Internal telomeric repeats and ‘TCP domain’ protein‐binding sites co‐operate to regulate gene expression in Arabidopsis thaliana cycling cells

Abstract

SummaryWe have focused our interest on two cis-regulatory elements, named site II motif and telo box, identi®ed within the promoter of plant proliferating cellular nuclear antigen (PCNA) and putatively involved in meristematic expression of the gene. A conserved topological association between site II motifs and telo boxes is observed in the promoter of numerous genes expressed in cycling cells, including several cell cyclerelated genes and 153 Arabidopsis genes encoding ribosomal proteins. Meristematic expression of a GUS reporter gene was observed in plants under the control of Arabidopsis site II motif within a minimal promoter. This expression is strongly enhanced by addition of a telo box within this chimaeric promoter. We showed by gel retardation experiments that the site II motif is a target for several DNA-binding activities present in Arabidopsis crude cell extract and can bind a transcription factor, At-TCP20, from the Teosinte branched 1, Cycloidea, PCF (TCP)-domain protein family. In yeast two-hybrid experiments, At-TCP20 appears to be a potential partner of AtPura, which was previously shown to bind telo boxes. An important consequence of this analysis is to reveal new and conserved regulatory processes concerning the regulation of plant ribosomal gene expression in cycling cells. The implication of these observations in plant-speci®c developmental pathways is discussed.

TheArabidopsis thalianaTCP transcription factors: A broadening horizon beyond development

“…128 Site II motifs bound by TCPs, such as TCP20, are observed in the promoters of numerous genes expressed in cycling/dividing cells, including several cell cycle-related genes and 153 Arabidopsis genes encoding ribosomal proteins. 129 Functioning in determining leaf pavement cell sizes and in controlling the onset of senescence. 19 Mediating nitrate foraging by Arabidopsis roots through the systemic signaling pathway.…”

RECoN: Rice Environment Coexpression Network for Systems Level Analysis of Abiotic-Stress Response

“…All the genes in drought clusters, their relative expression across the stages and their 'drought' cluster membership are provided in Supplemental Table S5. in combination with the telo-box motif (Tremousaygue et al, 2003). These motifs are co-located in the promoters of about 70% of 216 ribosomal protein genes in Arabidopsis.…”

Jasmonate Controls Leaf Growth by Repressing Cell Proliferation and the Onset of Endoreduplication while Maintaining a Potential Stand-By Mode

“…The rate of protein synthesis is key to cellular growth, and stimulation of the expression of genes encoding components of the translational apparatus occurs in cycling cells and upon the addition of auxin in plant cells (Trémousaygue et al, 2003;Gao et al, 2008). In multicellular organisms, it is debated whether the factors determining growth impose their influence on organs as a whole or whether they regulate growth and proliferation at the cellular level (Tsukaya and Beemster, 2006).…”

“…A conserved topological association between these two elements is observed in the promoter of genes expressed in cycling cells as well as in Arabidopsis genes encoding ribosomal proteins. Interestingly, teloboxes are also observed within the promoters of other plant genes expressed in late G1 such as RNRs, raising the possibility that this element could be involved in a common regulatory process that connects the expression of a set of genes at the G1/S transition (Trémousaygue et al, 2003). RNR2A is up-regulated by MeJA and is COI1 dependent.…”