Internalization of titanium dioxide nanoparticles by glial cells is given at short times and is mainly mediated by actin reorganization-dependent endocytosis

Huerta-García, Elizabeth; Márquez-Ramírez, Sandra Gissela; Ramos‐Godínez, María del Pilar; López-Saavedra, Alejandro; Herrera, Luis A.; Parra, A Flores; Alfaro-Moreno, Ernesto; Gómez, Erika Olivia; López‐Marure, Rebeca

doi:10.1016/j.neuro.2015.08.013

Cited by 41 publications

(26 citation statements)

References 49 publications

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“…Likewise, the uptake of TiO 2 NPs by glial cells was based on the mechanism of endocytosis. However, chemical immobilization of the cytoskeleton significantly reduced the entry of TiO 2 NPs into the cells, suggesting macropinocytosis as the main process of their uptake [72].…”

Section: Cellular Uptakementioning

confidence: 98%

Toxicity of metallic nanoparticles in the central nervous system

Sawicki

Czajka

Matysiak‐Kucharek

et al. 2019

Nanotechnology Reviews

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Metallic nanoparticles due to their small size and unique physico-chemical characteristics have found excellent applications in various branches of industry and medicine. Therefore, for many years a growing interest has been observed among the scientific community in the improvement of our understanding of the impact of nanoparticles on the living organisms, especially on humans. Considering the delicate structure of the central nervous systemit is one of the organs most vulnerable to the adverse effects of metallic nanoparticles. For that reason, it is important to identify the modes of exposure and understand the mechanisms of the effect of nanoparticles on neuronal tissue. In this review, an attempt is undertaken to present current knowledge about metallic nanoparticles neurotoxicity based on the selected scientific publications. The route of entry of nanoparticles is described, as well as their distribution, penetration through the cell membrane and the blood-brain barrier. In addition, a study on the neurotoxicity in vitro and in vivo is presented, as well as some of the mechanisms that may be responsible for the negative effects of metallic nanoparticles on the central nervous system. Graphical abstract: This review summarizes the current knowledge on the toxicity of metallic NPs in the brain and central nervous system of the higher vertebrates.

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Section: Cellular Uptakementioning

confidence: 98%

Toxicity of metallic nanoparticles in the central nervous system

Sawicki

Czajka

Matysiak‐Kucharek

et al. 2019

Nanotechnology Reviews

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“…cell dead or decreased cellular activity) effect (Huerta-García et al 2015). A variety of NPs have thus been demonstrated to be internalized by osteoblasts, such as calcium phosphate nano shells (Schmidt et al 2008), implants´wear debris (Lohmann et al 2000), quantum dot/hydroxyapatite composites (Hsieh et al 2009), polymeric particles (Tautzenberger et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Internalization of (bis)phosphonate-modified cellulose nanocrystals by human osteoblast cells

et al. 2017

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Covalent conjugation of (bis)phosphonate group-containing molecules, sodium Alendronate (Aln) and 3-AminoropylPhosphoric Acid (ApA), to Cellulose nanocrystals (CNCs) was performed via oxidation/Shiff-base reaction. Further fluorescent labelling with Rhodamine B Iso ThioCyanate (RBITC) was performed to follow CNCs interaction and potential internalization with/in human osteoblasts by confocal microscopy. Complementary analyses were applied to identify the conjugation (Atenuated Total Reflectance-Fourier Transform Infrared and UV-VIS spectroscopies), physico-chemical (Dynamic Light Scattering and Nanoparticle Tracking Analysis) and morphological (Transmission Electron Microscopy) features of native and ApA/Aln-modified CNCs in physiologically relevant environments (Phosphate Buffer Saline, Advanced Dulbecco's Modified Eagle Medium). While conjugation did not affect the CNCs' size, the RBITC-labelling promotes their aggregation. Faster (1 h vs. 2 h) uptake by osteoblasts of RBITCCNCoxAln, compared to RBITC-CNCoxApA, and no-internalization (in 24 h) of native RBITTC-CNC, indicate a higher affinity of Aln-modified CNCs to the cells, while all CNCs (in 0.25-0.06 wt%) promote the cell growth. Aln/Apa-modified CNCs shows high potential in drug-delivery for bone therapies, and theranostics.

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“…It is noteworthy that, for all systems, this parameter showed maximum stability at 180 min and fluctuated at other time intervals. It has been reported [46] that internalization of agglomerates to cells in in vitro systems happens within 180 min. It is noteworthy that increments in hydrodynamic diameter of agglomerates in F12K and F12K + FBS at 180 min were related to decrements.…”

Section: Resultsmentioning

confidence: 99%

“…In the cited work, media compounds seem to form a protein corona around TiO 2 NP agglomerates that modified its surface charge and morphology. Plasma and albumin are the major components of protein corona and its formation also depends on the affinity of the TiO 2 NP for the components of the media [46]. Nonetheless, little is known on the effect of particle physicochemical properties and the mechanisms of gastrointestinal particle uptake [65].…”

Section: Fractal Dimensionmentioning

confidence: 99%

Morphological and Physicochemical Characterization of Agglomerates of Titanium Dioxide Nanoparticles in Cell Culture Media

Freyre-Fonseca

Téllez‐Medina

Medina‐Reyes

et al. 2016

Journal of Nanomaterials

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Titanium dioxide nanoparticles (TiO2NP) are possible carcinogenic materials (2B-IARC) and their toxicity depends on shape, size, and electrical charge of primary NP and on the system formed by NP media. The aim of this work was to characterize agglomerates of three TiO2NP by evaluating their morphometry, stability, and zeta potential (ζ) in liquid media and their changes with time. Sizes of agglomerates by dynamic light scattering (DLS) resulted to be 10–50 times larger than those obtained by digital image analysis (DIA) given the charged zone around particles. Fractal dimension (FD) was highest for agglomerates of spheres and belts in F12K, and in E171 in FBS media. E171 and belts increased FD with time. At time zero, using water as dispersant FD was larger for agglomerates of spheres than for of E171. Belts suspended in water had the smallest values of circularity (Ci) which was approximately unchanged with time. All dispersions hadζvalues around −30 mV at physiological pH (7.4) and dispersions of NP in water and FBS showed maximum stability (Turbiscan Lab analysis). Results help in understanding the complex NP geometry-size-stability relationships when performingin vivoandin vitroenvironmental-toxicity works and help in supporting decisions on the usage of TiO2NP.

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Internalization of titanium dioxide nanoparticles by glial cells is given at short times and is mainly mediated by actin reorganization-dependent endocytosis

Cited by 41 publications

References 49 publications

Toxicity of metallic nanoparticles in the central nervous system

Toxicity of metallic nanoparticles in the central nervous system

Internalization of (bis)phosphonate-modified cellulose nanocrystals by human osteoblast cells

Morphological and Physicochemical Characterization of Agglomerates of Titanium Dioxide Nanoparticles in Cell Culture Media

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