Interspecies somatic cell nuclear transfer: a salvage tool seeking first aid

Loi, Pasqualino; Modliński, Jacek A.; Ptak, Grazyna

doi:10.1016/j.theriogenology.2011.01.016

Cited by 83 publications

(67 citation statements)

References 48 publications

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“…We observed that interspecific blastocysts decreased the relative expression of these genes when we compared Ch1X vs Dc1X. Abnormalities in the transcription of reprogramming genes were reported in several studies in which iSCNT was performed (Loi et al 2011); these have included feline species as the marble cat (Imsoonthornruksa et al 2010) and the black-footed cat (Gó mez et al 2011). The inefficient gene expression may be related to the lower blastocyst rates and blastocyst quality obtained using Ch cells compared to Dc cells.…”

Section: Scnt and Embryo Aggregation In Felidsmentioning

confidence: 78%

Cheetah interspecific SCNT followed by embryo aggregation improves in vitro development but not pluripotent gene expression

Moro¹,

Hiriart²,

Buemo³

et al. 2015

Reproduction

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The aim of this study was to evaluate the capacity of domestic cat (Dc, Felis silvestris) oocytes to reprogram the nucleus of cheetah (Ch, Acinonyx jubatus) cells by interspecies SCNT (iSCNT), by using embryo aggregation. Dc oocytes were in vitro matured and subjected to zona pellucida free (ZP-free) SCNT or iSCNT, depending on whether the nucleus donor cell was of Dc or Ch respectively. ZP-free reconstructed embryos were then cultured in microwells individually (Dc1X and Ch1X groups) or in couples (Dc2X and Ch2X groups). Embryo aggregation improved in vitro development obtaining 27.4, 47.7, 16.7 and 28.3% of blastocyst rates in the Dc1X, Dc2X, Ch1X and Ch2X groups, respectively (P!0.05). Moreover, aggregation improved the morphological quality of blastocysts from the Dc2X over the Dc1X group. Gene expression analysis revealed that Ch1X and Ch2X blastocysts had significantly lower relative expression of OCT4, CDX2 and NANOG than the Dc1X, Dc2X and IVF control groups. The OCT4, NANOG, SOX2 and CDX2 genes were overexpressed in Dc1X blastocysts, but the relative expression of these four genes decreased in the Dc2X, reaching similar relative levels to those of Dc IVF blastocysts. In conclusion, Ch blastocysts were produced using Dc oocytes, but with lower relative expression of pluripotent and trophoblastic genes, indicating that nuclear reprogramming could be still incomplete. Despite this, embryo aggregation improved the development of Ch and Dc embryos, and normalized Dc gene expression, which suggests that this strategy could improve full-term developmental efficiency of cat and feline iSCNT embryos.Reproduction (2015) 150 1-10

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Section: Scnt and Embryo Aggregation In Felidsmentioning

confidence: 78%

Cheetah interspecific SCNT followed by embryo aggregation improves in vitro development but not pluripotent gene expression

Moro¹,

Hiriart²,

Buemo³

et al. 2015

Reproduction

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“…ISCNT is a general term, often misused because in some reports nuclear transfer is accomplished between cell and oocyte donors belonging to different families, orders, or even classes (Loi et al 2011 ).…”

Section: "Canonical" Interspecifi C Somatic Cells Nuclear Transfer (Imentioning

confidence: 99%

Cloning the Mammoth: A Complicated Task or Just a Dream?

Loi

Saragusty

Ptak

2014

Advances in Experimental Medicine and Biology

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Recently there has been growing interest in applying the most advanced embryological tools, particularly cloning, to bring extinct species back to life, with a particular focus on the woolly mammoth (Mammuthus primigenius). Mammoth's bodies found in the permafrost are relatively well preserved, with identifiable nuclei in their tissues. The purpose of this chapter is to review the literature published on the topic, and to present the strategies potentially suitable for a mammoth cloning project, with a frank assessment of their feasibility and the ethical issues involved.

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“…The only hope is then to use oocytes from closely related species. Interspecies SCNT (ISCNT), performed by injecting the nucleus from one species into the oocyte of another has also been carried out in a variety of species (for a recent review see Loi et al, 2011a). These include ISCNT from the endangered mouflon to a domestic sheep (Ovis aries) (Loi et al, 2001), from red panda (Ailurus fulgens) to rabbit (Tao et al, 2009), from sand cat (Felis margarita) to domestic cat (Gómez et al, 2008), from Canada lynx (Lynx canadensis) to both domestic cat and caracal (Caracal caracal) (Gómez et al, 2009), from water buffalo (Bubalus bubalis) to cow (Bos taurus) (Srirattana et al, 2011), and most strikingly -from a 15,000 year-old wooly mammoth (Mammuthus primigenius) to a mouse (Kato et al, 2009).…”

Section: Somatic Cells Cryopreservation For Scntmentioning

confidence: 99%

Genome Banking for Vertebrates Wildlife Conservation

Saragusty¹

2012

Current Frontiers in Cryobiology

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Interspecies somatic cell nuclear transfer: a salvage tool seeking first aid

Cited by 83 publications

References 48 publications

Cheetah interspecific SCNT followed by embryo aggregation improves in vitro development but not pluripotent gene expression

Cheetah interspecific SCNT followed by embryo aggregation improves in vitro development but not pluripotent gene expression

Cloning the Mammoth: A Complicated Task or Just a Dream?

Genome Banking for Vertebrates Wildlife Conservation

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