Intestinal morphology and microflora to Vibrio alginolyticus in pacific white shrimp (Litopenaeus vannamei)

Liao, Guowei; Wu, Qiuping; Mo, Binhua; Jiang, Zhou; Li, Junyi; Zou, Jixing; Fan, Lanfen

doi:10.1016/j.fsi.2022.01.026

Cited by 16 publications

(5 citation statements)

References 42 publications

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“…It has been proven that the overabundance of certain bacterial community may have impacted the disease structure of the host and trigger the changes in the health status of the gut microbiota (Xiong et al, 2015). Furthermore, Vibrio species, such as V. harveyi, V. parahaemolyticus, V. alginolyticus, V. owensii and V. anguillarum were reported as common pathogenic bacteria in various marine animals (Lee et al, 2015;Chen et al, 2017;Deng et al, 2020;Deris et al, 2020;Zhang et al, 2020;Liao et al, 2022;Muthukrishnan et al, 2022). In fact, an overabundance of Vibrio species was found to be intimately related to the progression of shrimp, lobster, and crab diseases such as AHPND, Vibrio-caused bacteraemia, shell disease, luminescent vibriosis and septic hepatopancreatic necrosis disease (Diggles et al, 2000;Vogan et al, 2002;Jayasree et al, 2006;Nie et al, 2017;Morales-Covarrulbias et al, 2018;Xia et al, 2018;Prachumwat et al, 2020;Nurhafizah et al, 2021;Lioa et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Understanding the effects of salinity and Vibrio harveyi on the gut microbiota profiles of Litopenaeus vannamei

Deris

Iehata

Gan³

et al. 2022

Front. Mar. Sci.

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Increasing evidence have revealed a positive correlation between gut microbiota and shrimp health, in which a healthy shrimp gut consists of a complex and stable microbial community. Given that both abiotic and biotic factors constantly regulate shrimp gut microbiota, any changes affecting the levels of these factors could cause modification to the gut microbiota assemblage. The goal of this study was to explore the effects of salinity levels and pathogenic Vibrio harveyi infection on the diversity, structure, composition, interspecies interaction, and functional pathways of Litopenaeus vannamei gut microbiota. Juvenile shrimp were cultured at 5 ppt, 20 ppt, and 30 ppt for two months prior to Vibrio harveyi infection. After pathogenic V. harveyi challenge test, genomic DNA was isolated from the shrimp gut, and subjected to the 16S rRNA metagenomic sequencing analysis. We observed that gut microbiota diversity of shrimp cultured at 5 ppt and 30 ppt were lower than those cultured at 20 ppt after exposure to V. harveyi infection, suggesting that shrimp cultured at the two former salinity levels were more susceptible to V. harveyi infection. Network analysis also showed that shrimp cultured at 20 ppt exhibit a more stable bacterial network with complex interspecies interaction, even after induced by V. harveyi. Moreover, the presence of a high number of beneficial bacteria such as Pseudoruegeria, Rhodovulum, Ruegeria, Shimia and Lactobacillus in shrimp cultured at 20 ppt might have played a role in inhibiting the growth of V. harveyi and other potentially pathogenic bacteria. Besides, bacterial functional pathway prediction has also shown that metabolic pathways such as phenylalanine metabolism, glycine, serine and threonine metabolism, starch and sucrose metabolism, glyoxylate and dicarboxylate metabolism, carbon metabolism and biofilm formation process were significantly higher in shrimp cultured at 20 ppt. Collectively, our results suggested that 20 ppt is an optimal salinity that suppresses the growth of V. harveyi and potential pathogenic bacteria in the shrimp gut, which could possibly minimize the risk of pathogenic infection for sustainable production of healthy shrimp.

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Section: Discussionmentioning

confidence: 99%

Understanding the effects of salinity and Vibrio harveyi on the gut microbiota profiles of Litopenaeus vannamei

Deris

Iehata

Gan³

et al. 2022

Front. Mar. Sci.

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“…Simultaneously, it has been reported that sediment bacterial communities contribute significantly to regulate sediment quality and maintain pond ecological functions (Zhang et al, 2021b). Notably, some pathogenic bacterial species, such as Vibrio parahaemolyticus, Vibrio alginolyticus, Vibrio splendidus, Aeromonas hydrophila, and Aeromonas caviae, are frequently associated with disease outbreak of shrimp, crab, and shellfish (Hong To et al, 2020;Liao et al, 2022;Zhou et al, 2019). Bacterial community composition and diversity in pond sediment are thereby recognized as potential indicators for pond ecosystem health (Su et al, 2018;Wu et al, 2023).…”

Section: Introductionmentioning

confidence: 99%

Profiling sediment bacterial communities and the response to pattern-driven variations of total nitrogen and phosphorus in long-term polyculture ponds

Zhang,

Li,

et al. 2024

Front. Mar. Sci.

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Sediment bacterial communities are decisive drivers of nutrient cycling processes in aquaculture ecosystems and are readily affected by surrounding environmental factors. However, the knowledge of sediment nutrient accumulations and bacterial community structure is limited in the emerging polyculture systems. Herein, we investigated the profiles of sediment properties and bacterial communities in six typical polyculture ponds and primarily explored the influence of total nitrogen and phosphorus on the bacterial species and diversity. In almost all sediment samples, Proteobacteria, Chloroflexi, and Bacteroides were the dominant species at the phylum level, and the five most abundant bacterial genera were Sulfurovum, Woeseia, Ilumatobacter, Robiginitalea, and Cyanobium_PCC-6307. A clear different bacterial community was observed with the most dominant bacterial phylum Firmicutes and the lowest bacterial diversity in TZ1 pond sediment; meanwhile, the TZ1 pond also showed the highest TN and TP concentrations. Notably, sediments from WZ1 and WZ2 ponds in low-latitude regions exhibited higher bacterial richness and diversity. Based on Pearson’s correlation analysis, bacterial α-diversity indices showed significant negative correlation with sediment TP content, and TN content contributed the most to the abundance of sediment dominant bacterial genus, indicating that the bacterial community is highly associated with sediment nutrient concentrations. Moreover, co-occurrence network analysis further revealed some keystone taxa that exhibited high correlations with other bacterial species, especially the high-abundance genus Robiginitalea bridging a large number of connections. Compared to traditional mono-mariculture pattern, our study provided direct evidence of lower nutrient loadings and different bacterial communities in the polyculture ponds. This could assist polyculture practitioners in developing effective strategies for detailed nutritional management.

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“…The virulence factors of AHPND-causing bacteria cause perturbations in the shrimp microbiome promoting the development of the disease ( Zhang et al, 2021 ). Relevant microbiome studies have identified biomarkers associated with the pathogenic AHPND-causing V. parahaemolyticus ( Restrepo et al, 2021 ), non-AHPND V. parahaemolyticus ( Zhang et al, 2021 ), and V. alginolyticus ( Liao et al, 2022 ) in juvenile P. vannamei shrimp through experimental studies using challenge tests. For example, the genera, Pseudoalteromonas , Bacterioborax , Ruegeria , and other members of the Vibrionales order were the most abundant in juvenile shrimp challenged with a Vibrio strain causing AHPND ( Restrepo et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

“…Oceanospirillum , Lewinella , and Saprospira were the most abundant genera in juvenile shrimp challenged with a non-AHPND V. parahaemolyticus strain ( Zhang et al, 2021 ). Pseudomonas , Candidatus_Bacilloplasma , and Shewanella were the most abundant genera in juvenile shrimp challenged with a strain of V. alginolyticus ( Liao et al, 2022 ). On the other hand, the microbiomes of healthy and juvenile-farmed P. vannamei shrimp naturally affected by AHPND were also compared through observational studies ( Chen et al, 2017 ; Cornejo-Granados et al, 2017 ; Dong et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

Microbial signature profiles of Penaeus vannamei larvae in low-survival hatchery tanks affected by vibriosis

Reyes,

Andrade,

Betancourt

et al. 2023

PeerJ

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Vibriosis is caused by some pathogenic Vibrio and produces significant mortality in Pacific white shrimp Penaeus (Litopenaeus) vannamei larvae in commercial hatcheries. Acute hepatopancreatic necrosis disease (AHPND) is an emerging vibriosis affecting shrimp-producing countries worldwide. Zoea 2 syndrome is another type of vibriosis that affects the early stages of P. vannamei larvae. Although the pathogenesis of AHPND and zoea 2 syndrome is well known, there is scarce information about microbial composition and biomarkers of P.vannamei larvae affected by AHPND, and there is no study of the microbiome of larvae affected by zoea 2 syndrome. In this work, we characterized the microbiome of P. vannamei larvae collected from 12 commercial hatchery tanks by high-throughput sequencing. Seven tanks were affected by AHPND, and five tanks were affected by zoea 2 syndrome. Subsequently, all samples were selected for sequencing of the V3–V4 region of the16S rRNA gene. Similarity analysis using the beta diversity index revealed significant differences in the larval bacterial communities between disease conditions, particularly when Vibrio was analyzed. Linear discriminant analysis with effect size determined specific microbial signatures for AHPND and zoea 2 syndrome. Sneathiella, Cyclobacterium, Haliea, Lewinella, among other genera, were abundant in AHPND-affected larvae. Meanwhile, Vibrio, Spongiimonas, Meridianimaribacter, Tenacibaculum, among other genera, were significantly abundant in larvae affected by zoea 2 syndrome. The bacterial network at the phylum level for larvae collected from tanks affected by AHPND showed greater complexity and connectivity than in samples collected from tanks affected by zoea 2 syndrome. The bacterial connections inter Vibrio genera were higher in larvae from tanks affected by zoea 2 syndrome, also presenting other connections between the genera Vibrio and Catenococcus. The identification of specific biomarkers found in this study could be useful for understanding the microbial dynamics during different types of vibriosis.

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Intestinal morphology and microflora to Vibrio alginolyticus in pacific white shrimp (Litopenaeus vannamei)

Cited by 16 publications

References 42 publications

Understanding the effects of salinity and Vibrio harveyi on the gut microbiota profiles of Litopenaeus vannamei

Understanding the effects of salinity and Vibrio harveyi on the gut microbiota profiles of Litopenaeus vannamei

Profiling sediment bacterial communities and the response to pattern-driven variations of total nitrogen and phosphorus in long-term polyculture ponds

Microbial signature profiles of Penaeus vannamei larvae in low-survival hatchery tanks affected by vibriosis

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