Interrelationships between Tooth Properties and Biochemical Bone Turnover Markers Investigated on Six-Month-Old Pig Model

Abstract

ABSTRACT. The aim of the study was to determine interrelationships between bone tissue metabolism indices and morphological, biomechanical and densitometric properties of hard dental tissues. First primary maxillary incisor from 6-month-old pigs (N=27) was evaluated in terms of weight and length. Mean volumetric tooth mineral density, total tooth volume, enamel total volume, enamel volumetric mineral density, dentine total volume and dentine volumetric mineral density were estimated with the use of quantitative computed tomography and micro computed tomography techniques. Tooth mineral density and tooth mineral content were evaluated with the use of dual-energy X-ray absorptiometry. Microhardness of enamel was measured using Vicker's test. Evaluations of total calcium, ionized calcium, magnesium, phosphorus, alkaline phosphatase, bone alkaline phosphatase, osteocalcin, C-terminal telopeptide of type-I collagen (CTX), insulin-like growth factor-1, growth hormone and parathyroid hormone were performed in plasma and serum samples. Pearson's correlation coefficients were determined between all the investigated variables, and P<0.05 was considered as statistically significant. The obtained results have shown mainly mutual dependences between biochemical indicators of bone metabolism. Evaluation of CTX concentration in serum of pigs has shown the highest predictive value in relation to morphological, densitometric and biomechanical properties of teeth. KEY WORDS: bone turnover marker, densitometry, quantitative computed tomography, swine, tooth.

Morphometric, densitometric and mechanical properties of mandibular deciduous teeth in 5-month-old Polish Merino sheep

“…Mean vTMD was measured for whole sample, including all the anatomical structures and reflects mean density of all hard tissues in a single tooth. For Tvol and MvTMD determinations, the volume-of-interest was defined at the values between minimum and maximum density of the investigated samples at 0 and 3071 Hounsfield units (HU), respectively [18]. …”

“…Binary images were obtained with histogram. Two regions of interest (ROI) were defined for the microtomographic images; ROI-1 for enamel and ROI-2 for dentine evaluation [18]. The volume-of-interest was defined at the values between minimum and maximum density of the investigated samples at -1000 and 3071 HU.…”

“…The second premolar tooth (p 2 ) was subjected to a compression test and ultimate force (Wf) was determined. Tooth sample was placed on the buccal (lateral) surface and the loading head compressed tooth on the lingual (medial) surface with the speed of 10 mm/min [18]. …”

Interrelationships between Morphological, Densitometric and Mechanical Properties of Eggs in Japanese Quails (<i>Coturnix Japonica</i>)

“…This study for the first time presents use of both QCT and DEXA for investigations of morphological and densitometric properties of egg in Japanese quails and their common relationships. Both these methods are commonly used for non-invasive in vivo or ex vivo determinations of quality of teeth and bones of skeletal system in mammals and birds (Charuta et al, 2008;Tatara et al, 2011;Charuta and Cooper, 2012;Tymczyna et al, 2012;Tymczyna et al, 2013). In case of both these methods, morphological imaging and densitometric measurements are based on data obtained from X-rays attenuation during passage throughout the examined object.…”

Interrelationships between Bone Metabolism Markers and Deciduous Teeth and Mandible Properties in Polish Merino Sheep

“…Bone tissue, dentine and enamel represent physiologically mineralized tissues in the animal and human body. Dentine and bone are connective tissues showing similarities in terms of chemical composition and formation processes 1) . Organic matrix of bone tissue and dentine is rich in collagen fi bers, mainly type I, and consists of a mineral in the form of hydroxyapatite crystals.…”