Intervertebral disc degeneration induced by intradiscal poly(methyl methacrylate) leakage after spine augmentation in an in vivo rabbit model

Mao, Haiqing; Geng, Dechun; Zhu, Xuesong; Ji, Shundong; Zhu, Mo; Gao, Maofeng; Zou, Jun; Yang, Huilin

doi:10.1016/j.actbio.2014.03.008

Cited by 11 publications

(7 citation statements)

References 35 publications

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“…They concluded that intradiscal injection of PMMA particles induced significant intervertebral disc degeneration in vivo. 16 In our study, the majority of patients had LGL (56.3%). The number of patients who had grade II (21.9%), grade III (15.6%) or grade IV (6.3%) leaks were relatively smaller.…”

Section: Discussionmentioning

confidence: 45%

Impact of the quantity of intradiscal cement leak on the progression of intervertebral disc degeneration

Jamjoom

Patel

Bommireddy

et al. 2017

annals

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Introduction We aim to assess the impact of the quantity of intradiscal cement leak during kyphoplasty on the rate of progression of degenerative changes in the affected disc. Methods Of 316 kyphoplasty procedures, we identified 32 episodes of intradiscal cement leak in 26 patients. The quantity of cement leaked was graded from I to IV. Disc degenerative changes were assessed at presentation and follow-up using radiographical scoring and magnetic resonance imaging (MRI) grading systems. Data for low-grade leaks (grade I) were compared with the medium- and high-grade leaks (grades II-IV) using a chi-squared test. Results Median follow-up radiographic and MRI assessments were made at 18 and 21 months, respectively. Medium- and high-grade leaks were associated with a significantly higher radiographic disc degeneration scores compared with low-grade leaks (P = 0.04295) but no difference was found in MRI disc degeneration grades and in adjacent vertebral fracture rates. Conclusions Our findings indicate that the quantity of cement leaking into the disc space significantly influences the rate of progression of disc degeneration.

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Section: Discussionmentioning

confidence: 45%

Impact of the quantity of intradiscal cement leak on the progression of intervertebral disc degeneration

Jamjoom

Patel

Bommireddy

et al. 2017

annals

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“…Surgical induced injury was the most common (168/259, 64.86%), followed by chemical injury (46/259, 17.76%) genetically modified (28/259, 10.81%) and spontaneous (28/259, 10.81%) models. For chemically induced degeneration, the two most common substances used were enzymes, via intradiscal, subchondral or systemic injection, 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 , 42 and poly (methyl methacrylate) (PMMA), via intradiscal PMMA particle injection or into the vertebral body parallel to the adjacent vertebral endplate 43 , 44 , 45 , 46 , 47 (Figure 4B ). Of the 28 (10.81%) papers that reported genetic modification to induce IVDD, 20 different genes were targeted for either gene knock‐out, 23 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 ,…”

Section: Resultsmentioning

confidence: 99%

Preclinical in vivo animal models of intervertebral disc degeneration. Part 1: A systematic review

et al. 2022

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Intervertebral disc degeneration (IVDD), a widely recognized cause of lower back pain, is the leading cause of disability worldwide. A myriad of preclinical in vivo animal models of IVDD have been described in the literature. There is a need for critical evaluation of these models to better inform researchers and clinicians to optimize study design and ultimately, enhance experimental outcomes. The purpose of this study was to conduct an extensive systematic literature review to report the variability of animal species, IVDD induction method, and experimental timepoints and endpoints used in in vivo IVDD preclinical research. A systematic literature review of peer‐reviewed manuscripts featured on PubMed and EMBASE databases was conducted in accordance with PRISMA guidelines. Studies were included if they reported an in vivo animal model of IVDD and included details of the species used, how disc degeneration was induced, and the experimental endpoints used for analysis. Two‐hundred and fifty‐nine (259) studies were reviewed. The most common species, IVDD induction method and experimental endpoint used was rodents(140/259, 54.05%), surgery (168/259, 64.86%) and histology (217/259, 83.78%), respectively. Experimental timepoint varied greatly between studies, ranging from 1 week (dog and rodent models), to >104 weeks in dog, horse, monkey, rabbit, and sheep models. The two most common timepoints used across all species were 4 weeks (49 manuscripts) and 12 weeks (44 manuscripts). A comprehensive discussion of the species, methods of IVDD induction and experimental endpoints is presented. There was great variability across all categories: animal species, method of IVDD induction, timepoints and experimental endpoints. While no animal model can replicate the human scenario, the most appropriate model should be selected in line with the study objectives to optimize experimental design, outcomes and improve comparisons between studies.

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“…In a study performed by Zhao et al, 24 weeks after injection of PMMA cement into the canine disc, X-ray and MRI examinations have revealed narrowing of disc space and reduced T2 signal intensity . In addition, histological examination showed decreased nucleus pulposus (the inner core of the intervertebral disc) and compressed extracellular matrix, as well as a significant increase in apoptotic cells. , Our previous work also found that PMMA particles can induce intervertebral disc degeneration in vivo . In addition, PMMA particles play a significant role in MMP-7 expression and apoptosis in nucleus pulposus cells, which may play a role in PMMA particle-induced intervertebral disc degeneration.…”

Section: Introductionmentioning

confidence: 82%

“…14,15 Our previous work also found that PMMA particles can induce intervertebral disc degeneration in vivo. 16 In addition, PMMA particles play a significant role in MMP-7 expression and apoptosis in nucleus pulposus cells, which may play a role in PMMA particle-induced intervertebral disc degeneration. Generally speaking, it seems that the effect of PMMA on the degeneration of intervertebral disc tissue, especially nucleus pulposus cells, is clear and definite.…”

Section: ■ Introductionmentioning

confidence: 99%

PMMA Bone Cement Acts on the Hippo/YAP Pathway To Regulate CTGF and Induce Intervertebral Disc Degeneration

Yang

Chen

et al. 2019

ACS Biomater. Sci. Eng.

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Kyphoplasty is a novel approach treating osteoporotic vertebral compression fractures and poly(methyl methacrylate) (PMMA) is the most common bone cement used for kyphoplasty. Cement leaked into an intervertebral disc (IVD) usually causes problems. Herein, we explored the effects of PMMA particles on the biological behavior of nucleus pulposus cells and proved the inhibiting effect of PMMA particles in a dose-dependent manner. Besides, PMMA particles led to cell cycle arrest and the abnormal expression of several apoptosis associated gene. Futhermore, we found PMMA particles upregulated the expression of p-Mst1/2, p-Lats1, and p-YAP, whereas they downregulated the expression of YAP and CTGF, suggesting the activing effect on the Hippo/YAP/CTGF signaling pathway. By overexpression of YAP, PMMA-induced nucleus pulposus cell degeneration was reversed. In vivo study also showed that recombinant CTGF protein could alleviate PMMA-induced IVD degeneration in rats. To sum up, our study revealed the role of PMMA particles in inducing IVD degeneration by downregulating CTGF through Hippo/YAP pathway.

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Intervertebral disc degeneration induced by intradiscal poly(methyl methacrylate) leakage after spine augmentation in an in vivo rabbit model

Cited by 11 publications

References 35 publications

Impact of the quantity of intradiscal cement leak on the progression of intervertebral disc degeneration

Impact of the quantity of intradiscal cement leak on the progression of intervertebral disc degeneration

Preclinical in vivo animal models of intervertebral disc degeneration. Part 1: A systematic review

PMMA Bone Cement Acts on the Hippo/YAP Pathway To Regulate CTGF and Induce Intervertebral Disc Degeneration

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