Intestinal flora in the constipation patients before versus after lactulose intervention

Ma, Jianxia; Ma, Houlian; Zheng, Songbai; Yu, Xiaofeng; Wang, Ke; Wang, Jun; Pan, Yue; Yao, Jianfeng

doi:10.1097/md.0000000000034703

Cited by 5 publications

(2 citation statements)

References 38 publications

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“…Saccharoferments in the rumen microbiota can promote the production of low-density lipoprotein cholesterol (LDL-C), reduce the relative abundance of Saccharoferments, and lower blood lipids ( 49 ). Mogibacterium is usually abundant in the colon mucosa of human colorectal cancer patients, so an increase in its relative abundance may damage the health of goat rumen epithelium ( 50 , 51 ). Analysis of fecal microbiota showed that compared to the CON group, the relative abundance of Papilibacter, Pseudoflavonifractor, Butyricicoccus, Anaerovorax, and Metanocorpusculum in the ISL group was significantly upregulated; Roseburia, Coprococcus, Clostridium XVIII, Butyrivibrio, Parasutterella, Macellibacteroides, and Porphyromonas were significantly downregulated.…”

Section: Discussesmentioning

confidence: 99%

Multi-omics analysis on the mechanism of the effect of Isatis leaf on the growth performance of fattening sheep

Cao,

Yi,

Zhou

et al. 2024

Front. Vet. Sci.

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IntroductionThis study evaluated the effects of Isatis Leaf (ISL) on the growth performance, gastrointestinal tissue morphology, rumen and intestinal microbiota, rumen, serum and urine metabolites, and rumen epithelial tissue transcriptome of fattening sheep.MethodsTwelve 3.5-month-old healthy fattening sheep were randomly divided into two groups, each with 6 replicates, and fed with basal diet (CON) and basal diet supplemented with 80 g/kg ISL for 2.5 months. Gastrointestinal tract was collected for histological analysis, rumen fluid and feces were subjected to metagenomic analysis, rumen fluid, serum, and urine for metabolomics analysis, and rumen epithelial tissue for transcriptomics analysis.ResultsThe results showed that in the ISL group, the average daily gain and average daily feed intake of fattening sheep were significantly lower than those of the CON group (P < 0.05), and the rumen ammonia nitrogen level was significantly higher than that of the CON group (P < 0.01). The thickness of the reticulum and abomasum muscle layer was significantly increased (P < 0.05). At the genus level, the addition of ISL modified the composition of rumen and fecal microorganisms, and the relative abundance of Methanobrevibacter and Centipeda was significantly upregulated in rumen microorganisms, The relative abundance of Butyrivibrio, Saccharofermentans, Mogibacterium, and Pirellula was significantly downregulated (P < 0.05). In fecal microorganisms, the relative abundance of Papillibacter, Pseudoflavonifractor, Butyricicoccus, Anaerovorax, and Methanocorpusculum was significantly upregulated, while the relative abundance of Roseburia, Coprococcus, Clostridium XVIII, Butyrivibrio, Parasutterella, Macellibacteroides, and Porphyromonas was significantly downregulated (P < 0.05). There were 164, 107, and 77 different metabolites in the rumen, serum, and urine between the ISL and CON groups (P < 0.05). The differential metabolic pathways mainly included thiamine metabolism, niacin and nicotinamide metabolism, vitamin B6 metabolism, taurine and taurine metabolism, beta-Alanine metabolism and riboflavin metabolism. These metabolic pathways were mainly involved in the regulation of energy metabolism and immune function in fattening sheep. Transcriptome sequencing showed that differentially expressed genes were mainly enriched in cellular physiological processes, development, and immune regulation.ConclusionIn summary, the addition of ISL to the diet had the effect of increasing rumen ammonia nitrogen levels, regulating gastrointestinal microbiota, promoting body fat metabolism, and enhancing immunity in fattening sheep.

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Section: Discussesmentioning

confidence: 99%

Multi-omics analysis on the mechanism of the effect of Isatis leaf on the growth performance of fattening sheep

Cao,

Yi,

Zhou

et al. 2024

Front. Vet. Sci.

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“…Lactulose dose-dependently increases the frequency of spontaneous defecation and has a good safety profile[ 22 ]. Lactulose exerts its therapeutic effect by acting as an osmotic laxative; increasing the abundance of beneficial intestinal microbes, such as Bifidobacterium ; and improving the balance of intestinal microbiota and metabolites, thus inhibiting intestinal inflammatory response and promoting intestinal movement[ 37 , 38 ].…”

Section: Gut Microbiota Modulation In Fcmentioning

confidence: 99%

Microbiota treatment of functional constipation: Current status and future prospects

Li,

Zhang,

Wang

2024

World J Hepatol

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Functional constipation (FC) is a common disorder that is characterized by difficult stool passage, infrequent bowel movement, or both. FC is highly prevalent, recurs often, accompanies severe diseases, and affects quality of life; therefore, safe and effective therapy with long-term benefits is urgently needed. Microbiota treatment has potential value for FC treatment. Microbiota treatments include modulators such as probiotics, prebiotics, synbiotics, postbiotics, and fecal microbiota transplantation (FMT). Some probiotics and prebiotics have been adopted, and the efficacy of other microbiota modulators is being explored. FMT is considered an emerging field because of its curative effects; nevertheless, substantial work must be performed before clinical implementation.

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Review of history and mechanisms of action of lactulose (4-O-β-D-Galactopyranosyl-β-D-fructofuranose): present and future applications in food

Vičič,

Pandel Mikuš,

Ferjančič

2024

J Food Sci Technol

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Lactulose is a synthetic disaccharide composed of galactose and fructose. Literature review of history, legal status and possible food applications of lactulose in functional foods, such as confectionery and beverages. In the colon, lactulose is fermented by the microbiota and acts as a selective modulator of bacterial growth, promoting the growth of Lactobacilli and Bifidobacteria. It generates organic acids, such as short-chain fatty acids and lactic acid, which lower the pH of the colon and act as an osmotic laxative. Lactulose was first used in 1957 as an ingredient in an infant formula. Later it was registered as a prescription drug and banned for food use in many countries. In 2012, lactulose received an EU (European union) health claim “contributes to acceleration of intestinal transit”. It can be used in food and food supplements across all age groups, from infants to the elderly. Lactulose has favourable technological properties, such as sweetness of 48–62% sucrose without an aftertaste, high solubility, low cariogenic potential and stability. Lactulose gummy candy, without added sweeteners, has an overall likability comparable to classic sucrose/glucose-based candy. With more than 60 years of safe use in infant, child, adult and elderly population, lactulose is an ideal ingredient for prebiotic functional food. Its technological properties allow for development of functional candy and beverages almost indistinguishable from those made from sucrose.

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Intestinal flora in the constipation patients before versus after lactulose intervention

Cited by 5 publications

References 38 publications

Multi-omics analysis on the mechanism of the effect of Isatis leaf on the growth performance of fattening sheep

Multi-omics analysis on the mechanism of the effect of Isatis leaf on the growth performance of fattening sheep

Microbiota treatment of functional constipation: Current status and future prospects

Review of history and mechanisms of action of lactulose (4-O-β-D-Galactopyranosyl-β-D-fructofuranose): present and future applications in food

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