Intestinal expression of genes encoding inflammatory mediators and gelatinases during Arcobacter butzleri infection of gnotobiotic IL-10 deficient mice

Heimesaat, Markus M.; Alter, Thomas; Bereswill, Stefan; Gölz, Greta

doi:10.1556/1886.2016.00005

Cited by 9 publications

(12 citation statements)

References 36 publications

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“…While it may be physiologically important for the host to maintain a certain well-balanced level of local inflammation as a proper response to enteropathogens, avoidance of extra-intestinal and systemic inflammatory sequelae were pivotal for host health integrity. This further supports the dichotomic properties of cytokines depending on the respective (patho-)physiological context and cytokine milieu determining whether the same cytokine acts rather pro- or anti-inflammatory [44, 45]. Our findings here further reinforce former data providing evidence for anti-inflammatory properties of VSL#3.…”

Section: Discussionsupporting

confidence: 89%

Amelioration of intestinal and systemic sequelae of murine Campylobacter jejuni infection by probiotic VSL#3 treatment

et al. 2017

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BackgroundThe incidence of human Campylobacter jejuni infections is progressively increasing worldwide. Probiotic compounds might open up valuable tools to decrease pathogen burden and subsequent pro-inflammatory immune responses, but in vivo data are scarce.Methods and resultsSecondary abiotic mice generated by broad-spectrum antibiotic treatment were perorally challenged with the commercial probiotic compound VSL#3 consisting of Streptococcus thermophilus, Bifidobacterium breve, Bifidobacterium longum, Bifidobacterium infantis, Lactobacillus acidophilus, Lactobacillus plantarum, Lactobacillus paracasei, and Lactobacillus delbrueckii ssp. bulgaricus) either 5 days before (i.e. prophylactic regimen) or after (i.e. therapeutic regimen) peroral C. jejuni strain 81–176 infection, and analyzed 3 weeks following the initial bacterial re-association. Upon challenge, mice were colonized with the probiotic bacteria and/or C. jejuni at comparable intestinal loads, but co-colonization did not result in reduction of the pathogen burden. Remarkably, prophylactic as well as therapeutic VSL#3 treatment of C. jejuni infected mice ameliorated intestinal apoptosis and pro-inflammatory immune responses as indicated by lower numbers of innate and adaptive immune cell populations in the murine colon upon probiotic prophylaxis or treatment and reduced colonic concentrations of pro-inflammatory mediators including IL-6 and MCP-1. Importantly, concentrations of anti-inflammatory mediators such as IL-10 were significantly elevated in the colon of probiotics treated mice as compared to untreated controls. Strikingly, prophylactic VSL#3 treatment attenuated C. jejuni induced systemic pro-inflammatory responses as indicated by less TNF and IL-12p70 secretion in the spleen of VSL#3 pre-treated as compared to non-treated mice.ConclusionAdministration of probiotic formulations such as VSL#3 might open up valuable strategies for prophylaxis and/or treatment of C. jejuni induced intestinal and systemic sequelae in vivo by the suppression of pro-inflammatory and induction of anti-inflammatory responses.

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Section: Discussionsupporting

confidence: 89%

Amelioration of intestinal and systemic sequelae of murine Campylobacter jejuni infection by probiotic VSL#3 treatment

et al. 2017

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“…A. butzleri infection in gnotobiotic IL-10 -/-mice showed up-regulation of mRNA levels of proinflammatory cytokines such as TNF and IL-1b, and MMP-2 and MMP-9 in the intestine (Heimesaat et al 2016). …”

Section: Disease In Animals Caused By Arcobactersmentioning

confidence: 98%

“…However, infected mice did not show any overt clinical signs, the bacteria not only induced intestinal immune responses but also systemic immune responses in extra-intestinal places depending on the strain and time course of infection Heimesaat et al 2015a). These immune responses were TLR-4 dependent recognized by LOS and LPS derived from the cell walls of Gramnegative bacteria in gnotobiotic IL-10 -/-mice lacking TLR-4 with less pronounced intestinal and systemic immune responses Heimesaat et al 2015b;Heimesaat et al 2016). The mucin-2 is a pivotal constituent of the intestinal mucosa providing first line defense against intestinal pathogens (Strugala et al 2003) and was downregulated in the colon, but not in the ileum of A. butzleri infected mice (G€ olz et al 2016b;Heimesaat et al 2016).…”

Section: Disease In Animals Caused By Arcobactersmentioning

confidence: 99%

“…These immune responses were TLR-4 dependent recognized by LOS and LPS derived from the cell walls of Gramnegative bacteria in gnotobiotic IL-10 -/-mice lacking TLR-4 with less pronounced intestinal and systemic immune responses Heimesaat et al 2015b;Heimesaat et al 2016). The mucin-2 is a pivotal constituent of the intestinal mucosa providing first line defense against intestinal pathogens (Strugala et al 2003) and was downregulated in the colon, but not in the ileum of A. butzleri infected mice (G€ olz et al 2016b;Heimesaat et al 2016). A. butzleri induces antimicrobial host immunity and cytokines expression such as IL-17A, IL-22, IFN-g and their master regulator IL-23 in intestine that are secreted by T helper (Th) 17 cells.…”

Section: Disease In Animals Caused By Arcobactersmentioning

confidence: 99%

“…During A. butzleri infection in murine, IL-22 and IL-18 in intestine were upregulated in a strain-dependent manner indicating that the IL-22/IL-18 axis might be responsible for regulating the immune responses during arcobacteriosis (G€ olz et al 2016aHeimesaat et al 2016). A. butzleri infection in gnotobiotic IL-10 -/-mice showed up-regulation of mRNA levels of proinflammatory cytokines such as TNF and IL-1b, and MMP-2 and MMP-9 in the intestine (Heimesaat et al 2016).…”

Section: Disease In Animals Caused By Arcobactersmentioning

confidence: 99%

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Arcobacter: an emerging food-borne zoonotic pathogen, its public health concerns and advances in diagnosis and control – a comprehensive review

Ramees

Dhama

Karthik

et al. 2017

Veterinary Quarterly

138

130

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Arcobacter has emerged as an important food-borne zoonotic pathogen, causing sometimes serious infections in humans and animals. Newer species of Arcobacter are being incessantly emerging (presently 25 species have been identified) with novel information on the evolutionary mechanisms and genetic diversity among different Arcobacter species. These have been reported from chickens, domestic animals (cattle, pigs, sheep, horses, dogs), reptiles (lizards, snakes and chelonians), meat (poultry, pork, goat, lamb, beef, rabbit), vegetables and from humans in different countries. Arcobacters are implicated as causative agents of diarrhea, mastitis and abortion in animals, while causing bacteremia, endocarditis, peritonitis, gastroenteritis and diarrhea in humans. Three species including A. butzleri, A. cryaerophilus and A. skirrowii are predominantly associated with clinical conditions. Arcobacters are primarily transmitted through contaminated food and water sources. Identification of Arcobacter by biochemical tests is difficult and isolation remains the gold standard method. Current diagnostic advances have provided various molecular methods for efficient detection and differentiation of the Arcobacters at genus and species level. To overcome the emerging antibiotic resistance problem there is an essential need to explore the potential of novel and alternative therapies. Strengthening of the diagnostic aspects is also suggested as in most cases Arcobacters goes unnoticed and hence the exact epidemiological status remains uncertain. This review updates the current knowledge and many aspects of this important food-borne pathogen, namely etiology, evolution and emergence, genetic diversity, epidemiology, the disease in animals and humans, public health concerns, and advances in its diagnosis, prevention and control.

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Arcobacter butzleri: Up‐to‐date taxonomy, ecology, and pathogenicity of an emerging pathogen

Chieffi

Fanelli

Fusco

2020

Comp Rev Food Sci Food Safe

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Arcobacter butzleri, recently emended to the Aliarcobacter butzleri comb. nov., is an emerging pathogen causing enteritis, severe diarrhea, septicaemia, and bacteraemia in humans and enteritis, stillbirth, and abortion in animals. Since its recognition as emerging pathogen on 2002, advancements have been made in elucidating its pathogenicity and epidemiology, also thanks to advent of genomics, which, moreover, contributed in emending its taxonomy. In this review, we provide an overview of the up‐to‐date taxonomy, ecology, and pathogenicity of this emerging pathogen. Moreover, the implication of A. butzleri in the safety of foods is pinpointed, and culture‐dependent and independent detection, identification, and typing methods as well as strategies to control and prevent the survival and growth of this pathogen are provided.

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Intestinal expression of genes encoding inflammatory mediators and gelatinases during Arcobacter butzleri infection of gnotobiotic IL-10 deficient mice

Cited by 9 publications

References 36 publications

Amelioration of intestinal and systemic sequelae of murine Campylobacter jejuni infection by probiotic VSL#3 treatment

Amelioration of intestinal and systemic sequelae of murine Campylobacter jejuni infection by probiotic VSL#3 treatment

Arcobacter: an emerging food-borne zoonotic pathogen, its public health concerns and advances in diagnosis and control – a comprehensive review

Arcobacter butzleri: Up‐to‐date taxonomy, ecology, and pathogenicity of an emerging pathogen

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