Interrelationships among morphology, echotexture, and function of the bovine corpus luteum during the estrous cycle

Siqueira, L. G. B.; Torres, Ciro Alexandre Alves; Amorim, L. S.; Souza, Emiliane Nogueira de; Camargo, L. S. A.; Fernandes, C. A. C.; Viana, J. H. M.

doi:10.1016/j.anireprosci.2008.11.009

Cited by 58 publications

(80 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Corpus luteum is a transitory gland responsible for sinthesys and release of progesterone (P 4 ), a steroidal hormone essential for early embryo development and pregnancy support (Smith et al, 1994). Correct and accurate corpus luteum identification is crucial for the success of assisted reproduction programs.…”

Section: Introductionmentioning

confidence: 99%

Luteal dynamics in goats: morphological and endocrine features

et al. 2010

View full text Add to dashboard Cite

-The aim of this study was to establish the morphologic and endocrine characteristics of luteal dynamics in goats. It was used Toggenburg female goats that showed natural estrus in a 48-hour interval. After estrus, ultrasonographic evaluations of the ovaries were daily performed during 21 days using a portable device (5MHz probe). Blood sample was collected for plasma progresterone (P 4 ) determination. Corpora lutea were detected for the first time on day 5 and progressively increased in size until D9 (1.26 ± 0.08 cm 2 ), with no variation on subsequent days. In females with one ovulation, the first visualization of the corpora lutea was earlier than in those with multiple ovulation (4.54 ± 0.18 vs 5.74 ± 0.25 days). At the moment of the first visualization, luteal area was smaller in animals with single ovulation. Plasma P 4 concentration progressively increased until day 9 and it did not show significant increase until luteolysis, characterized by a sharp decrease in P 4 concentration, reaching values below 1 ng/mL in 24 hours. The luteal area slowly and gradually decreased in size. It was observed a significant positive correlation between P 4 concentration and area during luteogenesis and luteolysis (r = 0.63 and r = 0.50, respectively).When corpus luteum reached its maximum size (D9), female with more than one corpora lutea, with a greater luteal tissue area, did not show P 4 concentration higher than those with one ovulation (5.92 ± 0.59 vs 7.04 ± 0.79 ng/mL). These results show that luteal dynamics in Toggenbur goats follow a similar pattern to those observed in other goat breeds and luteal tissue growth was positively correlated with corpora lutea functionality.Key Words: corpus luteum, goats, progesterone, ultrasonography Dinâmica luteal em caprinos: características morfológicas e endócrinas RESUMO -Objetivou-se neste estudo estabelecer as características morfológicas e endócrinas da dinâmica luteal em cabras. Foram utilizadas fêmeas da raça Toggenburg que manifestaram estro natural em um intervalo de 48 horas. Após o estro, foram realizadas avaliações ultrassonográficas diárias dos ovários durante 21 dias, utilizando-se um aparelho portátil (5 MHz).Amostras de sangue foram coletadas para dosagem de progesterona (P 4 ) no plasma. Os corpos lúteos foram detectados pela primeira vez no D5 e aumentaram progressivamente de tamanho até o D9 (1,26 ± 0,08 cm 2 ), não havendo variação nos dias subsequentes. Nas fêmeas com uma ovulação, a primeira visualização do corpo lúteo foi mais precoce que naquelas com ovulação múltipla (4,54 ± 0,18 vs 5,74 ± 0,25 dias). No momento da primeira visualização, a área luteal foi menor nos animais com uma ovulação. A concentração plasmática de P 4 aumentou progressivamente até o D9 e não apresentou aumento significativo até o momento da luteólise, caracterizada por uma acentuada queda da concentração de P 4 , atingindo valores inferiores a 1 ng/mL em um intervalo de 24 horas. A área luteal diminuiu de forma lenta e gradual. Foi observada uma correlação positiva signif...

show abstract

Section: Introductionmentioning

confidence: 99%

Luteal dynamics in goats: morphological and endocrine features

et al. 2010

View full text Add to dashboard Cite

show abstract

“…Plasma P4 concentrations gradually increase with the formation of the CL, reaching a maximum value in the mid-dioestrus phase between Day 10 to 14 of oestrous cycle (Kastelic et al, 1990, Siqueira et al, 2009. Larger variations in expression of immune genes have been observed in endometrial transcriptome profile from cows in mid-dioestrus (Day 12) compared to early metoestrus (Day 3.5) (Mitko et al, 2008).…”

Section: Discussionmentioning

confidence: 91%

63 Effect of Endometrial Biopsy on Uterine Health of Tropically Adapted Beef Cattle

Ramirez-Garzon

Satake

Lyons

et al. 2017

Reprod. Fertil. Dev.

View full text Add to dashboard Cite

In cattle, early embryonic loss during the first two to three weeks after fertilisation is recognised as the major cause of reproductive failure, with consequent significant impacts on business performance of beef and dairy farms. Inadequate endometrial receptivity is considered an important cause of early embryonic mortality in cattle. A pilot experiment was conducted to compare the outcome of performing different endometrial sampling procedures in tropically adapted (Bos indicus x Bos taurus) heifers, and to select the most suitable method to collect samples for investigation of endometrial gene expression. Endometrial biopsy (EB) using a biopsy device with a diameter similar to an artificial insemination gun (10366L Karl Storz®, Germany) was found to be the most reliable method of collecting endometrial samples in these heifers, with the samples recovered yielding a mean concentration of RNA of 72.9 ±39.3 ng/µl and mean RNA integrity number (RIN) of 7.3 ±1.1.To assess the likely safety of conducting EB in cattle a systematic review of published information assessing the impact of EB on subsequent pregnancy rates was conducted. Using the Preferred Reporting Items for Systematic reviews and Meta-Analysis (PRISMA) protocol, online searches identified 1164 possible studies. After applying the exclusion criteria, six studies allowed the comparison of pregnancy rates between sampled (n= 1,208) and non-sampled cows (n=2,393). The results showed that EB can be performed before breeding without adversely affecting the likelihood of cows becoming pregnant.A major objective of the research was to determine whether EB and evaluation of endometrial gene expression could be used to identify tropically adapted embryo transfer (ET) recipients more likely to become pregnant. Three potential EB scenarios were investigated, 1) EB on Day 7 of the oestrous cycle prior to the cycle when ET would be performed, 2) EB on Day 4 of the oestrous cycle in which ET would be performed i.e. EB 3 days before ET, and 3) EB on the same day when ET would be performed.The first study assessed the morphological and inflammatory changes induced by endometrial biopsy. Pre-synchronised heifers underwent EB from the horn ipsilateral to the corpus luteum (CL) either at Day 7 of the previous cycle (Group 1 n=10) followed by administration of a luteolytic dose of prostaglandin F2α or at Day 4 (Group 2, n=7) of the present cycle. All heifers were slaughtered on Day 7 of the present cycle. The site of EB was visually evident in 40% (4/10) of Group 1 and 42% (3/7) of Group 2 heifers. Histological assessment revealed no evidence of changes indicative of endometritis in either the biopsied or non-biopsied horns in both groups. The histological iii changes observed included mild mononuclear infiltration of the epithelium and mild vascular changes associated with haemorrhage and congestion.Initial investigation of the endometrial expression of the pro-inflammatory cytokine IL1β and wound healing cytokine TGFβ genes found no significant difference betwe...

show abstract

“…A ultrassonografia é uma metodologia mais acurada para a avaliação ovariana e possibilita não apenas a identificação, mas também a mensuração das estruturas presentes (Padula & Macmillan, 2005). Os equipamentos ultrassonográficos frequentemente utilizados não possibilitam a caracterização de folículos ovarianos em degeneração; no entanto, permitem diferenciar cistos com presença ou ausência de parede luteinizada (Siqueira et al, 2009). A eficiência, nesse caso, é limitada pela sensibilidade do equipamento e pela experiência do operador (Farin et al, 1990).…”

Section: Accuracy Of Ultrasound and Behavioral Assessment In Determinunclassified

“…Foram registrados o diâmetro médio, em dois eixos perpendiculares, da estrutura cística e a existência ou não de bordo luteinizado (cisto luteinizado) -parede folicular com espessura ≥3 mm e formada por tecido com ecogeinicidade semelhante à de corpo lúteo. Estruturas com parede folicular <3 mm foram caracterizadas como cistos foliculares (Siqueira et al, 2009). Todos os procedimentos foram realizados por um único técnico.…”

Section: Accuracy Of Ultrasound and Behavioral Assessment In Determinunclassified

Acurácia da ultrassonografia e da avaliação comportamental na determinação das características funcionais de estruturas ovarianas císticas

Palhão

Filho

Viana

et al. 2014

Pesq. agropec. bras.

View full text Add to dashboard Cite

Resumo -O objetivo deste trabalho foi avaliar a acurácia da ultrassonografia e da sintomatologia em determinar características funcionais de estruturas ovarianas císticas. Em 51 vacas Girolando sem corpo lúteo, foram avaliados: presença ou ausência de tecido luteal na parede do folículo; concentrações séricas de progesterona (P4); e sinais comportamentais dos animais (anestro ou ninfomania). As concentrações plasmáticas de P4 foram consideradas como padrão ouro. A avaliação ultrassonográfica foi um método preciso (82,4%) para diagnosticar o tipo de cisto folicular. O anestro está relacionado à presença de cisto luteinizado e à elevada P4. Os cistos não luteinizados são frequentemente encontrados em animais com baixa P4, que apresentam comportamento de anestro ou ninfomania.Termos para indexação: anestro, cisto folicular, cisto luteinizado, Girolando, ninfomania. Accuracy of ultrasound and behavioral assessment in determining functional features of ovarian cystic structuresAbstract -The objective of this work was to evaluate the accuracy of ultrasound and symptomatology in determining functional features of ovarian cystic structures. In 51 Girolando cows, without corpus luteum, the following were evaluated: presence or absence of luteinized tissue on the follicle wall; serum concentrations of progesterone (P4); and animal clinical signals (anestrus or nymphomania). P4 plasma concentrations were considered as the gold standard. Ultrasound evaluation was an accurate (82.4%) method to diagnose the type of follicular cyst. Anestrus is related to the presence of luteinized cysts and high P4 levels. Non-luteinized cysts are often found in animals with low P4, which exhibit anestrus or nymphomaniac behavior.

show abstract

Interrelationships among morphology, echotexture, and function of the bovine corpus luteum during the estrous cycle

Cited by 58 publications

References 37 publications

Luteal dynamics in goats: morphological and endocrine features

Luteal dynamics in goats: morphological and endocrine features

63 Effect of Endometrial Biopsy on Uterine Health of Tropically Adapted Beef Cattle

Acurácia da ultrassonografia e da avaliação comportamental na determinação das características funcionais de estruturas ovarianas císticas

Contact Info

Product

Resources

About