Interleukin-8 can mediate acute-phase protein production by isolated human hepatocytes

Wigmore, Stephen J.; Fearon, K. C. H.; Maingay, Jean P.; Lai, Paul Bo San; Ross, James A.

doi:10.1152/ajpendo.1997.273.4.e720

Cited by 93 publications

(84 citation statements)

References 22 publications

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“…Thus these cytokines could be implicated in a specific response to sole ulcers. In turn, IL-1β can increase expression of CXCL8 mRNA in leukocytes (Fujishima et al, 1993), and both IL-1β and CXCL8 are implicated in activating haptoglobin secretion (Godson et al, 1995;Wigmore et al, 1997). This is in agreement with the tendency for higher serum haptoglobin, CXCL8 mRNA, and haptoglobin mRNA expression in the lame cows in this study.…”

Section: Discussionsupporting

confidence: 89%

Differences in leukocyte profile, gene expression, and metabolite status of dairy cows with or without sole ulcers

O’Driscoll

McCabe

Earley

2015

Journal of Dairy Science

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Interpretive summary Sole ulcers affect dairy cow immune status by O'Driscoll et alDairy cows afflicted with sole ulcers are often clinically lame, and experience pain and impaired welfare. However little is known of the cows' stress and immune response to sole ulcers. This study demonstrated that cows that are affected with sole ulcers, and clinically lame, had a different leukocyte gene expression profile to healthy cows. They also had higher concentrations of the stress hormone, cortisol, the endogenous precursor steroid hormone -DHEA, and the various metabolites that are suggestive of systemic inflammation and stress. ABSTRACTSole ulcers are one of the most severe lameness causing pathologies for dairy cows and are associated with abnormal behaviour and impaired production performance. However, little is known about how or whether lameness caused by sole ulcers affects the cow systemically. This study compared haematology profile, leukocyte gene expression, and physiological responses (metabolite, cortisol, the endogenous steroid hormone -DHEA, and haptoglobin concentrations) of cows with sole ulcers and healthy cows. Twelve clinically lame cows (LAME) were identified as having at least one sole ulcer, and no other disorder and matched with a cow that had good locomotion and no disorders (SOUND), using days in milk, liveweight, body condition score and diet. Blood samples were taken from all 24 cows within 24h of sole ulcer diagnosis. Leukocyte counts were obtained using an automated cell counter, cortisol and DHEA concentration by ELISA, and plasma haptoglobin (Hb), urea, total protein, creatine kinase and glucose were analysed on an Olympus analyser. Expression of 16 genes associated with lameness or stress were estimated using qRT-PCR. Data were analysed using the Mixed procedure in SAS (v 9.3). LAME cows had a higher neutrophil %, a numerically lower lymphocyte %, and tended to have a higher neutrophil:lymphocyte ratio than SOUND. Serum cortisol and DHEA concentrations were higher in LAME than in SOUND cows. LAME cows also tended to have higher haptoglobin and glucose levels than SOUND, as well as higher protein yet lower urea levels. SOUND cows tended to have higher relative expression of the gene coding for colony stimulating factor 2 than LAME, but in all other cases where differences were detected in cytokine gene expression (IL-1α, IL-1β, CXCL8 and IL-10), relative gene expression in SOUND cows tended to be, or was, lower than in LAME.Relative expression of MMP-13, GR-α, Fas, haptoglobin and CD62L were, or tended to be, higher in LAME than SOUND cows. A high neutrophil:lymphocyte ratio in combination with higher cortisol levels in cows with ulcers is indicative of physiological stress. Moreover, increased DHEA and a higher cortisol:DHEA ratio, as well as a tendency for higher haptoglobin levels and increased 5 haptoglobin mRNA expression are indicative of systemic inflammation. Increased cytokine mRNA expression indicates activation of the immune system compared with healthy cows. Increased express...

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Section: Discussionsupporting

confidence: 89%

Differences in leukocyte profile, gene expression, and metabolite status of dairy cows with or without sole ulcers

O’Driscoll

McCabe

Earley

2015

Journal of Dairy Science

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“…20 Human hepatoma cell line HepG2 is able to produce CRP Ϸ4-fold compared with control in conditions similar to those used in our experiments. 21 Another hepatoma cell line Hep3B stimulated with conditioned medium (generated by stimulating peripheral blood mononuclear cell with LPS at a dose of 1 g/mL for 24 hours) showed an Ϸ50-fold increase in CRP mRNA compared with unstimulated cells.…”

Section: Discussionmentioning

confidence: 64%

Inflammatory Cytokines Stimulated C-Reactive Protein Production by Human Coronary Artery Smooth Muscle Cells

2003

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Background-Serum C-reactive protein (CRP) levels are good predictors of the development of cardiovascular events in apparently healthy men and women. CRP has been believed to be produced exclusively by hepatocytes during the acute-phase response. Several lines of evidence have suggested that atherosclerotic arteries can also produce CRP. However, the cell types that produce CRP locally in the atherosclerotic arterial wall have not been clearly identified. Methods and Results-Human coronary artery smooth muscle cells (HCASMCs) and human umbilical vein endothelial cells (HUVECs) were incubated with interleukin-1␤ (IL-1␤), IL-6, their combination, tumor necrosis factor-␣ (TNF-␣), or lipopolysaccharide (LPS) at different concentrations. The supernatants were concentrated and analyzed by a high-sensitivity enzyme-linked immunosorbent assay specific for human CRP. RNA was extracted from the HCASMCs for reverse transcriptase-polymerase chain reaction (RT-PCR) using specific primers for the CRP. Maximal CRP production was observed in HCASMCs after 48 hours of incubation with the combination of 25 ng/mL of IL-1␤ and 10 ng/mL of IL-6, whereas incubation with IL-1␤ or IL-6 alone only modestly induced CRP. Incubation with TNF-␣ (50 ng/mL) or LPS (1000 EU/mL) resulted in an increase in CRP production comparable to the IL-1␤ and IL-6 combination. [1][2][3][4] and could directly participate in the pathogenesis of atherosclerosis through activation of endothelial cells. 5-9 CRP, named for its capacity to bind to the C-polysaccharide of Streptococcus pneumoniae, was the first acute-phase protein to be described. 10 CRP, like other acute-phase proteins, is synthesized by the liver in response to microbial infection, tissue injury, and autoimmune disorders. It had been shown that interleukin-1␤ (IL-1␤) and IL-6 strongly induced the expression of CRP in human hepatocytes 11 and hepatoma cells. 12 Recently, human neuronal cells were found to produce CRP in Alzheimer's disease. 13 In addition, renal cortical tubular epithelial cells were shown to produce CRP after inflammatory stimuli. 14 Interestingly, CRP has also been found in human atherosclerotic plaques, 15 which could be the result of indirect deposit from circulating cells or direct production by cells in the arterial wall. We show that human coronary artery smooth muscle cells (HCASMCs), but not human umbilical vein endothelial cells (HUVECs), can synthesize CRP after stimulation by inflammatory cytokines. Methods Cell CultureHCASMCs, HUVECs, and endothelial cell supplements were purchased from Cascade Biologics; penicillin, streptomycin, medium 231, medium 199, and smooth muscle cell growth supplement were from Gibco BRL; and fetal bovine serum, human serum, heparin, and gelatin were obtained from Sigma. HCASMCs were plated onto 0.1% gelatin-coated culture dishes from Corning, Inc, and grown in 231 medium with growth supplement and 1% penicillin/streptomycin; HUVECs were plated onto 0.1% gelatin-coated culture dishes and grown in 199 medium with endothelial growth suppleme...

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“…IL-8 has not been isolated from rats, and CINC-1 is considered to be the rat counterpart of human IL-8 [25,26]. IL-8 was confirmed to mediate the production of acute-phase proteins in isolated human hepatocytes [28]. The serum levels of α2M after injection of the IL-6-or CINC-1-rich fractions increased; however, no significant differences were observed when compared with controls.…”

Section: Discussionmentioning

confidence: 99%

The Effects of Interleukin-6 and Cytokine-Induced Neutrophil Chemoattractant-1 on .ALPHA.2-Macroglobulin Production in Rats

et al. 2010

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Abstract:We investigated the effects of interleukin-6 (IL-6) and cytokine-induced neutrophil chemoattractant-1 (CINC-1) on α 2 -macroglobulin (α2M) production in rats. IL-6-rich and CINC-1-rich fractions were separated from serum obtained from rats 12 h after injection with turpentine oil using gel-chromatography. Sexual dimorphism was observed in the peak levels of α2M after injection of IL-6-, CINC-1-, or a mixture of IL-6-and-CINC-1-rich fractions. No significant differences in α2M levels were observed in males after injection with IL-6-or CINC-1-rich fractions and those injected with normal serum obtained from healthy rats (control). In contrast, serum levels of α2M, 6 to 120 h after injection of a mixture of IL-6-and CINC-1-rich fractions were significantly higher than in control rats. These results suggest that IL-6 and CINC-1 contribute to α2M production in rats only when IL-6 and CINC-1 act synergistically.

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Interleukin-8 can mediate acute-phase protein production by isolated human hepatocytes

Cited by 93 publications

References 22 publications

Differences in leukocyte profile, gene expression, and metabolite status of dairy cows with or without sole ulcers

Differences in leukocyte profile, gene expression, and metabolite status of dairy cows with or without sole ulcers

Inflammatory Cytokines Stimulated C-Reactive Protein Production by Human Coronary Artery Smooth Muscle Cells

The Effects of Interleukin-6 and Cytokine-Induced Neutrophil Chemoattractant-1 on .ALPHA.2-Macroglobulin Production in Rats

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