Interplay Between O
            <sub>2</sub>
            and Iron in Gene Expression: Environmental Sensing by FNR, ArcA, and Fur in Bacteria

Troxell, Bryan; Hassan, Hosni M.

doi:10.1002/9781119004813.ch105

Cited by 1 publication

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“…Low oxygen tension and high osmolarity are conditions traditionally used to induce the SPI1 T3SS (Ni Bhriain et al , ; Lee and Falkow, ; Tartera and Metcalf, ; Ellermeier and Slauch, ; Jennewein et al , ). Salmonella primarily utilizes two major regulators, Fnr and the ArcAB two‐component system, to adapt to oxygen‐related environmental changes (Fink et al , ; Lim et al , ; Troxell and Hassan, ). Fnr is directly inactivated by oxygen via oxidation of its 4Fe‐4S cluster (Khoroshilova et al , ).…”

Section: Introductionmentioning

confidence: 99%

Oxygen‐dependent regulation of SPI1 type three secretion system by small RNAs in Salmonella enterica serovar Typhimurium

Kim

Golubeva

Vanderpool

et al. 2018

Molecular Microbiology

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Summary Salmonella Typhimurium induces inflammatory diarrhea and uptake into intestinal epithelial cells using the Salmonella pathogenicity island 1 (SPI1) type III secretion system (T3SS). Three AraC‐like regulators, HilD, HilC and RtsA, form a feed‐forward regulatory loop that activates transcription of hilA, encoding the activator of the T3SS structural genes. Many environmental signals and regulatory systems are integrated into this circuit to precisely regulate SPI1 expression. A subset of these regulatory factors affects translation of hilD, but the mechanisms are poorly understood. Here, we identified two sRNAs, FnrS and ArcZ, which repress hilD translation, leading to decreased production of HilA. FnrS and ArcZ are oppositely regulated in response to oxygen, one of the key environmental signals affecting expression of SPI1. Mutational analysis demonstrates that FnrS and ArcZ bind to the hilD mRNA 5′ UTR, resulting in translational repression. Deletion of fnrS led to increased HilD production under low‐aeration conditions, whereas deletion of arcZ abolished the regulatory effect on hilD translation aerobically. The fnrS arcZ double mutant has phenotypes in a mouse oral infection model consistent with increased expression of SPI1. Together, these results suggest that coordinated regulation by these two sRNAs maximizes HilD production at an intermediate level of oxygen.

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Section: Introductionmentioning

confidence: 99%

Oxygen‐dependent regulation of SPI1 type three secretion system by small RNAs in Salmonella enterica serovar Typhimurium

Kim

Golubeva

Vanderpool

et al. 2018

Molecular Microbiology

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Interplay Between O ₂ and Iron in Gene Expression: Environmental Sensing by FNR, ArcA, and Fur in Bacteria

Cited by 1 publication

References 174 publications

Oxygen‐dependent regulation of SPI1 type three secretion system by small RNAs in Salmonella enterica serovar Typhimurium

Oxygen‐dependent regulation of SPI1 type three secretion system by small RNAs in Salmonella enterica serovar Typhimurium

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Interplay Between O 2 and Iron in Gene Expression: Environmental Sensing by FNR, ArcA, and Fur in Bacteria

Cited by 1 publication

References 174 publications

Oxygen‐dependent regulation of SPI1 type three secretion system by small RNAs in Salmonella enterica serovar Typhimurium

Oxygen‐dependent regulation of SPI1 type three secretion system by small RNAs in Salmonella enterica serovar Typhimurium

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Interplay Between O ₂ and Iron in Gene Expression: Environmental Sensing by FNR, ArcA, and Fur in Bacteria