Intermittent Hypoxic Exposure with High Dose of Arginine Impact on Circulating Mediators of Tissue Regeneration

Zembroń-Łacny, Agnieszka; Gramacki, Artur; Wawrzyniak-Gramacka, Edyta; Tylutka, Anna; Hertmanowska, Natalia; Kasperska, Anna; Czuba, Miłośz

doi:10.3390/nu12071933

Cited by 4 publications

(5 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Using correlation analysis, Sonnweber et al (2014) found that PDGF is associated with hypoxia mediated hepcidin repression in humans. Interestingly, Zembroń-Łacny et al (2020) recently showed that PDGF remained significantly higher 1 day after the passive 12-day intermittent hypoxic exposure, suggesting that PDGF could potentially have contributed to maintaining the downregulation of hepcidin observed in our study. However, we did not measure PDFG’s response to the LH-TL protocol–this is worth considering for future research.…”

Section: Discussionsupporting

confidence: 59%

Hepcidin and erythroferrone response to 3 weeks of exposure to normobaric hypoxia at rest in trained cyclists

Płoszczyca,

Czuba,

Chalimoniuk

et al. 2023

Front. Physiol.

Self Cite

View full text Add to dashboard Cite

Purpose: The effectiveness of altitude training on haematological adaptations is largely dependent on iron metabolism. Hepcidin and erythroferrone (ERFE) are key iron-regulating hormones, yet their response to altitude training is poorly understood. The aim of this study was to analyze changes in hepcidin and ERFE under the influence of 3 weeks of the Live High-Train Low (LH-TL) method.Methods: Twenty male trained cyclists completed a 3-week training program under normoxic conditions (NORM) or with passive exposure to normobaric hypoxia (LH-TL; FiO2 = 16.5%, ∼2000 m; 11–12 h/day). Hepcidin, ERFE, hypoxia inducible factor-2 (HIF-2), ferroportin (Fpn), erythropoietin (EPO), serum iron (Fe) and hematological variables were assessed at baseline (S1), then immediately after (S2) and 3 days after (S3) intervention.Results: In the LH-TL group, hepcidin decreased by 13.0% (p < 0.001) in S2 and remained at a reduced level in S3. ERFE decreased by 28.7% (p < 0.05) in S2 and returned to baseline in S3. HIF-2α decreased gradually, being lower by 25.3% (p < 0.05) in S3. Fpn decreased between S1 and S2 by 18.9% (p < 0.01) and remained lower during S3 (p < 0.01). In the NORM group, in turn, hepcidin levels increased gradually, being higher by 73.9% (p < 0.05) in S3 compared to S1. No statistically significant differences in EPO were observed in both groups.Conclusion: Three weeks of LH-TL suppresses resting hepcidin and ERFE levels in endurance athletes. We found no association between hepcidin and ERFE after LH-TL. Probably, ERFE is not the only factor that suppresses hepcidin expression in response to moderate hypoxia, especially in later stages of hepcidin downregulation. With the cessation of hypoxia, favorable conditions for increasing the availability of iron cease.

show abstract

Section: Discussionsupporting

confidence: 59%

Hepcidin and erythroferrone response to 3 weeks of exposure to normobaric hypoxia at rest in trained cyclists

Płoszczyca,

Czuba,

Chalimoniuk

et al. 2023

Front. Physiol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…On the one hand, in combination with growth factors, they participate in muscle regeneration and repair. On the other hand, however, the local persistence of RONS sustained by infiltrated neutrophils may cause further injury by oxidatively damaging differentiating myoblasts and myotubes thus delaying the complete restoration to health [7,8].…”

Section: Introductionmentioning

confidence: 99%

Multiple Cryotherapy Attenuates Oxi-Inflammatory Response Following Skeletal Muscle Injury

Zembroń-Łacny

Morawin

Wawrzyniak-Gramacka

et al. 2020

IJERPH

Self Cite

View full text Add to dashboard Cite

The oxi-inflammatory response is part of the natural process mobilizing leukocytes and satellite cells that contribute to clearance and regeneration of damaged muscle tissue. In sports medicine, a number of post-injury recovery strategies, such as whole-body cryotherapy (WBC), are used to improve skeletal muscle regeneration often without scientific evidence of their benefits. The study was designed to assess the impact of WBC on circulating mediators of skeletal muscle regeneration. Twenty elite athletes were randomized to WBC group (3-min exposure to −120 °C, twice a day for 7 days) and control group. Blood samples were collected before the first WBC session and 1 day after the last cryotherapy exposure. WBC did not affect the indirect markers of muscle damage but significantly reduced the generation of reactive oxygen and nitrogen species (H2O2 and NO) as well as the concentrations of serum interleukin 1β (IL-1β) and C-reactive protein (CRP). The changes in circulating growth factors, hepatocyte growth factor (HGF), insulin-like growth factor (IGF-1), platelet-derived growth factor (PDGFBB), vascular endothelial growth factor (VEGF), and brain-derived neurotrophic factor (BDNF), were also reduced by WBC exposure. The study demonstrated that WBC attenuates the cascade of injury–repair–regeneration of skeletal muscles whereby it may delay skeletal muscle regeneration.

show abstract

“…If the homogeneity and normality assumptions were violated, the Kruskal-Wallis non-parametric test was used. Additionally, eta-squared (η 2 ) was used as a measure of effect size, which is indicated as having no effect if 0 ≤ η 2 < 0.05, a small effect if 0.05 ≤ η 2 < 0.26, a moderate effect if 0.026 ≤ η 2 <0.64 and a large effect if η 2 ≥ 0.64 according to our previous study [20]. The ideal sample size for a 30-person group was n = 28, whereas for a 20-person it was set at n = 20 to demonstrate significant differences (confidence level 95% and margin error 5%).…”

Section: Discussionmentioning

confidence: 99%

Dipeptide Extract Modulates the Oxi-Antioxidant Response to Intense Physical Exercise

et al. 2022

Self Cite

View full text Add to dashboard Cite

Exposure to intense physical exercise increases reactive oxygen and nitrogen species production. The process can be modulated by dipeptide bioavailability with antioxidant scavenger properties. The effects of dipeptide intake in combination with physical exercise on the oxi-antioxidant response were examined in a randomized and placebo-controlled trial. Blood samples were collected from 20 males aged 21.2 ± 1.8 years before and after 14-day intake of chicken breast extract (4 g/day), which is a good source of bioactive dipeptides. A significant increase in the NO/H2O2 ratio was observed in the 1st and 30th minute after intense incremental exercise in dipeptides compared to the placebo group. Total antioxidant and thiol redox status were significantly higher in the dipeptide group both before and after exercise; η2 ≥ 0.64 showed a large effect of dipeptides on antioxidant and glutathione status. The level of 8-isoprostanes, markers of oxidative damage, did not change under the influence of dipeptides. By contrast, reduced C-reactive protein levels were found during the post-exercise period in the dipeptide group, which indicates the anti-inflammatory properties of dipeptides. High pre-exercise dipeptide intake enhances antioxidant status and thus reduces the oxi-inflammatory response to intense exercise. Therefore, the application of dipeptides seems to have favourable potential for modulating oxidative stress and inflammation in physically active individuals following a strenuous exercise schedule.

show abstract

Intermittent Hypoxic Exposure with High Dose of Arginine Impact on Circulating Mediators of Tissue Regeneration

Cited by 4 publications

References 54 publications

Hepcidin and erythroferrone response to 3 weeks of exposure to normobaric hypoxia at rest in trained cyclists

Hepcidin and erythroferrone response to 3 weeks of exposure to normobaric hypoxia at rest in trained cyclists

Multiple Cryotherapy Attenuates Oxi-Inflammatory Response Following Skeletal Muscle Injury

Dipeptide Extract Modulates the Oxi-Antioxidant Response to Intense Physical Exercise

Contact Info

Product

Resources

About