Intestinal transcriptomes in Kazakh sheep with different haplotypes after experimental<i>Echinococcus granulosus</i>infection

Li, Xin; Song, Jukun; Wang, Xuhai; Jia, Bin

doi:10.1051/parasite/2021011

Cited by 3 publications

(6 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It's reported that the orally-infected E. granulosus tightly installed in the intestine of dog, thereby establishing strong adhesion to crypts and impairing the intestinal mucus integrity (33). Additionally, the parasite can damage the epithelial tissue barrier, then penetrate and migrate to other organs in the Kazakh sheep (22). In the present study, we observed that intraperitoneally EgPSC infection reduces the colon length and cecum size of mice.…”

Section: Discussionsupporting

confidence: 59%

“…studies have recognized the relationship between intestinal immune-related genes expression and CE (42). For example, Kazakh sheep with E. granulosus infection were found higher expression level of lectins receptors in intestines, facilitating the capability to respond and eliminate orally acquired E. granulosus (22). Here, we found that intraperitoneally EgPSC-infected mice show lower expression level of Stat4 and IL12rb1.…”

Section: Discussionmentioning

confidence: 47%

“…important in host anti-parasitic effect, as CE-resistant sheep exhibited significant higher level of DEGs related to retinol metabolism (22). Our result revealed that non-orally transmitted E. granulosus has an effect on retinol metabolism in the colon tissue.…”

Section: Discussionmentioning

confidence: 57%

“…Recently, growing evidence demonstrates that EgPSC infection can regulate metabolism and influence metabolism-related diseases (22,31). In this study, as shown in Figure 7, key genes associated with cancer cells metabolism (Pfkfb3, Fgfbp1, Gnas, Pfkfb4, Ctnnb1, and Tsta3), glucose metabolism (Pgls and Hkdc1), lipid synthesis (Agpat4, Pklr, and Ctnnb1), lipid hydrolysis (Enpp4), lipid transport (Agap2), fatty acid metabolism (Mcpt1), amino acid metabolism (Arg2 and Glud1), cell differentiation (Tacc1), and tissue repairment (Fgfbp1) were remarkably downregulated in EgPSC-infected mice.…”

Section: Differentially Expressed Genes Involved In Metabolism After ...mentioning

confidence: 99%

“…Moreover, the excretory-secretory products (ESPs) derived from the E. granulosus could induce the differentiation of immunosuppressive cells to downregulate the immunity against parasitic infection (12,13,21). In previous studies, orally infected model was widely employed to investigate E. granulosus-associated intestinal alternations (22,23). However, the impact of E. granulosus infection on intestine during non-oral transmission remains elusive.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

The non-oral infection of larval Echinococcus granulosus induces immune and metabolic reprogramming in the colon of mice

Zhou

Luo²,

Gong³

et al. 2023

Front. Immunol.

View full text Add to dashboard Cite

BackgroundThe intestinal tract serves as a critical regulator for nutrient absorption and overall health. However, its involvement in anti-parasitic infection and immunity has been largely neglected, especially when a parasite is not transmitted orally. The present study investigated the colonic histopathology and functional reprogramming in mice with intraperitoneal infection of the larval Echinococcus granulosus (E. granulosus).ResultsCompared with the control group, the E. granulosus–infected mice exhibited deteriorated secreted mucus, shortened length, decreased expression of tight junction proteins zonula occludens-1 (ZO-1), and occludin in the colon. Moreover, RNA sequencing was employed to characterize colonic gene expression after infection. In total, 3,019 differentially expressed genes (1,346 upregulated and 1,673 downregulated genes) were identified in the colon of infected mice. KEGG pathway and GO enrichment analysis revealed that differentially expressed genes involved in intestinal immune responses, infectious disease-associated pathways, metabolism, or focal adhesion were significantly enriched. Among these, 18 tight junction-relative genes, 44 immune response-associated genes, and 23 metabolic genes were annotated. Furthermore, mebendazole treatment could reverse the colonic histopathology induced by E. granulosus infection.ConclusionsIntraperitoneal infection with E. granulosus induced the pathological changes and functional reprogramming in the colon of mice, and mebendazole administration alleviated above alternations, highlighting the significance of the colon as a protective barrier against parasitic infection. The findings provide a novel perspective on host-parasite interplay and propose intestine as a possible target for treating parasitic diseases that are not transmitted orally.

show abstract

Section: Discussionsupporting

confidence: 59%

Section: Discussionmentioning

confidence: 47%

Section: Discussionmentioning

confidence: 57%

Section: Differentially Expressed Genes Involved In Metabolism After ...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The non-oral infection of larval Echinococcus granulosus induces immune and metabolic reprogramming in the colon of mice

Zhou

Luo²,

Gong³

et al. 2023

Front. Immunol.

View full text Add to dashboard Cite

show abstract

Multi-omics data elucidate parasite-host-microbiota interactions and resistance to Haemonchus contortus in sheep

Niciura,

Cardoso,

Ibelli

et al. 2024

Parasites Vectors

View full text Add to dashboard Cite

Background The integration of molecular data from hosts, parasites, and microbiota can enhance our understanding of the complex biological interactions underlying the resistance of hosts to parasites. Haemonchus contortus, the predominant sheep gastrointestinal parasite species in the tropics, causes significant production and economic losses, which are further compounded by the diminishing efficiency of chemical control owing to anthelmintic resistance. Knowledge of how the host responds to infection and how the parasite, in combination with microbiota, modulates host immunity can guide selection decisions to breed animals with improved parasite resistance. This understanding will help refine management practices and advance the development of new therapeutics for long-term helminth control. Methods Eggs per gram (EPG) of feces were obtained from Morada Nova sheep subjected to two artificial infections with H. contortus and used as a proxy to select animals with high resistance or susceptibility for transcriptome sequencing (RNA-seq) of the abomasum and 50 K single-nucleotide genotyping. Additionally, RNA-seq data for H. contortus were generated, and amplicon sequence variants (ASV) were obtained using polymerase chain reaction amplification and sequencing of bacterial and archaeal 16S ribosomal RNA genes from sheep feces and rumen content. Results The heritability estimate for EPG was 0.12. GAST, GNLY, IL13, MGRN1, FGF14, and RORC genes and transcripts were differentially expressed between resistant and susceptible animals. A genome-wide association study identified regions on chromosomes 2 and 11 that harbor candidate genes for resistance, immune response, body weight, and adaptation. Trans-expression quantitative trait loci were found between significant variants and differentially expressed transcripts. Functional co-expression modules based on sheep genes and ASVs correlated with resistance to H. contortus, showing enrichment in pathways of response to bacteria, immune and inflammatory responses, and hub features of the Christensenellaceae, Bacteroides, and Methanobrevibacter genera; Prevotellaceae family; and Verrucomicrobiota phylum. In H. contortus, some mitochondrial, collagen-, and cuticle-related genes were expressed only in parasites isolated from susceptible sheep. Conclusions The present study identified chromosome regions, genes, transcripts, and pathways involved in the elaborate interactions between the sheep host, its gastrointestinal microbiota, and the H. contortus parasite. These findings will assist in the development of animal selection strategies for parasite resistance and interdisciplinary approaches to control H. contortus infection in sheep. Graphical Abstract

show abstract

Multi-omics elucidated parasite-host-microbiota interactions and resistance to Haemonchus contortus in sheep

Niciura,

Cardoso,

Ibelli

et al. 2023

Preprint

View full text Add to dashboard Cite

Background: The integration of molecular data from hosts, parasites, and microbiota can enhance our understanding of the complex biological interactions underlying parasite resistance. Haemonchus contortus, the predominant sheep parasite species in the tropics, results in significant production and economic losses, which are further compounded by the diminishing efficiency of chemical control measures due to anthelmintic resistance. Knowledge of how the host responds to infection and how the parasite, in combination with microbiota effects, modulates host immunity can guide selection decisions to breed more resistant animals. This understanding can also refine management practices and inform the development of new therapeutics for long-term helminth control. Results: Egg per gram (EPG) counts were obtained in Morada Nova sheep subjected to two artificial infections with H. contortus, and used as a proxy to select animals with high resistance or susceptibility. The GAST, GNLY, IL13, MGRN1, FGF14, and RORC genes and transcripts were differentially expressed between groups based on RNA-seq of the abomasum. From 50K SNP genotyping, EPG heritability estimate was 0.12, and a genome-wide association study (GWAS) identified regions on chromosomes 2 and 11 harboring candidate genes for resistance, immune response, body weight, and adaptation. Trans-eQTLs between significant variants and differentially expressed transcripts were found. Amplicon sequence variants (ASVs) from PCR amplification and sequencing of bacterial and archaeal 16S rRNA genes in sheep feces and rumen generated functional co-expression modules correlated with resistance to H. contortus, showing enrichment in pathways of response to bacterium, immune and inflammatory responses, and hub features of the Christensenellaceae, Bacteroides, and Methanobrevibacter genera, Prevotellaceae family, and Verrucomicrobiota. In RNA-seq of H. contortus, some mitochondrial, collagen- and cuticle-related genes were expressed only in parasites retrieved from susceptible sheep. Conclusions: This study identified chromosome regions, genes, transcripts, and pathways involved in the elaborate interactions between the sheep host, its gastrointestinal microbiota and the H. contortus parasite. These findings can assist with the development of animal selection strategies for parasite resistance and interdisciplinary approaches to control H. contortus in sheep.

show abstract

Intestinal transcriptomes in Kazakh sheep with different haplotypes after experimentalEchinococcus granulosusinfection

Cited by 3 publications

References 48 publications

The non-oral infection of larval Echinococcus granulosus induces immune and metabolic reprogramming in the colon of mice

The non-oral infection of larval Echinococcus granulosus induces immune and metabolic reprogramming in the colon of mice

Multi-omics data elucidate parasite-host-microbiota interactions and resistance to Haemonchus contortus in sheep

Multi-omics elucidated parasite-host-microbiota interactions and resistance to Haemonchus contortus in sheep

Contact Info

Product

Resources

About