Interleukins 4 and 21 Protect Anti-IgM Induced Cell Death in Ramos B Cells: Implication for Autoimmune Diseases

Hui, Chin Wai; Wu, Wai Chung; Leung, Shui On

doi:10.3389/fimmu.2022.919854

Cited by 8 publications

(4 citation statements)

References 107 publications

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“…IL4 levels were elevated in all four groups of patients with autoimmune diseases (rheumatoid arthritis, Sjögren's syndrome, systemic lupus erythematosus and ulcerative colitis) (Supplemental Fig. S6), consistent with the role of this protein in B-cell hyperactivity, which mediates a broad spectrum of autoimmune diseases 22 . IL17A and IL17F are cytokines secreted by T helper 17 cells that play crucial roles in the was not certified by peer review) is the author/funder.…”

Section: Nulisaseq Comparison With Other Immunoassayssupporting

confidence: 71%

NULISA: a novel proteomic liquid biopsy platform with attomolar sensitivity and high multiplexing

Feng¹,

Beer²,

Hao³

et al. 2023

Preprint

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The blood proteome holds great promise for precision medicine but poses daunting challenges due to the low abundance of the majority of plasma proteins and the vast dynamic range across the proteome. We report the development and validation of a novel proteomic analysis technology - NUcleic acid Linked Immuno-Sandwich Assay (NULISA™) - that incorporates a dual capture and release mechanism to suppress the assay background to the minimum, thus drastically improving the signal-to-noise ratio. NULISA improves the sensitivity of the proximity ligation assay by over 10,000-fold to the attomolar level, which is enabled by antibody-conjugated DNA sequences that mediate the purification of immunocomplexes and contain target- and sample-specific barcodes for next-generation sequencing-based, highly multiplexed analysis. To demonstrate its performance and utility, we developed a 200-plex NULISA targeting 124 cytokines and chemokines and 80 other immune response-related proteins that demonstrated superior sensitivity for detecting low-abundance proteins and high concordance with other immunoassays. The ultra-high sensitivity enabled the detection of previously difficult-to-detect but biologically important, low-abundance biomarkers in patients with autoimmune diseases and COVID-19. Fully automated NULISA uniquely addresses longstanding challenges in the proteomic analysis of liquid biopsy samples and makes broad and in-depth proteomic analysis accessible to the general research community and future diagnostic applications.

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Section: Nulisaseq Comparison With Other Immunoassayssupporting

confidence: 71%

NULISA: a novel proteomic liquid biopsy platform with attomolar sensitivity and high multiplexing

Feng¹,

Beer²,

Hao³

et al. 2023

Preprint

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show abstract

“…Discontinuous fragmentation of nuclear DNA during apoptosis is revealed by discrete “Sub-G1” peaks on DNA content histograms [ 94 ]. Cells accumulated in the sub-G0/G1 phase are classified as apoptotic cells with DNA fragmentation and mitochondrial dysfunction [ 95 ].…”

Section: Resultsmentioning

confidence: 99%

ROS-Induced DNA-Damage and Autophagy in Oral Squamous Cell Carcinoma by Usnea barbata Oil Extract—An In Vitro Study

Popovici

Musuc

Matei

et al. 2022

IJMS

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Oxidative stress is associated with aging, cancers, and numerous metabolic and chronic disorders, and phenolic compounds are well known for their health-promoting role due to their free-radical scavenging activity. These phytochemicals could also exhibit pro-oxidant effects. Due to its bioactive phenolic secondary metabolites, Usnea barbata (L.) Weber ex. F.H. Wigg (U. barbata) displays anticancer and antioxidant activities and has been used as a phytomedicine for thousands of years. The present work aims to analyze the properties of U. barbata extract in canola oil (UBO). The UBO cytotoxicity on oral squamous cell carcinoma (OSCC) CLS-354 cell line and blood cell cultures was explored through complex flow cytometry analyses regarding apoptosis, reactive oxygen species (ROS) levels, the enzymatic activity of caspase 3/7, cell cycle, nuclear shrinkage (NS), autophagy (A), and synthesis of deoxyribonucleic acid (DNA). All these studies were concomitantly performed on canola oil (CNO) to evidence the interaction of lichen metabolites with the constituents of this green solvent used for extraction. The obtained data evidenced that UBO inhibited CLS-354 oral cancer cell proliferation through ROS generation (316.67 × 104), determining higher levels of nuclear shrinkage (40.12%), cell cycle arrest in G0/G1 (92.51%; G0 is the differentiation phase, while during G1 phase occurs preparation for cell division), DNA fragmentation (2.97%), and autophagy (62.98%) than in blood cells. At a substantially higher ROS level in blood cells (5250.00 × 104), the processes that lead to cell death—NS (30.05%), cell cycle arrest in G0/G1 (86.30%), DNA fragmentation (0.72%), and autophagy (39.37%)—are considerably lower than in CLS-354 oral cancer cells. Our work reveals the ROS-mediated anticancer potential of UBO through DNA damage and autophagy. Moreover, the present study suggests that UBO pharmacological potential could result from the synergism between lichen secondary metabolites and canola oil phytoconstituents.

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“…9). IL4, IL17 and IL33 all have well established roles in autoimmune diseases [21][22][23] . These results thus indicate that the higher sensitivity of NULISAseq can identify more clinically important but low-abundance biomarkers.…”

Section: Nulisaseq Comparison With Other Immunoassaysmentioning

confidence: 99%

NULISA: a proteomic liquid biopsy platform with attomolar sensitivity and high multiplexing

Feng,

Beer,

Hao

et al. 2023

Nat Commun

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The blood proteome holds great promise for precision medicine but poses substantial challenges due to the low abundance of most plasma proteins and the vast dynamic range of the plasma proteome. Here we address these challenges with NUcleic acid Linked Immuno-Sandwich Assay (NULISA™), which improves the sensitivity of traditional proximity ligation assays by ~10,000-fold to attomolar level, by suppressing assay background via a dual capture and release mechanism built into oligonucleotide-conjugated antibodies. Highly multiplexed quantification of both low- and high-abundance proteins spanning a wide dynamic range is achieved by attenuating signals from abundant targets with unconjugated antibodies and next-generation sequencing of barcoded reporter DNA. A 200-plex NULISA containing 124 cytokines and chemokines and other proteins demonstrates superior sensitivity to a proximity extension assay in detecting biologically important low-abundance biomarkers in patients with autoimmune diseases and COVID-19. Fully automated NULISA makes broad and in-depth proteomic analysis easily accessible for research and diagnostic applications.

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Interleukins 4 and 21 Protect Anti-IgM Induced Cell Death in Ramos B Cells: Implication for Autoimmune Diseases

Cited by 8 publications

References 107 publications

NULISA: a novel proteomic liquid biopsy platform with attomolar sensitivity and high multiplexing

NULISA: a novel proteomic liquid biopsy platform with attomolar sensitivity and high multiplexing

ROS-Induced DNA-Damage and Autophagy in Oral Squamous Cell Carcinoma by Usnea barbata Oil Extract—An In Vitro Study

NULISA: a proteomic liquid biopsy platform with attomolar sensitivity and high multiplexing

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