Intestinal sodium/glucose cotransporter 3 expression is epithelial and downregulated in obesity

Involvement of Intestinal Goblet Cells and Changes in Sodium Glucose Transporters Expression: Possible Therapeutic Targets in Autistic BTBR T+Itpr3tf/J Mice

“…In this regard, however, it is important to underline the difference in results compared to those obtained by Sotak et al [ 18 ]; in fact, these authors showed that diabetic BTBR ob/ob mice showed downregulation of Sglt-3 and not upregulation as we have demonstrated. The different results can be explained because it is true that we used the same strain of animals, but in the case of Sotak et al [ 18 ] the animals were diabetic and obese. Instead, our results showed no difference between the groups in relation to body weight.…”

“…For better explaining and focusing our findings, we carried out biological and molecular studies of two sodium/glucose cotransporters. We studied the Sglt-1 protein that is responsible for transporting the monosaccharide from the intestinal lumen to enterocytes [ 55 ] and Sglt-3, which is considered a glucose sensor [ 18 ]. In agreement with the role of these proteins, their expressions were different in BTBR and CTR mice.…”

“…Taking together all the considerations about this disease and links to obesity and GIT alterations, the aim of this work was to study small intestinal samples from an autistic animal model (BTBR T+Itpr3tf/J or BTBR mice) to identify the markers involved in GIT alterations that are linked to being overweight. We used these animals since our starting point was the paper of Sotak et colleagues [ 18 ]. BTBR mice are considered a good ASD-like model that present behavioral and physiological alterations like those observed in patients with ASD.…”

“…In terms of biological evaluation, it is well-known that intestinal absorption of nutrients is more rapid and efficient in obese than in lean humans [ 38 ]; impaired regulation of glucose homeostasis regulated by sodium-glucose transporters, also known as sodium/glucose cotransporters (Sglt-1 and Sglt-3), has been suggested as an important component of obesity and associated comorbidities. The recent evaluation of a homolog of Sglt-1, Sglt-3, found its expression changed in this pathological condition [ 18 ]. Thus, the other aim of this study was to characterize the expression of Sglt-1 and Sglt-3 in BTBR mice to highlight the important role of glucose transporters in ASD.…”

Lipoxins reduce obesity-induced adipose tissue inflammation in 3D-cultured human adipocytes and explant cultures

“…Omental adipose tissue was paraffin embedded, sectioned and stained ( Soták et al., 2021 ). Briefly, deparaffinized sections were antigen retrieved by boiling sections in a pressure cooker (Antigen Retriever 2100) for 20 min in antigen retrieval buffer (0.05% Tween-20, 10 mM Citrate unmasking buffer, pH 6.0 for FPR2 staining and 0.05% Tween-20, 1 mM EDTA, 10 mM Tris, pH 9.0 for CD68/CD206/CD11c staining).…”