Intestinal healing in rats submitted to ethanol ingestion

Pereira, Rodrigo Severo de Camargo; Hasimoto, Cláudia Nishida; Pelafsky, Leonardo; Llanos, Juan Carlos; Catâneo, Daniele Cristina; Spadella, César Tadeu; Minossi, José Guilherme

doi:10.1590/s0102-86502012000300006

Cited by 6 publications

(8 citation statements)

References 47 publications

(48 reference statements)

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“…Although the high incidence of chronic lung disease in alcoholics has been accepted and documented, little is known about the effects of alcohol on the lungs. The interrelation between smoking and body weight 1 and alcohol consumption and body weight 2,3 has been consolidated by several studies; however, none of these have considered the combination of both drugs.…”

Section: Introductionmentioning

confidence: 99%

Lung morphology and growth of rats exposed to tobacco smoke and alcohol

Magnani¹,

Catâneo

Capelozzi

et al. 2012

Acta Cir. Bras.

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PURPOSE:Investigate the morphological effects of chronic exposure to tobacco smoke inhalation and alcohol consumption on the lungs and on the growth of rats. METHODS: Sixty male Wistar rats were divided into four groups: control, tobacco, alcohol, tobacco + alcohol, for a period of study 260 days. Morphological analysis was conducted by optical and electron microscopy. Rat growth was investigated by measuring the snout-anus length, body mass index and body weight. RESULTS:The three groups exposed to the drugs presented lower growth and lower weight than the control group. The percentages of alveolitis, bronchiolitis and the mean alveolar diameter were greater, particularly in the groups exposed to tobacco smoke, but were not significantly different from the control group. Electron microscopy revealed more intense apoptotic and degenerative lesions in the smoking group, while degenerative lesions in the lamellar bodies were more intense with the association of both drugs. CONCLUSIONS: This experimental model showed morphological alterations observed by electron microscopy, principally due to tobacco smoke exposure. Alcohol and tobacco hindered the growth of rats, such that tobacco showed a greater effect on body length and alcohol on body weight. Key words: Alcoholism. Smoking. Lung Diseases. Rats. RESUMO OBJETIVO:Investigar os efeitos morfológicos da exposição crônica à inalação de fumaça do tabaco e o do consumo de álcool nos pulmões e no crescimento de ratos. MÉTODOS: Sessenta ratos Wistar machos foram distribuídos em quatro grupos: controle, tabaco, álcool e tabaco + álcool, e acompanhados por um período de 260 dias. No final do periodo foi realizada análise morfológica dos pulmões por microscopia óptica e eletrônica. O crescimento dos ratos foi investigado através da medição do comprimento focinho-ânus, peso corporal e índice de massa corporal. RESULTADOS: Os três grupos expostos às drogas apresentaram peso e comprimento significativamente menores que os do grupo controle. As percentagens de bronquiolite e alveolite, e o diâmetro alveolar médio foram maiores nos grupos expostos à fumaça do tabaco, mas sem significancia estatística quando comparadas ao grupo controle. A microscopia eletrônica revelou apoptose mais intensa e lesões degenerativas no grupo de fumantes, enquanto lesões degenerativas nos corpos lamelares foram mais intensas com a associação de ambas as drogas. CONCLUSÕES: Este modelo experimental mostrou alterações morfológicas observadas por microscopia eletrônica, principalmente devido à exposição ao tabaco. Tanto o alcool como o tabaco prejudicaram o crescimento dos animais, o tabaco mostrando um efeito maior sobre o comprimento e o álcool sobre o peso corporal. Descritores: Alcoolismo.Tabagismo. Pneumopatias. Ratos.Magnani KL et al.

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Section: Introductionmentioning

confidence: 99%

Lung morphology and growth of rats exposed to tobacco smoke and alcohol

Magnani¹,

Catâneo

Capelozzi

et al. 2012

Acta Cir. Bras.

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“…Both alcohol and sepsis are known to lead to weight loss after surgery, and their combination diminished the post-operative body mass in this study, and even at the 7 POD that weight wasn’t recovered [13,14]. Sepsis leads to a consumptive syndrome due to the inflammation and alcohol intake is responsible for malnutrition because of intestinal malabsorption and is also responsible for body fat reduction [13,14].…”

Section: Discussionmentioning

confidence: 88%

Alcohol acute intoxication before sepsis impairs the wound healing of intestinal anastomosis: rat model of the abdominal trauma patient

et al. 2012

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IntroductionMost trauma patients are drunk at the time of injury. Up to 2% of traumatized patients develop sepsis, which considerably increases their mortality. Inadequate wound healing of the colonic repair can lead to postoperative complications such as leakage and sepsis.ObjectiveTo assess the effects of acute alcohol intoxication on colonic anastomosis wound healing in septic rats.MethodsThirty six Wistar rats were allocated into two groups: S (induction of sepsis) and AS (alcohol intake before sepsis induction). A colonic anastomosis was performed in all groups. After 1, 3 or 7 days the animals were killed. Weight variations, mortality rate, histopathology and tensile breaking strength of the colonic anastomosis were evaluated.ResultsThere was an overall mortality of 4 animals (11.1%), three in the group AS (16.6%) and one in the S group (5.5%). Weight loss occurred in all groups. The colon anastomosis of the AS group didn’t gain strength from the first to the seventh postoperative day. On the histopathological analysis there were no differences in the deposition of collagen or fibroblasts between the groups AS and S.ConclusionAlcohol intake increased the mortality rate three times in septic animals. Acute alcohol intoxication delays the acquisition of tensile strength of colonic anastomosis in septic rats. Therefore, acute alcohol intoxication before sepsis leads to worse prognosis in animal models of the abdominal trauma patients.

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“…Pereira et al 9 has shown that a model of chronic alcohol abuse in rats is associated with lower weight gain, impaired intestinal healing (as demonstrated by significantly lower bowel anastomosis breaking strength), and higher postoperative complication rates.…”

Section: Discussionmentioning

confidence: 99%

“…We hypothesized that acute alcohol exposure shortly before surgery would be a risk factor for impaired wound healing of the colonic anastomosis, based on results from other experimental studies [9][10][11][12] . However, in those studies, alcohol exposure followed different patterns.…”

Section: Introductionmentioning

confidence: 99%

Does acute alcohol intoxication interfere with colonic anastomosis wound healing?: a rat model of nondestructive colon trauma

Morais¹,

Farias²,

Ribeiro³

et al. 2013

Acta Cir. Bras.

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PURPOSE:To evaluate the effects of acute alcohol intoxication on healing of colonic anastomosis. METHODS: Thirty-six rats were allocated into two groups. Animals in the alcohol (A) were given 2 mL of ethanol diluted in 0.9% saline solution to a concentration of 40% by gavage immediately before anesthesia, whereas control (C) animals received 2 mL of 0.9% saline solution via the same route. A colonic anastomosis was then performed in all animals. On postoperative days 1, 3, and 7, anastomotic breaking strength was assessed and histopathological examination was performed. Change in body weight and mortality were also evaluated. RESULTS:The median of anastomotic tensile strength on the postoperative day 1 was 0.09 Newtons for group A and 0.13 for group C. (p>0.05). The median of anastomotic tensile strength on the postoperative day 3 was 0.13 Newtons for group A and 0.17 for group C. (p>0.05). The median of anastomotic tensile strength on the postoperative day 7 was 0.30 Newtons for group A and 0.35 for group C. (p>0.05). There was no significant difference between the groups A and C, in the first, third or seventh POD (p>0.05), in any of the analyzed parameters. There were no statistical differences between groups in the weight. Three animals died, all from the group A. CONCLUSION:Acute alcohol intoxication did not interfere with wound healing of colonic anastomoses, although it caused early postoperative mortality.

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Intestinal healing in rats submitted to ethanol ingestion

Cited by 6 publications

References 47 publications

Lung morphology and growth of rats exposed to tobacco smoke and alcohol

Lung morphology and growth of rats exposed to tobacco smoke and alcohol

Alcohol acute intoxication before sepsis impairs the wound healing of intestinal anastomosis: rat model of the abdominal trauma patient

Does acute alcohol intoxication interfere with colonic anastomosis wound healing?: a rat model of nondestructive colon trauma

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