Intermittent Feeding as A Factor Enhancing Hemopoietic Stem Cell Proliferation and Spleen Colony Formation in Irradiated Mice

Abstract

The influence of metabolic stimulation induced by a 3 weeks' adaptation of the animals to intermittent food intake on hemopoietic stem cells was investigated in mice. The methods used included transplantation of bone marrow to lethally irradiated recipients, assay of CFUs number, seeding efficiency, and incorporation of '*'iododeoxyuridine into the DNA of spleen cells. A stimulatory effect of the metabolically influenced hemopoietic environment on the proliferative activity in stem cell compartments and on the recovery of hemopoietic organs was demonstrated. These stimulatory effects were most marked when the bone marrow of metabolically influenced donors was transplanted to similarly influenced recipients. .

NRF2 and the Phase II Response in Acute Stress Resistance Induced by Dietary Restriction

“…Three major conclusions are evident: 1) DR and DR mimetics are powerful tools to prevent damage from a variety of acute stressors including ischemia/ischemic reperfusion injury [84–87], toxic levels of chemicals and drugs [88–93], trauma [94], infection [95], inflammation [96] and radiological damage [9,2] 2) different DR regimens (type, duration, dose) and mimetics can provide protection against the same stressors across a variety of different species; and 3) maintenance of cellular antioxidant, detoxification and redox cycling programs is a common theme in protection against acute stress. These same pathways are also intricately involved in the Phase II response to toxicological and electrophilic stress, as described below.…”

Sulfur Amino Acid Supplementation Abrogates Protective Effects of Caloric Restriction for Enhancing Bone Marrow Regrowth Following Ionizing Radiation

“…Long-term periodic fasting, a specific form of CR, between 2 to 12 months prevents age-dependent declines in immune cells, platelets, hemoglobin, and red blood cells [ 17 ], and protects bone HSCs from genotoxic chemotherapy by promoting proper HSC self-renewal [ 18 ]. In addition to delaying aging-related pathologies, long-term periodic fasting for 3 weeks in mice prior to ionizing radiation exposure results in improved femoral bone marrow repopulation and HSPC recovery within one to two weeks post irradiation [ 19 , 20 ], and decreases radiation induced death [ 21 ]. Additionally, CR lasting between 4 weeks and the entire adult lifetime prior to, concurrent with, and/or after ionizing radiation exposure reduces the incidence of secondary tumors and improves survival in rodents [ 22 , 23 ].…”