Internalization, reduced growth, and behavioral effects following exposure to micro and nano tire particles in two estuarine indicator species

Siddiqui, Samreen; Dickens, John; Cunningham, Brittany; Hutton, Sara J.; Pedersen, Emily; Harper, Barbara; Harper, Stacey L.; Brander, Susanne M.

doi:10.1016/j.chemosphere.2022.133934

Cited by 44 publications

(24 citation statements)

References 113 publications

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“…Americamysis bahia larvae were purchased from Aquatic Biosystems in Fort Collins, Colorado, and reared in three tanks to adulthood at 15, 20, and 25 PSU salinities with filtered artificial seawater prepared (AFSW). Following EPA protocol 833-C-09-001 (USEPA, 2009;Siddiqui et al, 2022). when adult A. bahia reproduced, larvae were moved to additional tanks of the same salinity and reared for seven days.…”

Section: Model Organisms Their Sources and Experimental Setupmentioning

confidence: 99%

“…Microfiber exposures with mysids were initiated at seven days post fertilization (dpf) (n=9) under static renewal conditions for seven days. Menidia beryllina embryos were harvested from broodstock held at the OSU Hatfield Marine Science Center and placed into three acclimation aquaria of 5, 15, and 25 PSU salinities with filtered AFSW following modified methods from Middaugh et al (1987), as done in previous studies in the Brander lab (DeCourten et al, 2020;Hutton et al, 2021;Siddiqui et al, 2022). Larvae were placed into exposure vessels at 6 ± 1 days post fertilization (dpf) (n=6) and maintained under static renewal conditions for 96 h.…”

Section: Model Organisms Their Sources and Experimental Setupmentioning

confidence: 99%

“…Following microfiber exposures of 7d (A. bahia) and 96 h (M. beryllina), behavioral assays were performed post-exposure for each treatment using a DanioVision Observation Chamber (Noldus, Wageningen, the Netherlands) for the Dark: Light cycle as described previously (Mundy et al, 2021;Segarra et al, 2021;Siddiqui et al, 2022). Briefly, A. bahia and M. beryllina larvae were randomized and placed in individual 10 ml glass beaker in a 12 well plate frame in the Ethovision Observation Chamber (EOB) to observe natural photo motor response.…”

Section: Behavioral Assaysmentioning

confidence: 99%

“…Behavior and activity were recorded and tracked by a Basler Gen 1 Camera using Ethovision XT15 software. Velocity thresholds were determined for swimming parameters between 0.5 cm/s (freezing) -2.0 cm/s (moving) (Segarra et al, 2021;Siddiqui et al, 2022). A virtual center zone (1.6 cm diameter) was established to measure the time that larvae spent in the center of the 2.2 cm diameter in the beaker.…”

Section: Behavioral Assaysmentioning

confidence: 99%

“…Per Siddiqui et al (2022), at least three individuals from each species per replicates were collected for growth. Length and width measurements were collected via dissecting scope equipped with Moticam visual software, and particle uptake was visualized on a Zeiss Axio Observer inverted microscope (Carl Zeiss, White Plains, NY).…”

Section: Growth and Microfibers Internalizationmentioning

confidence: 99%

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Natural and synthetic microfibers alter growth and behavior in early life stages of estuarine organisms

Siddiqui

Hutton²,

Dickens³

et al. 2023

Front. Mar. Sci.

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Increasing shares of microfibers are being detected in environmental samples and a closer look to identify the risk associated with them using ecologically relevant endpoints, especially at sensitive early life stages, is needed. To assess exposure hazards, we used rope samples representative of fiber types ubiquitous in coastal systems, where microfibers are often the most common debris type found in the water column. To compare responses to natural vs. synthetic microfibers, we used rinsed “natural” cotton, polyester, and polypropylene microfibers (80-150 µm length, 8-20 µm width) created from the rope. Larval and juvenile estuarine indicator species Inland Silverside (Menidia beryllina) and mysid shrimp (Americamysis bahia), respectively, were exposed to these three microfiber types at three concentrations (3, 10, 30 particles/ml) along a 5-25 PSU salinity gradient to mimic estuarine conditions. Behavioral responses, growth, and ingestion were measured. The cotton microfibers were not detected in the digestive tracts of Silversides, however, both the polyester and polypropylene microfibers were detected in the Silversides’ stomach and gut lining. None of the fiber types were detected in mysid shrimps. Mysids exposed to cotton microfibers had fewer behavioral effects compared to Silversides, who responded more to cotton. Cotton exerted no effect on growth in Silversides but did cause reduced growth in the mysids at the two lower salinities. In contrast, polyester and polypropylene were identified to have a significant dose dependent effect on mysid and Silverside behavior as well as growth was affected in at least one of the three salinities at concentrations as low as 3 particles/ml. Cotton impacted both the organism’s behavior more at higher salinities, whereas polyester and polypropylene had more impacts at lower salinities. This raises concerns for microfiber impacts on estuarine ecosystems and the need for policies to limit microfiber production and outfall into the aquatic environment.

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Section: Model Organisms Their Sources and Experimental Setupmentioning

confidence: 99%

Section: Model Organisms Their Sources and Experimental Setupmentioning

confidence: 99%