Interventions for men and women with their first episode of genital herpes

Heslop, Rachel; Jordan, Vanessa; Trivella, Marialena; Papastamopoulos, Vasileios; Roberts, Helen

doi:10.1002/14651858.cd010684

Cited by 2 publications

(1 citation statement)

References 19 publications

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“…After primary infection, HSV-1 and HSV-2 establish life-long persistence which may 160 result in recurrent disease which typically manifests as herpes labialis or herpes 161 genitalis and which may be associated with significant morbidity (Gnann & Whitley,162 2016; Heslop et al, 2016;Klysik et al, 2018). Even in the case of herpes labialis, which is not commonly associated with complications, treatment success is not always 164 satisfactory as indicated by the introduction of topical acyclovir/ hydrocortisone 165 combinations (Nguyen et al, 2014).…”

Section: Effects Of Omeprazole In Combination With Acyclovir On Hsv-1mentioning

confidence: 99%

Drug repurposing: omeprazole increases the efficacy of acyclovir against herpes simplex virus type 1 and 2

Michaelis

Kleinschmidt²,

Wass

et al. 2018

Preprint

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ObjectivesOmeprazole was shown to improve the anti-cancer effect of the nucleoside-analogue 5-fluorouracil. Here, we investigated the effects of omeprazole on the activities of the antiviral nucleoside analogues ribavirin and acyclovir.MethodsWest Nile virus-infected Vero cells and influenza A H1N1-infected MDCK cells were treated with omeprazole and/ or ribavirin. Herpes simplex virus 1 (HSV-1)- or HSV-2-infected Vero or HaCat cells were treated with omeprazole and/ or acyclovir. Antiviral effects were determined by examination of cytopathogenic effects (CPE), immune staining, and virus yield assay. Cell viability was investigated by MTT assay.ResultsOmeprazole concentrations up to 80μg/mL did not affect the antiviral effects of ribavirin. In contrast, omeprazole increased the acyclovir-mediated effects on HSV-1- and HSV-2-induced CPE formation in a dose-dependent manner in Vero and HaCat cells. Addition of omeprazole 80μg/mL resulted in a 10.8-fold reduction of the acyclovir concentration that reduces CPE formation by 50% (IC50) in HSV-1-infected Vero cells and in a 47.7-fold acyclovir IC50 reduction in HSV-1-infected HaCat cells. In HSV-2-infected cells, omeprazole reduced the acyclovir IC50 by 7.3-fold (Vero cells) or by 12.9-fold (HaCat cells). Omeprazole also enhanced the acyclovir-mediated effects on viral antigen expression and virus replication in HSV-1- and HSV-2-infected cells. In HSV-1-infected HaCat cells, omeprazole 80μg/mL reduced the virus titre in the presence of acyclovir 1μg/mL by 1.6×105-fold. In HSV-2-infected HaCat cells omeprazole 80μg/mL reduced the virus titre in the presence of acyclovir 2μg/mL by 9.2×103-fold. The investigated drug concentrations did not affect cell viability, neither alone nor in combination.ConclusionsOmeprazole increases the anti-HSV activity of acyclovir. As clinically well-established and tolerated drug, it is a candidate drug for antiviral therapies in combination with acyclovir.

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Section: Effects Of Omeprazole In Combination With Acyclovir On Hsv-1mentioning

confidence: 99%