Interplay between abiotic and microbial biofilm‐mediated processes for travertine formation: Insights from a thermal spring (<i>Piscine Carletti</i>, Viterbo, Italy)

Venturi, Stefanía; Crognale, Simona; Benedetto, Francesco Di; Montegrossi, Giordano; Casentini, Barbara; Amalfitano, Stefano; Baroni, Tommaso; Rossetti, Simona; Tassi, Franco; Capecchiacci, Francesco; Vaselli, Orlando; Fazi, Stefano

doi:10.1111/gbi.12516

Cited by 3 publications

(1 citation statement)

References 118 publications

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“…The hybridized bacterial cells were excited with the 488 nm line of an Ar laser (excitation) and observed in the green channel from 500 to 530 nm (emission). Precipitated minerals were visualized by their reflection signal (405 nm line of a diode laser) and appear of gray color (Venturi et al, 2022 ). The three-dimensional reconstruction of CSLM images was elaborated by the software IMARIS 7.6 (Bitplane, Switzerland) with 3D volume rendering mode.…”

Section: Methodsmentioning

confidence: 99%

Carbonate precipitation and phosphate trapping by microbialite isolates from an alkaline insular lake (Bagno dell'Acqua, Pantelleria Island, Italy)

Mazzoni,

Piacentini,

Di Bella

et al. 2024

Front. Microbiol.

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The Bagno dell'Acqua lake is characterized by CO2 emissions, alkaline waters (pH = 9) and Eh values which indicate strongly oxidizing conditions. A typical feature of the lake is the presence of actively growing microbialites rich in calcium carbonates and silica precipitates. Mineralogy, petrography and morphology analyses of the microbialites were coupled with the analysis of the microbial community, combining molecular and cultivation approaches. The DNA sequencing revealed distinct patterns of microbial diversity, showing pronounced differences between emerged and submerged microbialite, with the upper layer of emerged samples exhibiting the most distinctive composition, both in terms of prokaryotes and eukaryotes. In particular, the most representative phyla in the microbial community were Proteobacteria, Actinobacteriota, and Bacteroidota, while Cyanobacteria were present only with an average of 5%, with the highest concentration in the submerged intermediate layer (12%). The role of microorganisms in carbonate mineral formation was clearly demonstrated as most of the isolates were able to precipitate calcium carbonate and five of them were characterized at molecular level. Interestingly, when microbial isolates were cultivated only in filtered water, the precipitation of hazenite was observed (up to 85%), opening new prospective in P (phosphate) recovery from P depleted environments.

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Section: Methodsmentioning

confidence: 99%

Carbonate precipitation and phosphate trapping by microbialite isolates from an alkaline insular lake (Bagno dell'Acqua, Pantelleria Island, Italy)

Mazzoni,

Piacentini,

Di Bella

et al. 2024

Front. Microbiol.

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Microbial Diversity and Activity of Biofilms from Geothermal Springs in Croatia

et al. 2023

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Hot spring biofilms are stable, highly complex microbial structures. They form at dynamic redox and light gradients and are composed of microorganisms adapted to the extreme temperatures and fluctuating geochemical conditions of geothermal environments. In Croatia, a large number of poorly investigated geothermal springs host biofilm communities. Here, we investigated the microbial community composition of biofilms collected over several seasons at 12 geothermal springs and wells. We found biofilm microbial communities to be temporally stable and highly dominated by Cyanobacteria in all but one high-temperature sampling site (Bizovac well). Of the physiochemical parameters recorded, temperature had the strongest influence on biofilm microbial community composition. Besides Cyanobacteria, the biofilms were mainly inhabited by Chloroflexota, Gammaproteobacteria, and Bacteroidota. In a series of incubations with Cyanobacteria-dominated biofilms from Tuhelj spring and Chloroflexota- and Pseudomonadota-dominated biofilms from Bizovac well, we stimulated either chemoorganotrophic or chemolithotrophic community members, to determine the fraction of microorganisms dependent on organic carbon (in situ predominantly produced via photosynthesis) versus energy derived from geochemical redox gradients (here simulated by addition of thiosulfate). We found surprisingly similar levels of activity in response to all substrates in these two distinct biofilm communities, and observed microbial community composition and hot spring geochemistry to be poor predictors of microbial activity in the study systems.

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Experimental vs. Natural Mineral Precipitation in Modern Microbialites: The Case Study of the Alkaline Bagno Dell’acqua Lake (Pantelleria Island, Italy)

Ingrassia,

Conte,

Perinelli

et al. 2024

Minerals

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Microbial activity has been documented in various lacustrine environments, suggesting its fundamental role in mineral precipitation and, therefore, in the formation of organo-deposits such as microbialites. Many studies are currently focused on documenting how the association of microbes and extracellular polymeric substances (EPSs) may influence the authigenesis of Mg-rich clay minerals and the subsequent carbonate precipitation in growing microbialites in lacustrine environments. In this study, we investigate the present-day microbialites of the alkaline Bagno dell’Acqua lake (Pantelleria Island, Italy) using X-ray diffraction (XRD) scanning electron microscopy (SEM) and energy-dispersive X-ray spectroscopy (EDS). Our results reveal the intimate association of Mg-smectite/carbonate minerals with the EPS and microbes, and, for the first time, we selected microbes belonging to phylum Firmicutes (Bacillus sp.), from natural microbialites, to carry out laboratory experiments that testify their direct role in the precipitation of clay and carbonate minerals.

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Interplay between abiotic and microbial biofilm‐mediated processes for travertine formation: Insights from a thermal spring (Piscine Carletti, Viterbo, Italy)

Cited by 3 publications

References 118 publications

Carbonate precipitation and phosphate trapping by microbialite isolates from an alkaline insular lake (Bagno dell'Acqua, Pantelleria Island, Italy)

Carbonate precipitation and phosphate trapping by microbialite isolates from an alkaline insular lake (Bagno dell'Acqua, Pantelleria Island, Italy)

Microbial Diversity and Activity of Biofilms from Geothermal Springs in Croatia

Experimental vs. Natural Mineral Precipitation in Modern Microbialites: The Case Study of the Alkaline Bagno Dell’acqua Lake (Pantelleria Island, Italy)

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