Interphase Nucleolar Organizer Regions in Cancer Cells

Derenzini, Massimo; Ploton, Dominique

doi:10.1016/b978-0-12-364932-4.50008-3

Cited by 146 publications

(91 citation statements)

References 115 publications

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“…It may suggest that growing TVTs present a morphological and distribution pattern of AgNORs different from that showed by regressive TVTs. Additionally, growing TVTs have an AgNOR pattern similar to malignant tumors (Underwood and Giri, 1988;Derenzini and Ploton, 1991;Trerè, 1993;Rüschoff et al, 1994;Löhr et al, 1997;Santos et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

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Cell proliferation markers in the transplanted canine transmissible venereal tumor

Santos

Moro

Cassali

et al. 2011

Arq. Bras. Med. Vet. Zootec.

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Adult male mongrel dogs were subcutaneously transplanted with the canine transmissible venereal tumor (TVT) on the hypogastric region. Twelve specimens of tumors were collected, half during the proliferative phase and the other half during the regressive phase. Fragments of the tumor were fixed in 10% buffered formalin and routinely processed for light microscopy. Sections of 4µm were stained by Schorr or AgNOR or either immunostained for MIB1 (Ki67). Schorr stain, AgNOR and MIB1 showed an increased proliferative activity through mitotic index, nuclear argyrophilic protein stain and cycling tumoral cells in the growing tumors, respectively. All of the three cell proliferation markers were able to distinguish the TVT in both evolution phases. MIB1 monoclonal antibody was the best in the morphologic evaluation of growth and regression of TVT. This resulted in higher values than AgNORs counting and mitotic index. MIB1 immunostaining was the most effective parameter of the proliferative activity of TVT. However, a significant correlation has been detected only between mitosis counting and AgNORs. Keywords: dog, venereal tumors, MIB1, AgNOR RESUMO Cães machos, adultos, mestiços, foram transplantados com células do tumor venéreo transmissível canino (TVTC), na região hipogástrica. Foram coletados doze espécimes do TVTC

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Section: Discussionmentioning

confidence: 99%

“…AgNORs technique may be also used to estimate the cellular cycle length. The shorter the cellular cycle the more ribosomes and proteins can be produced and more AgNORs per unit of time are presented (Derenzini and Ploton, 1991;Trerè, 1993;Rüschoff et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

Cell proliferation markers in the transplanted canine transmissible venereal tumor

Santos

Moro

Cassali

et al. 2011

Arq. Bras. Med. Vet. Zootec.

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“…In human cancer cells, it has been demonstrated that Ag-NOR staining of nucleoli varies depending on cell proliferation (3,4,13) in relation to the fraction of cells in S-phase (12). This variability is estimated by image analysis of general staining of the nucleoli.…”

Section: Interest Of Ag-nor Protein Quanti-cationmentioning

confidence: 99%

“…In particular, because it has been proposed that the amount of Ag-NOR proteins is correlated with the duration of the cell cycle (3,17), it was suggested to be a prognostic factor for cancers. Finally, the quantification of Ag-NOR proteins is already included in the series of tests that are performed in hospitals to characterize human cancers.…”

Section: Introductionmentioning

confidence: 99%

Quantification of Ag-NOR proteins using Ag-NOR staining on western blots.

Roussel

Sirri²,

Hernandez‐Verdun³

1994

J Histochem Cytochem.

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Ribosomal genes are associated with a set of silver-stained nucleolar proteins, the Ag-NOR proteins, whose amount is directly related to the duration of the cell cycle. Quantification of Ag-NOR proteins by image analysis is presently used to evaluate the rate of proliferation of cancer cells and nucleolar activity. Our objective was to establish a procedure to quantdy independently each major Ag-NOR protein in cell extracts. Computerized densitometry established that the specific silver staining of Ag-NOR proteins (Ag-NOR staining) performed on Western blots makes it possible to quantdy AgNOR proteins. Using putified Ag-NOR proteins, nucleolin, and protein B23, we observed that the intensity of Ag-NOR staining is proportional to the amount of protein. A linear relationship exists between the intensity of Ag-NOR staining and the amount of nucleolin, in the range

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“…Image analysis can provide many more descriptive parameters such as the total Ag-NOR area/nucleus, the mean area/Ag-NOR dots, and the number of Ag-NORs/nucleus ( 19). However, even with quantitative indices of Ag-NOR morphology, the relationship to cellular proliferation capacity, grading, and prognosis is controversial (6,7,16).…”

mentioning

confidence: 99%

Combined light and fluorescent microscopical imaging of nucleolar organizer regions and cellular rRNA as detected by fluorescent in situ hybridization

Pajor

Honeyman

1995

Cytometry

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A method for combined light and fluorescent microscopic imaging of nucleolar organizer regions and cellular rRNA is described. Nucleolar organizer regions were detected by silver staining (Ag-NOR), and rRNA was detected by fluorescent in situ hybridization (FISH). MG-63 human fibrosarcoma cells were silver stained prior to in situ hybridization. To quantitate Ag-NOR within individual cells, brightfield images were digitized, and the total Ag-NOR arednucleus was determined. Fluorescent images were digitized, and the total cellular fluorescence was calculated after correction for nonuniformity of illumination. By using this method, it was shown that the Ag-NOR procedure did not signiticantly affect the fluorescence intensity related to FISH. Furthermore, the hybridization procedure did not interfere with quantitation of Ag-NOR. With this method, both Ag-NOR and rRNA product can be quantitated within the same cell. Because the relationship of rRNA content to cell proliferation is well established, correlation to quantitative Ag-NOR parameters within individual cells will contribute to the better definition of the relationship of quantitative Ag-NOR indices with cellular proliferation. 0 1995 Wiley-Liss, Inc.

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Interphase Nucleolar Organizer Regions in Cancer Cells

Cited by 146 publications

References 115 publications

Cell proliferation markers in the transplanted canine transmissible venereal tumor

Cell proliferation markers in the transplanted canine transmissible venereal tumor

Quantification of Ag-NOR proteins using Ag-NOR staining on western blots.

Combined light and fluorescent microscopical imaging of nucleolar organizer regions and cellular rRNA as detected by fluorescent in situ hybridization

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