Intestinal epithelial tight junctions and permeability can be rescued through the regulation of endoplasmic reticulum stress by amniotic fluid stem cells during necrotizing enterocolitis

Li, Bo; Lee, Carol; Chuslip, Sinobol; Lee, Dorothy; Biouss, George; Wu, Richard; Koike, Yuhki; Ip, Wan; Gonska, Tanja; Pierro, Agostino

doi:10.1096/fj.202001426r

Cited by 20 publications

(14 citation statements)

References 59 publications

(123 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Antje et al also confirmed that the extent of liver steatosis in humans is related to intestinal permeability ( 39 ). This permeability of the intestine depends on tight junctions composed of specialized proteins, such as occludin, ZO-1, claudins, and junctional adhesion molecules ( 40 ). In this study, CLA decreased the mRNA and protein expressions of occludin of the proximal colon in WT mice, which may weaken intercellular adhesion and increase permeability.…”

Section: Discussionmentioning

confidence: 99%

Conjugated linoleic acid ameliorates hepatic steatosis by modulating intestinal permeability and gut microbiota in ob/ob mice

Gao

Zhang

et al. 2022

Food & Nutrition Research

View full text Add to dashboard Cite

Background: Conjugated linoleic acid (CLA) is an effective supplement for reducing fat mass, but its effect on hepatic steatosis remains controversial. Objective: This study aims to evaluate the effect of CLA on liver fat accumulation, inflammation, gut microbiome, and intestinal barrier integrity. Design: Wild-type (WT) mice and ob/ob (OB) mice were randomly divided into four groups according to the treatment with/without 1% CLA: WT, WT mice treated with CLA (WT-CLA), OB, and OB mice treated with CLA (OB-CLA). Lipid metabolism and hepatic fat accumulation were evaluated by changes in histological and biochemical parameters. Gene expressions related to liver inflammation and intestinal barrier integrity were examined. The effect of CLA on the gut microbiota population was investigated. Results: The body weight, fatty tissue mass, and serum lipid levels of the WT-CLA group and OB-CLA group were separately lower than those of the WT group and OB group, but the livers of the WT-CLA group had more fatty lipids, higher triglyceride properties, and saturated fatty acid (FA) composition than those of the WT group, which was contrary to the effect of CLA on OB mice. Real time quantitative PCR results showed that CLA increased hepatic inflammation and intestinal permeability in the WT mice, while it significantly decreased the mRNA expression of liver TNF-α, IFN-γ, and IL-1β and markedly ameliorated intestinal tight junction proteins in the OB mice. The gut microbiota testing indicated a higher abundance of beneficial bacteria (e.g., Lachnoclostridium, Roseburia, Dubosiella, Oscillibacter, and Anaerostipes) and a lower abundance of pro-inflammatory bacteria (e.g., Tyzzerella and Alistipes) in the OB-CLA group than those of the OB group. Correlation analysis suggested that gut microbiota correlated with liver inflammation, intestinal permeability, and hepatic FA composition. Conclusion: CLA potentially contributed to ameliorating hepatic steatosis in OB mice via modulating liver inflammation, intestinal permeability, and gut microbiota, which suggests CLA is more suitable for people with obesity or overweight.

show abstract

Section: Discussionmentioning

confidence: 99%

Conjugated linoleic acid ameliorates hepatic steatosis by modulating intestinal permeability and gut microbiota in ob/ob mice

Gao

Zhang

et al. 2022

Food & Nutrition Research

View full text Add to dashboard Cite

show abstract

“…137 Furthermore, isolated amniotic stem cells restored tight junction protein expression in mice. 138…”

Section: Amniotic Fluidmentioning

confidence: 99%

Intestinal Epithelial Barrier Function and Necrotizing Enterocolitis

Managlia¹,

Yan²,

Plaen³

2022

Newborn

View full text Add to dashboard Cite

Necrotizing enterocolitis (NEC) is a major cause of morbidity and mortality in premature infants. NEC is characterized by intestinal tissue inflammation and necrosis. The intestinal barrier is altered in NEC, which potentially contributes to its pathogenesis by promoting intestinal bacterial translocation and stimulating the inflammatory response. In premature infants, many components of the intestinal barrier are immature. This article reviews the different components of the intestinal barrier and how their immaturity contributes to intestinal barrier dysfunction and NEC.

show abstract

“…Both stem cells were associated with significantly lower NEC incidence and severity as compared to the breastfed control group ( 30 ). Another key study on intestinal organoids co-cultured with AFSCs as well as AFSC-administration in NEC-induced C57BL/6 mice (born full term, term gestation is 18.5 days) demonstrated that AFSCs prevented epithelial permeability and tight junction disruption through induction of a protective endoplasmatic reticulum stress response ( 31 ). Noteworthy, when full term C57BL/6 mice were treated prior to disease induction, AFSCs but not MSCs prevented injury.…”

Section: Amniotic Fluid-based Therapy In Neonates With Necrotizing En...mentioning

confidence: 99%

Amniotic Fluid: A Perspective on Promising Advances in the Prevention and Treatment of Necrotizing Enterocolitis

et al. 2022

View full text Add to dashboard Cite

Necrotizing enterocolitis (NEC) is a common and potentially fatal disease that typically affects preterm (PIs) and very low birth weight infants (VLBWIs). Although NEC has been extensively studied, the current therapeutic approaches are unsatisfactory. Due to the similarities in the composition between human amniotic fluid (AF) and human breast milk (BM), which plays a protective role in the development of NEC in PIs and VLBWIs, it has been postulated that AF has similar effects on the outcome of NEC and potential therapeutic implications. AF has been long used for its diagnostic purposes and is often discarded after birth as “biological waste”. However, researchers have started to elucidate its therapeutic potential. Experimental studies in animal models have shown that diseases of various organ systems can possibly benefit from AF-based therapy. Hence, we have identified three approaches which show promising results for future clinical application in the prevention and/or treatment of NEC: (1) administration of processed AF (PAF) isolated from donor mothers, (2) administration of AF stem cells (AFSCs), and (3) administration of simulated AF (SAF) formulated to mimic the composition of physiological AF. We have highlighted the most important aspects that should be taken into account to guide further research on the clinical application of AF-based therapy. We hope that this review can provide a framework to identify the challenges of AF-based therapy and help to design future studies to better evaluate AF-based approaches for the treatment and/or prevention of NEC in PIs and VLBWIs.

show abstract

Intestinal epithelial tight junctions and permeability can be rescued through the regulation of endoplasmic reticulum stress by amniotic fluid stem cells during necrotizing enterocolitis

Cited by 20 publications

References 59 publications

Conjugated linoleic acid ameliorates hepatic steatosis by modulating intestinal permeability and gut microbiota in ob/ob mice

Conjugated linoleic acid ameliorates hepatic steatosis by modulating intestinal permeability and gut microbiota in ob/ob mice

Intestinal Epithelial Barrier Function and Necrotizing Enterocolitis

Amniotic Fluid: A Perspective on Promising Advances in the Prevention and Treatment of Necrotizing Enterocolitis

Contact Info

Product

Resources

About