Interplay between hereditary and environmental factors to establish an in vitro disease model of keratoconus

Roy, Subhadeep; Yadav, Saumya; Dasgupta, Tanushree; Chawla, Smriti; Tandon, Radhika; Ghosh, Sourabh

doi:10.1016/j.drudis.2018.10.017

Cited by 13 publications

(11 citation statements)

References 116 publications

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“…In view of no determined evaluation criteria for the success of corneal ectasia animal model [7] , we focused on increment in diopter changes, reduction in corneal thickness and biomechanical related parameters, which are regard as the signs of cornea ectasia. Keratometer (TOPCON, Japan) and Optical Coherence Tomography (OCT, TOPCON, Japan) were used to get corneal diopter along horizontal and vertical meridians (D_H and D_V).…”

Section: Morphology and Biomechanical Related Parametersmentioning

confidence: 99%

“…An animal model with corneal anatomy and physiology similar to human being is a valuable and indispensable tool in basic researches. Genetic approach, corneal stromal ablation, and enzyme have been applied to construct corneal ectasia animal models [7][8][9][10][11][12][13][14][15] . Tachibana et al [8] constructed spontaneous mutated mice with keratoconus appearance, which are more suitable for genetic and molecular researches.…”

Section: Introductionmentioning

confidence: 99%

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Biomechanical and morphological changes of rabbit corneas under collagenase type II and negative pressure: three months follow-up observation

Chen

Xiao

et al. 2020

Preprint

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Abstract Background To investigate biomechanical and morphological changes of rabbit cornea ectasia induced by collagenase type II and negative pressure during 3 months after treatment. Method Eighteen New Zealand white rabbits were randomly and evenly arranged into three groups. The left corneas were continuously treated by negative pressure suction (NP group) with 500 mmHg for 30 min once two days, three times in total. The central area of left corneas were soaked in the collagenase type II (CII group) solution (200 μL of 3 mg/ml) for 30 min. The left corneas (CP group) were disposed as CII group firstly, then applied negative pressure suction as NP group for once after 5 days. All right corneas were treated as control eyes. Corneal morphology parameters and Ocular Response Analyzer (ORA) output parameters were collected in vivo once a week for three weeks after treatment and before execution. Histology and biomechanics were tested in vitro at the third month after treatment. Paired t-test and repeated measures analysis were used to determine if there were differences in biomechanical and morphological related parameters across time. Results In NP group, corneal thickness and diopter changed to some extent after treatment immediately, and the elastic modulus increased and relaxation degree slowed after 3 months. In CII group, corneal diopter increased, corneal central thickness (CCT) and corneal hysteresis (CH) decreased at the second week after treatment, which showed the characters of ectatic corneas. Then the degree of ectasia decreased with time. No regular changes was found on experimental corneas in CP group. Conclusions Collagenase type II results in ectatic corneas around two weeks after treatment, but the degree of ectasia decreased with time, and there was no significant difference compared with the controls after 3 months. After negative pressure suction, corneal morphology changed in a short period, and elastic modulus increased and relaxation time increased after a three months recovery, indicating that the negative pressure suction do have a certain effect on corneas.

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Section: Morphology and Biomechanical Related Parametersmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Biomechanical and morphological changes of rabbit corneas under collagenase type II and negative pressure: three months follow-up observation

Chen

Xiao

et al. 2020

Preprint

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“…1,2 The onset of the condition is common in the first or second decade of life, progressing until the third or fourth decade before regaining stability. 3,4 The extent of the progression depends on the interplay between the environmental context, genetic factors, time of onset as well as diagnostic and intervention methods used. 5,6 The prevalence of KC has been reported in multiple countries, including the United States (500 cases per 100,000 people), Japan (9 per 100,000 people), India (2300 per 100,000 people), Israel (2340 per 100,000 people), Macedonia (7 per 100,000 people), Iran (2400 per 100,000 people), the Netherlands (13,300 per 100 000 people), China (27 per 100,000 people) and Egypt (170 per 100 000).…”

Section: Introductionmentioning

confidence: 99%

“…Although the actual cause of keratoconus remains unknown, studies to establish the etiology investigated family history, ethnicity and environmental precipitating factors such as high temperatures and food allergies. 4,11 Cited risk factors include individual gene composition, ultraviolet and sun exposure, eye rubbing, gender, hormonal variation, age, atopy, floppy eyelid syndrome and parental education. 4,6,12 In addition, connective tissue disorders such as Ehler-Danlos syndrome, Marfan syndrome, Lonstein disease, Down syndrome and mitral valve prolapse are the most common associated disease conditions.…”

Section: Introductionmentioning

confidence: 99%

“…4,11 Cited risk factors include individual gene composition, ultraviolet and sun exposure, eye rubbing, gender, hormonal variation, age, atopy, floppy eyelid syndrome and parental education. 4,6,12 In addition, connective tissue disorders such as Ehler-Danlos syndrome, Marfan syndrome, Lonstein disease, Down syndrome and mitral valve prolapse are the most common associated disease conditions. 6 There is a huge disparity in access to eye care services, diagnostic equipment and management options between low-and high-income countries.…”

Section: Introductionmentioning

confidence: 99%

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<p>Visual Acuity and Refractive Error Improvement in Keratoconic Patients: A Low-Income Context Management Perspective</p>

Yego

Moodley

2020

OPTO

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Introduction: Visual acuity (VA) and refractive error (RE) remain important parameters in the management of keratoconic (KC) patients. Despite rapid amelioration in capacity of diagnostic equipment, these remain inaccessible to the majority of practitioners in lowincome countries. Notwithstanding limitations, practitioners are expected to utilize existing resources to satisfactorily manage their increasing numbers of keratoconic patients. Purpose: To determine the changes in visual acuity and refractive errors with diagnostic and management options available in low-income contexts. Methods: A descriptive, retrospective chart analysis of medical records dating back 5 years was employed in this study. Records of patients prescribed with corneal rigid gas permeable (RGP) and scleral lenses were analyzed. Data on age, gender, stage of keratoconus, pre-, and post-visual acuities and refractive errors, mode of correction and lens parameters were evaluated. Results: A total of 124 medical records were analyzed. Males comprised 58.9% and females 41.1% of the total sample, with a mean age 20.86 ± 9.50 years. The mean unaided VA in logMAR notation was 1.0±0.19, while corrected VA was 0.18±0.17. There was a significant improvement (p=0.001) in VA with corneal RGP contact lenses (mean 0.19±0.17) as compared to unaided VA (mean 1.29±0.20). Scleral lens VA also improved from a median of 1.06 to −0.01 logMAR; p=0.001. The mean RE before RGP contact lens fitting was −9.43 ±2.37 diopters (D) which improved to −0.41±0.78D. RE reduced significantly (p=0.001) after fitting with both corneal RGP lenses (from a mean of −9.80±4.46D to −0.45±0.80D) and scleral lenses (from a median of −8.00D to −0.02D). Conclusion: Significantly improved visual acuity and refractive error status were achieved with all KC patients. Despite the diagnostic equipment and contact lenses design limitations, practitioners in low-income contexts can fit the relatively more affordable corneal RGP lenses to positively impact the daily living experiences of their KC patients.

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Omics Analyses in Keratoconus: from Transcriptomics to Proteomics

Cai

Estes

Liu

2020

Curr Ophthalmol Rep

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Interplay between hereditary and environmental factors to establish an in vitro disease model of keratoconus

Cited by 13 publications

References 116 publications

Biomechanical and morphological changes of rabbit corneas under collagenase type II and negative pressure: three months follow-up observation

Biomechanical and morphological changes of rabbit corneas under collagenase type II and negative pressure: three months follow-up observation

<p>Visual Acuity and Refractive Error Improvement in Keratoconic Patients: A Low-Income Context Management Perspective</p>

Omics Analyses in Keratoconus: from Transcriptomics to Proteomics

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