of the UBC gene in Iraqi population as the results of sequencing the PCR amplified products showed three different transition mutation G→A, ‎C→T, T→C in patients with breast cancer were also appeared in healthy control subjects. While nine transition mutations appeared in lung cancer patients, at different locations ‎of the sequence were detected by BLAST tool. ‎

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Interleukin-6 gene polymorphisms and serum erythropoietin and hemoglobin in hemodialysis Iraqi patients

Cited by 13 publications

References 0 publications

<p><em>ACE</em> gene polymorphism and its association with serum erythropoietin and hemoglobin ‎in Iraqi hemodialysis patients</p>

<p><em>ACE</em> gene polymorphism and its association with serum erythropoietin and hemoglobin ‎in Iraqi hemodialysis patients</p>

Association between Allelic Variations of -174G/C Polymorphism of Interleukin-6 Gene and Chronic Kidney Disease-Mineral and Bone Disorder in Iraqi Patients

Sequencing and structure analysis of UBC gene for breast and ‎lung cancer

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