Intestinal Fatty Acid Binding Protein (I-FABP) for the Detection of Strangulated Mechanical Small Bowel Obstruction

Cronk, Daniel R.; Houseworth, Troy P.; Cuadrado, Daniel G.; Herbert, Garth S.; McNutt, Patrick; Azarow, Kenneth S.

doi:10.1016/j.cursur.2006.05.006

Cited by 95 publications

(93 citation statements)

References 15 publications

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“…The potential clinical values of HDC and CP might be best discussed in the context of the established serum biomarkers (D-lactate, α-GST, I-FABP and DAO) and urine biomarkers (I-FABP and TXB2). Although theses established biomarkers are not recommended for detection of IMI in patients with AIO because of the lack of sensitivity and specificity, they are still useful indicators for IMI in many current studies on IMI (19)(20)(21)(22)(23)(24)(25)29,30). The AUCs reached 0.937 and 0.770 for serum HDC and CP and 0.916 and 0.708 for urine HDC and CP, respectively, using ROC curve analysis.…”

Section: O V E M B E R -D E C E M B E R 2 0 1 1 H I S T I D I N E D Ementioning

confidence: 99%

“…However, the traditional diagnosis methods for diagnosing IMI in patients with the above-mentioned diseases, especially AIO, on the basis of fever, chills, peritonitis, tachycardia, leukocytosis and acidosis, are notoriously unreliable, even in the hands of experienced surgeons (17,18). Several serum biomarkers including D-lactate (19)(20)(21), α-glutathione S-transferase (α-GST) (22,23), intestinal fatty acid binding proteins (I-FABP) (24,25), D-dimer (26,27), cobalt-albumin binding assay (28), diamine oxidase (DAO) (29) and urine biomarkers, including I-FABP (24) and thromboxane B2 (TXB2) (30) for IMI, were investigated; however, none of these biomarkers was proven clinically useful because of poor sensitivity and specificity. Therefore, identification of novel and effective biomarkers for the prognosis of IMI and the response to treatment in patients with AIO is urgently required.…”

Section: Introductionmentioning

confidence: 99%

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Histidine Decarboxylase Is Identified as a Potential Biomarker of Intestinal Mucosal Injury in Patients with Acute Intestinal Obstruction

Yang

Zhāng

et al. 2011

Mol Med

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Various biomarkers currently used for the diagnosis of intestinal mucosal injury (IMI) in patients with acute intestinal obstruction have low sensitivity and specificity. In the present study, IMI, as indicated by the impaired expression of tight junction proteins, including zonula occludens-1, occludin and claudin-1, and inflammation were determined in colonic tissues of patients with 45 strangulated intestinal obstruction (STR-IO) and the adjacent "normal" colonic tissues of 35 patients with colon cancers by quantitative real-time polymerase chain reaction (QRT-PCR), Western blotting, immunohistochemistry and histological examination, respectively. Then, two-dimensional fluorescent difference gel electrophoresis coupled with linear trap quadrupole mass spectrometry was used to screen for potential biomarkers of IMI in the serum samples of 10 STR-IO, 10 simple intestinal obstruction (SIM-IO) and 10 normal healthy controls. A total of 35 protein spots were differentially expressed among the serum samples, and six of the proteins were identified as potential biomarkers. Among the six proteins, histidine decarboxylase (HDC) and ceruloplasmin (CP) were elevated significantly in patients with STR-IO, compared with patients with SIM-IO and healthy controls. Thus, HDC and CP were further validated by QRT-PCR, Western blotting, immunohistochemistry and enzyme-linked immunosorbent assay, respectively, in colonic tissues, serum and urine samples. Finally, the receiver operating characteristic curves were used to show the area under the curves of HDC, CP and several established biomarkers, followed by the determination of the appropriate cutoff values and their sensitivities and specificities. It was shown that for serum and urine, HDC levels achieved sensitivities and specificities compatible to or even greater than those of established biomarkers for the diagnosis of IMI in patients with acute intestinal obstruction, although further validation in a larger cohort is required.

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Section: O V E M B E R -D E C E M B E R 2 0 1 1 H I S T I D I N E D Ementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Histidine Decarboxylase Is Identified as a Potential Biomarker of Intestinal Mucosal Injury in Patients with Acute Intestinal Obstruction

Yang

Zhāng

et al. 2011

Mol Med

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“…De Laurier et al 9 reported that plasma lactate levels increased after 8 hours of experimental bowel strangulation in dogs, and several studies and experimental models have shown serum lactate levels to be useful in the accurate preoperative diagnosis of bowel strangulation. [9][10][11][12][13][14][15] In our studies, the time lag between strangulation and increase of lactate is 8 hours.…”

Section: Discussionmentioning

confidence: 99%

Lactate Levels in Bowel Strangulation With Experimental Animal Model

Tanaka

Hashimoto

Ohki

2015

International Surgery

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Bowel strangulation is a common disease and often requires an urgent operation. Our previous report showed that lactate concentration in the blood is a good predictive marker for emergency. However, the alteration in lactate levels during the course of bowel strangulation remains unclear. We have investigated the progressive increase of lactate after induction of bowel strangulation with animal experiments. Thirty-six mice were randomly divided into 6 groups: 0, 8, 16, 24, 48, and 72 hours after operation. Under anesthesia, laparotomy was performed and a 5-cm segment of terminal ileum with the mesenteric artery and veins was ligated to create a strangulation obstruction. After operation, the arterial blood was collected and lactate concentration and pH were measured using a blood gas analyzer. Lactate concentration in the 8-hour group was markedly increased, while pH decreased significantly (P , 0.05). Lactate concentrations were kept at a high level from 8 to 72 hours after operation. The time lag between strangulation and an increase of lactate is 8 hours. Our study is the first report of a bowel strangulation mouse model. Therefore, it would be possible to find a more specific marker of bowel strangulation using our experimental model.

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“…Отмечена достоверная разница в уров-не i-fabp у больных с неязвенным колитом в зави-симости от локализации и активности заболевания, у больных с левосторонним колитом средние значе-ния составляли 61,8±8,5 пг/мл, а у больных с панко-литом достоверно повышалась до 260,5±606 пг/мл [11]. Повышение уровня i-fabp наблюдали и у боль-ных с странгуляционной непроходимостью тонкой кишки [12], и, по мнению авторов, с чувствительно-стью 100% и специфичностью 83% его повышение может служить признаком некроза тонкой кишки.…”

Section: ключевые слова: I-fabp повреждения кишечникаunclassified

The fatty acids binding protein (I-FABP) — the diagnostic marker of damage of the intestine

Li¹,

Nosková²,

Варванина³

et al. 2015

Lab. sluzh.

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Intestinal Fatty Acid Binding Protein (I-FABP) for the Detection of Strangulated Mechanical Small Bowel Obstruction

Cited by 95 publications

References 15 publications

Histidine Decarboxylase Is Identified as a Potential Biomarker of Intestinal Mucosal Injury in Patients with Acute Intestinal Obstruction

Histidine Decarboxylase Is Identified as a Potential Biomarker of Intestinal Mucosal Injury in Patients with Acute Intestinal Obstruction

Lactate Levels in Bowel Strangulation With Experimental Animal Model

The fatty acids binding protein (I-FABP) — the diagnostic marker of damage of the intestine

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