Intestinal inflammatory profile shows increase in a diversity of biomarkers in irritable bowel syndrome

Berg, Leif Kyrre; Goll, Rasmus; Fagerli, Erik; Ludviksen, Judith Krey; Fure, Hilde; Moen, Odd Sverre; Sørbye, Sveinung Wergeland; Mollnes, Tom Eirik; Florholmen, Jon

doi:10.1080/00365521.2020.1754455

Cited by 17 publications

(7 citation statements)

References 44 publications

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“…Our results demonstrated that there was a statistically significant reduction in serum IL-8 in the ethosuximide group compared with the mebeverine group. The increasing levels of IL-8 in IBS patients in our study are in agreement with several studies 6,[17][18][19][20] .…”

Section: Discussionsupporting

confidence: 93%

Open‐label pilot study of ethosuximide as adjunctive therapy for relieving abdominal pain related to Irritable Bowel Syndrome

El-Haggar

Hegazy

Bahaa

2021

Clinical Pharmacy Therapeu

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Irritable bowel syndrome (IBS) is a chronic gastrointestinal condition associated with abdominal discomfort, combination of abdominal pain associated with a change in the frequency or form of stool, bloating and alteration in bowel habits without an identifiable organic reason 1 . This functional disorder is extremely common, with prevalence estimates ranging from 10% to 15% in Europe and the United States. IBS has a significant impact on the quality of life of patients and results in substantial healthcare costs 2 . The pathophysiology

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Section: Discussionsupporting

confidence: 93%

Open‐label pilot study of ethosuximide as adjunctive therapy for relieving abdominal pain related to Irritable Bowel Syndrome

El-Haggar

Hegazy

Bahaa

2021

Clinical Pharmacy Therapeu

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“…Various results have been reported for cytokines at the mucosal level in IBS patients: increased expression of IL-1b [ 16 ], TNF-a [ 17 ], decreased levels of IL-10 [ 4 , 18 ] and IL-8 [ 19 ]. These changes can lead to the activation of the immune response, cytokines disbalances, and low-grade mucosal inflammation.…”

Section: Introductionmentioning

confidence: 99%

Disruption of the pro-inflammatory, anti-inflammatory cytokines and tight junction proteins expression, associated with changes of the composition of the gut microbiota in patients with irritable bowel syndrome

et al. 2021

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Background Irritable bowel syndrome (IBS) is a pathologic condition characterized by changes in gut microbiome composition, low-grade inflammation, and disruption of intestinal wall permeability. The interaction between the gut microbiome and the disease manifestation remains unclear. The changing of tight junction proteins and cytokines expression throughout the gastrointestinal tract in IBS patients has not been studied yet. Aim of the study To assess the changes of gut microbiome composition, tight junction proteins, and cytokines expression of intestinal mucosa from the duodenum to the distal part of the colon in IBS patients and healthy volunteers. Methods In 31 IBS patients (16 patients with IBS-D; 15 patients with IBS-C) and 10 healthy volunteers the expression of CLD-2, CLD-3, CLD-5, IL-2, IL-10, and TNF-α in mucosal biopsy specimens was determined by morphological and immune-histochemical methods. The qualitative and quantitative composition of the intestinal microbiota was assessed based on 16S rRNA gene sequencing in both groups of patients. Results The expression of IL-2 and TNF-α was significantly increased in IBS patients compared with the controls (p<0.001), with a gradual increase from the duodenum to the sigmoid colon. The expression of IL-10, CLD-3, and CLD-5 in mucosal biopsy specimens of these patients was lower than in the control group (p<0.001). Increased ratios of Bacteroidetes and decreased ratios of Firmicutes were noted in IBS patients compared to healthy volunteers (p<0.05). Conclusion IBS patients have impaired gut permeability and persisting low-grade inflammation throughout the gastrointestinal tract. Changes in the gut microbiota may support or exacerbate these changes.

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“…Furthermore, our RNA-seq study revealed significant increases in chemokines and chemokine receptors including CCL2 , CCL6 , CCL11 , CCL20 , CCL28 , CCR1 , CCR5 and CXCL10 . Increased chemokines such as CCL2 and CCL20 at mucosal has recently been reported in IBS patients ( Hayatbakhsh et al, 2019 ; Berg et al, 2020 ; Camilleri et al, 2014 ). The cytokine and chemokine expression has distinguished profiles between patients with IBS and IBD, suggesting that inflammatory mechanisms of the diseases are part of different spectrums ( Moraes et al, 2020 ) yet may overlap in certain circumstances such as increased immune response and increased gut permeability ( Spiller & Major, 2016 ).…”

Section: Discussionmentioning

confidence: 93%

Chronic psychological stress alters gene expression in rat colon epithelial cells promoting chromatin remodeling, barrier dysfunction and inflammation

Wiley

Higgins

Hong

2022

PeerJ

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Chronic stress is commonly associated with enhanced abdominal pain (visceral hypersensitivity), but the cellular mechanisms underlying how chronic stress induces visceral hypersensitivity are poorly understood. In this study, we examined changes in gene expression in colon epithelial cells from a rat model using RNA-sequencing to examine stress-induced changes to the transcriptome. Following chronic stress, the most significantly up-regulated genes included Atg16l1, Coq10b, Dcaf13, Nat2, Ptbp2, Rras2, Spink4 and down-regulated genes including Abat, Cited2, Cnnm2, Dab2ip, Plekhm1, Scd2, and Tab2. The primary altered biological processes revealed by network enrichment analysis were inflammation/immune response, tissue morphogenesis and development, and nucleosome/chromatin assembly. The most significantly down-regulated process was the digestive system development/function, whereas the most significantly up-regulated processes were inflammatory response, organismal injury, and chromatin remodeling mediated by H3K9 methylation. Furthermore, a subpopulation of stressed rats demonstrated very significantly altered gene expression and transcript isoforms, enriched for the differential expression of genes involved in the inflammatory response, including upregulation of cytokine and chemokine receptor gene expression coupled with downregulation of epithelial adherens and tight junction mRNAs. In summary, these findings support that chronic stress is associated with increased levels of cytokines and chemokines, their downstream signaling pathways coupled to dysregulation of intestinal cell development and function. Epigenetic regulation of chromatin remodeling likely plays a prominent role in this process. Results also suggest that super enhancers play a primary role in chronic stress-associated intestinal barrier dysfunction.

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Intestinal inflammatory profile shows increase in a diversity of biomarkers in irritable bowel syndrome

Cited by 17 publications

References 44 publications

Open‐label pilot study of ethosuximide as adjunctive therapy for relieving abdominal pain related to Irritable Bowel Syndrome

Open‐label pilot study of ethosuximide as adjunctive therapy for relieving abdominal pain related to Irritable Bowel Syndrome

Disruption of the pro-inflammatory, anti-inflammatory cytokines and tight junction proteins expression, associated with changes of the composition of the gut microbiota in patients with irritable bowel syndrome

Chronic psychological stress alters gene expression in rat colon epithelial cells promoting chromatin remodeling, barrier dysfunction and inflammation

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