Interleukin-6 (IL-6) modulates migration and matrix metalloproteinase function in dermal fibroblasts from IL-6KO mice

Luckett, L.R.; Gallucci, Randle M.

doi:10.1111/j.1365-2133.2007.07867.x

Cited by 83 publications

(58 citation statements)

References 51 publications

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“…In the present study, VEGF-A, TGF-β1, and IL-6 were chosen to represent three different aspects of the wound healing process after different treatments, i.e., angiogenesis, fibrogenesis, and inflammation [40][41][42]. VEGF-A is an important growth factor involved in vasculogenesis and angiogenesis, which subsequently support the high metabolic activity for cell proliferation and collagen synthesis [40].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of genipin-crosslinked chitosan hydrogels as a potential carrier for silver sulfadiazine nanocrystals

Gao

Gan²,

Meng³

et al. 2016

Colloids and Surfaces B: Biointerfaces

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Section: Discussionmentioning

confidence: 99%

Evaluation of genipin-crosslinked chitosan hydrogels as a potential carrier for silver sulfadiazine nanocrystals

Gao

Gan²,

Meng³

et al. 2016

Colloids and Surfaces B: Biointerfaces

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“…Blockade of TNF‐α increased matrix synthesis and accelerated healing in murine models of age‐related impaired healing and excessive inflammation (Ashcroft et al ., 2012). IL6 can display pro‐ and anti‐inflammatory actions: IL6‐KO mice exhibit impaired granulation tissue formation and delayed cutaneous wound healing; IL6‐KO dermal fibroblasts had decreased production of matrix enzymes, suggesting an IL6 role in modulating fibroblast function (Luckett & Gallucci, 2007). One later study demonstrated that IL6Rα‐ERK signaling improved wound healing (McFarland‐Mancini et al ., 2010).…”

Section: Introductionmentioning

confidence: 99%

17β-estradiol ameliorates age-associated loss of fibroblast function by attenuating IFN-γ/STAT1-dependent miR-7 upregulation

et al. 2016

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SummaryAge‐related defects in fibroblast differentiation and functionality were previously shown to be associated with impaired hyaluronan (HA) synthase 2 (HAS2) and epidermal growth factor receptor (EGFR) function, as a result of upregulated microRNA‐7 (miR‐7) expression. In aging fibroblasts, inhibiting miR‐7 prevented the dysregulation of the HA‐mediated CD44/EGFR signaling pathway. Here, we investigated transcriptional upregulation of miR‐7 and implicated the age‐associated over‐activation of JAK/STAT1 as a primary candidate. STAT1 binding sites were identified on the putative miR‐7 promoter and stimulation of fibroblasts with the inflammatory cytokine, interferon‐γ (IFN‐γ), significantly increased miR‐7 transcriptional activity and resulted in upregulated miR‐7 and loss of EGFR. Additionally, we demonstrated a role for the anti‐inflammatory steroid, 17β‐estradiol (E2), in the attenuation of miR‐7 expression. E2 stimulation promoted estrogen receptor (ER) interactions with the miR‐7 putative promoter and suppressed miR‐7 expression. E2 also attenuated STAT1 expression and activity. Furthermore, treatments with E2 restored fibroblast functionality, including proliferation, migration and differentiation, key events in effective wound healing. In light of our findings, we propose that the regulation of miR‐7 by pro‐ and anti‐inflammatory mediators plays a wider role than previously thought. The modulation of fibroblast functions and ultimately wound healing by miR‐7 activators or inhibitors could provide realistic targets for the restoration of chronic wound healing capabilities in the elderly.

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“…Following 1 h of compression, there was a significant difference in IL-6 gene expression in the 20 kPa compressed sample compared to the non-compressed control group immediately following compression. IL-6 is known to enhance MMP-2 expression (Luckett and Gallucci 2007). Further studies are needed to Fig.…”

Section: Resultsmentioning

confidence: 95%

Effect of strain on human dermal fibroblasts in a three-dimensional collagen sponge

et al. 2013

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Our aim was to design a simple compression system and investigate the influence of mechanical stress on skin-like structures. Many mechanical compression studies have employed intricate culture systems, so the relationship between extracellular matrix material and the response of skin cells to mechanical stress remains unknown. Our approach uses only glass vials, 6-well plates and standard laboratory equipment. We examined the influence of mechanical stress on human skin fibroblasts embedded within a collagen sponge. The results show that mechanical compression increases MMP-1 and MMP-2 release by the cells into the the cell culture. Our results suggest that pressure on the skin may affect extracellular matrix degradation through some as yet unidentified pathways and that IL-6 mRNA expression may be involved in this effect. Using our approach, the effects of static mechanical stress on protein expression by cells in the culture medium and in sponges can be easily examined, and therefore this system will be useful for further analyses of skin responses to mechanical stress.

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Interleukin-6 (IL-6) modulates migration and matrix metalloproteinase function in dermal fibroblasts from IL-6KO mice

Abstract: These results indicate that IL-6 influences the function of fibroblasts in wounds, and one mechanism of this regulation may be through the modulation of MMP-2 and TIMP proteins.

Cited by 83 publications

References 51 publications

Evaluation of genipin-crosslinked chitosan hydrogels as a potential carrier for silver sulfadiazine nanocrystals

Evaluation of genipin-crosslinked chitosan hydrogels as a potential carrier for silver sulfadiazine nanocrystals

17β-estradiol ameliorates age-associated loss of fibroblast function by attenuating IFN-γ/STAT1-dependent miR-7 upregulation

Effect of strain on human dermal fibroblasts in a three-dimensional collagen sponge

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