Interphase Nuclear Structure and Heterochromatin in Two Species of Corchorus and Their F1 Hybrid.

Samad, Md. Abdus; Kabir, Golam; Islam, A. S.

doi:10.1508/cytologia.57.21

Cited by 17 publications

(2 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The reduced genome sizes observed in the studied species may have also a phylogenetic signal. The clade Byttnerioideae (which includes Theobroma and Herrania ) presents small genomes (~0.43 pg), as well as it is related to the Grewioideae (~0.48 pg) clade [ 72 – 74 ]. Byttnerioideae and Grewioideae form the most basal group of Malvaceae, suggesting that the small genomes are a plesiomorphyc condition in the family.…”

Section: Discussionmentioning

confidence: 99%

Genome size, cytogenetic data and transferability of EST-SSRs markers in wild and cultivated species of the genus Theobroma L. (Byttnerioideae, Malvaceae)

et al. 2017

View full text Add to dashboard Cite

The genus Theobroma comprises several trees species native to the Amazon. Theobroma cacao L. plays a key economic role mainly in the chocolate industry. Both cultivated and wild forms are described within the genus. Variations in genome size and chromosome number have been used for prediction purposes including the frequency of interspecific hybridization or inference about evolutionary relationships. In this study, the nuclear DNA content, karyotype and genetic diversity using functional microsatellites (EST-SSR) of seven Theobroma species were characterized. The nuclear content of DNA for all analyzed Theobroma species was 1C = ~ 0.46 pg. These species presented 2n = 20 with small chromosomes and only one pair of terminal heterochromatic bands positively stained (CMA+/DAPI− bands). The small size of Theobroma ssp. genomes was equivalent to other Byttnerioideae species, suggesting that the basal lineage of Malvaceae have smaller genomes and that there was an expansion of 2C values in the more specialized family clades. A set of 20 EST-SSR primers were characterized for related species of Theobroma, in which 12 loci were polymorphic. The polymorphism information content (PIC) ranged from 0.23 to 0.65, indicating a high level of information per locus. Combined results of flow cytometry, cytogenetic data and EST-SSRs markers will contribute to better describe the species and infer about the evolutionary relationships among Theobroma species. In addition, the importance of a core collection for conservation purposes is highlighted.

show abstract

Section: Discussionmentioning

confidence: 99%

Genome size, cytogenetic data and transferability of EST-SSRs markers in wild and cultivated species of the genus Theobroma L. (Byttnerioideae, Malvaceae)

et al. 2017

View full text Add to dashboard Cite

show abstract

“…Serial dilutions of heterogeneous PCR products were used as standards against total jute genomic DNA which is approximately 1,250 Mb in length. 19 Based on signal intensities copy numbers of RT genes in Ty1-copia and Ty3-gypsy elements of jute were estimated to be approximately 31,298 and 3,621 respectively per diploid jute genome (Fig. 6).…”

Section: Resultsmentioning

confidence: 99%