Interleukin-8 and SDF1-alpha mRNA expression in colonic biopsies from patients with inflammatory bowel disease

Katsuta, Takeshi; Lim, Christina; Shimoda, Katsuhiro; Shibuta, Kenji; Mitra, Prasenjit; Banner, Barbara F.; Mori, Masaki; Barnard, Graham F.

doi:10.1111/j.1572-0241.2000.03289.x

Cited by 36 publications

(31 citation statements)

References 41 publications

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“…They have also been implicated in various pathological conditions such as cancer [36], malignant pleural effusion [37] and infection with the HIV [38]. Furthermore, their involvement in various inflammatory conditions, such as rheumatoid arthritis [39], thyroiditis [40], inflammatory bowel disease [41] and nephritis [42] has been reported. In these inflammatory diseases, SDF-1a/ CXCR4 axis contributes to their pathogenesis via its strong chemoattractant against T lymphocytes.…”

Section: Discussionmentioning

confidence: 99%

Clinical investigation: increased serum stromal derived factor 1 alpha levels in pulmonary tuberculosis THIS ARTICLE HAS BEEN RETRACTED

Mizuno

Matsuyama

Mitsuyama

et al. 2005

Clinical and Experimental Immunology

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SummaryPulmonary tuberculosis, a granulomatous disease, has few serological markers for its activity. Recently, an increased plasma level of stromal derived factor 1 alpha (SDF-1 a a a a ), which can induce strong chemotaxis of cells through its receptor CXCR4, was detected in patients with tuberculosis. In this study we investigated serum SDF-1 a a a a levels and CXCR4 expression on peripheral blood mononuclear cells (PBMCs). Fifty-five active tuberculosis patients, 30 resolved tuberculosis patients, 27 acute bronchitis patients and 8 healthy volunteers were examined. Histological expression of SDF-1 a a a a in the tuberculosis lesion and CXCR4 expression of PBMCs were also analysed. Serum SDF-1 a a a a levels in active tuberculosis patients were significantly higher than other groups. The sensitivity and specificity for the diagnosis of active tuberculosis was 88·5% and 85·3% (cutoff value = = = = 650 pg/ml), respectively. CXCR4 expression levels on PBMCs showed a significant negative correlation with serum SDF-1 a a a a levels. Inflammatory cells including multinuclear giant cells in the lesion expressed SDF-1 a a a a . Measurement of serum SDF-1 a a a a could be a useful screening marker for the identification of active pulmonary tuberuculosis. We propose that interaction of SDF-1 a a a a and CXCR4 might be involved in the pathogenesis of pulmonary tuberculosis.

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Section: Discussionmentioning

confidence: 99%

Clinical investigation: increased serum stromal derived factor 1 alpha levels in pulmonary tuberculosis THIS ARTICLE HAS BEEN RETRACTED

Mizuno

Matsuyama

Mitsuyama

et al. 2005

Clinical and Experimental Immunology

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“…‫,ء‬ p Ͻ 0.05 compared with the nontreated mice with DSS-induced colitis. at ASPET Journals on May 9, 2018 jpet.aspetjournals.org patients with UC was significantly higher than that in patients with CD (Katsuta et al, 2000). In addition, IL-4 induces surface CXCR4 expression on human T cells, suggesting that this receptor might be associated with T helper (Th)2 cells (Jourdan et al, 1998;Annunziato et al, 1999).…”

Section: Discussionmentioning

confidence: 99%

Blockade of CXCL12/CXCR4 Axis Ameliorates Murine Experimental Colitis

Mikami¹,

Nakase²,

Yamamoto³

et al. 2008

The Journal of Pharmacology and Experimental Therapeutics

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Recent studies indicate that the CXCL12/CXCR4 interaction is involved in several inflammatory conditions. However, it is unclear whether this interaction has a role in the pathophysiology of inflammatory bowel disease (IBD). We investigated the significance of this interaction in patients with IBD and in mice with dextran sulfate sodium (DSS)-induced colitis and the effect of a CXCR4 antagonist on experimental colitis. First, we measured CXCR4 expression on peripheral T cells in patients with IBD.

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“…However, overall, statistically significant differences in the extent or pattern of citrullination between normal and diseased tissue could not be observed (58). CXCL12 mRNA expression was investigated in homogenized colonic biopsies from patients with inflammatory bowel diseases and CXCL12 mRNA levels were higher in patients suffering from ulcerative colitis than from Crohn's disease (59). We studied protein expression and observed a regional expression pattern of CXCL12 protein in Crohn's disease showing marked up-regulation in areas of ulceration and erosion compared with normal colon.…”

Section: Discussionmentioning

confidence: 99%

Citrullination of CXCL12 Differentially Reduces CXCR4 and CXCR7 Binding with Loss of Inflammatory and Anti-HIV-1 Activity via CXCR4

Struyf

Noppen

Loos

et al. 2009

The Journal of Immunology

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Posttranslational proteolytic processing of chemokines is a natural mechanism to regulate inflammation. In this study, we describe modification of the CXC chemokine stromal cell-derived factor 1α/CXCL12 by peptidylarginine deiminase (PAD) that converts arginine residues into citrulline (Cit), thereby reducing the number of positive charges. The three NH2-terminal arginines of CXCL12, Arg8, Arg12, and Arg20, were citrullinated upon incubation with PAD. The physiologic relevance of citrullination was demonstrated by showing coexpression of CXCL12 and PAD in Crohn’s disease. Three CXCL12 isoforms were synthesized for biologic characterization: CXCL12-1Cit, CXCL12-3Cit, and CXCL12-5Cit, in which Arg8, Arg8/Arg12/Arg20, or all five arginines were citrullinated, respectively. Replacement of only Arg8 caused already impaired (30-fold reduction) CXCR4 binding and signaling (calcium mobilization, phosphorylation of ERK and protein kinase B) properties. Interaction with CXCR4 was completely abolished for CXCL12-3Cit and CXCL12-5Cit. However, the CXCR7-binding capacities of CXCL12-1Cit and CXCL12-3Cit were, respectively, intact and reduced, whereas CXCL12-5Cit failed to bind CXCR7. In chemotaxis assays with lymphocytes and monocytes, CXCL12-3Cit and CXCL12-5Cit were completely devoid of activity, whereas CXCL12-1Cit, albeit at higher concentrations than CXCL12, induced migration. The antiviral potency of CXCL12-1Cit was reduced compared with CXCL12 and CXCL12-3Cit and CXCL12-5Cit (maximal dose 200 nM) could not inhibit infection of lymphocytic MT-4 cells with the HIV-1 strains NL4.3 and HE. In conclusion, modification of CXCL12 by one Cit severely impaired the CXCR4-mediated biologic effects of this chemokine and maximally citrullinated CXCL12 was inactive. Therefore, PAD is a potent physiologic down-regulator of CXCL12 function.

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Interleukin-8 and SDF1-alpha mRNA expression in colonic biopsies from patients with inflammatory bowel disease

Abstract: IL-8 but not SDF1-alpha mRNA expression was associated with inflammation in UC. This suggests that IL-8 may play a more important role in inflammatory bowel disease than does SDF1-alpha.

Cited by 36 publications

References 41 publications

Clinical investigation: increased serum stromal derived factor 1 alpha levels in pulmonary tuberculosis THIS ARTICLE HAS BEEN RETRACTED

Clinical investigation: increased serum stromal derived factor 1 alpha levels in pulmonary tuberculosis THIS ARTICLE HAS BEEN RETRACTED

Blockade of CXCL12/CXCR4 Axis Ameliorates Murine Experimental Colitis

Citrullination of CXCL12 Differentially Reduces CXCR4 and CXCR7 Binding with Loss of Inflammatory and Anti-HIV-1 Activity via CXCR4

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