Intra-discal vancomycin-loaded PLGA microsphere injection for MRSA discitis: an experimental study

Wang, Fei; Ni, Bin; Zhu, Zhuangchen; Liu, Fucun; Zhu, Yu-Zhao; Liu, Jun

doi:10.1007/s00402-010-1154-8

Cited by 20 publications

(29 citation statements)

References 34 publications

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“…As reported in the literature, PLGA possesses favorable features of biocompatibility, nontoxicity, and ease of creation into sacciform or membrane structures. [ 10 ] In addition, if ester bonds in its chemical structure are disrupted, it can be degraded in vivo to lactic acid and glycolic acid,[ 11 ] which are normal catabolites in the human body. Consequently, PLGA is commonly considered to have excellent biosafety and has been extensively applied in the pharmaceutical, medical engineering, and modern industrial fields.…”

Section: Discussionmentioning

confidence: 99%

Biosafety of the Novel Vancomycin-loaded Bone-like Hydroxyapatite/Poly-amino Acid Bony Scaffold

Cao

Jiang

Yan

et al. 2016

Chinese Medical Journal

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Background:Recently, local sustained-release antibiotics systems have been developed because they can increase local foci of concentrated antibiotics without increasing the plasma concentration, and thereby effectively decrease any systemic toxicity and side effects. A vancomycin-loaded bone-like hydroxyapatite/poly-amino acid (V-BHA/PAA) bony scaffold was successfully fabricated with vancomycin-loaded poly lactic-co-glycolic acid microspheres and BHA/PAA, which was demonstrated to exhibit both porosity and perfect biodegradability. The aim of this study was to systematically evaluate the biosafety of this novel scaffold by conducting toxicity tests in vitro and in vivo.Methods:According to the ISO rules for medical implant biosafety, for in vitro tests, the scaffold was incubated with L929 fibroblasts or rabbit noncoagulant blood, with simultaneous creation of positive control and negative control groups. The growth condition of L929 cells and hemolytic ratio were respectively evaluated after various incubation periods. For in vivo tests, a chronic osteomyelitis model involving the right proximal tibia of New Zealand white rabbits was established. After bacterial identification, the drug-loaded scaffold, drug-unloaded BHA/PAA, and poly (methyl methacrylate) were implanted, and a blank control group was also set up. Subsequently, the in vivo blood drug concentrations were measured, and the kidney and liver functions were evaluated.Results:In the in vitro tests, the cytotoxicity grades of V-BHA/PAA and BHA/PAA-based on the relative growth rate were all below 1. The hemolysis ratios of V-BHA/PAA and BHA/PAA were 2.27% and 1.42%, respectively, both below 5%. In the in vivo tests, the blood concentration of vancomycin after implantation of V-BHA/PAA was measured at far below its toxic concentration (60 mg/L), and the function and histomorphology of the liver and kidney were all normal.Conclusion:According to ISO standards, the V-BHA/PAA scaffold is considered to have sufficient safety for clinical utilization.

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Section: Discussionmentioning

confidence: 99%

Biosafety of the Novel Vancomycin-loaded Bone-like Hydroxyapatite/Poly-amino Acid Bony Scaffold

Cao

Jiang

Yan

et al. 2016

Chinese Medical Journal

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“…In general, the vast majority of peptides encapsulated so far were representatives of either peptide antibiotics, e.g. cyclosporin A, vancomycin, and capreomycin, or peptide hormones, such as analogs of GnRH, somatostatin and thymopentin . Peptides in general, however, are of rather high molar mass and difficult candidates with respect to drug development.…”

Section: Introductionmentioning

confidence: 99%

Novel Insights Into Appropriate Encapsulation Methods for Bioactive Compounds Into Polymers: A Study With Peptides and HDAC Inhibitors

et al. 2013

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The use of different nanoparticles (NPs) for successful encapsulation of bioactive substances is discussed. The inclusion efficiency into liposomes, acetalated dextran (Ac-Dex), and variants of poly[(lactic acid)-co-(glycolic acid)] (PLGA) NPs is analyzed after chemical degradation. Efficient inclusion of SIRT1 inhibitor Ex527 in liposomes, Ac-Dex- and PLGA-NPs is observed for all procedures used. Activity of Ex527 is demonstrated by monitoring the acetylation status of SIRT1-target p53. In contrast, small peptides are only incorporated into acid-terminated PLGA-NPs and marginally into Ac-Dex-NPs. The yield depends on peptide sequence and terminal modifications. Activity is exemplified for angiotensin II using the dynamic mass redistribution technology.

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“…Bolus injections of 250 and 500 mg vancomycin, intraosseously, have been administered successfully without eliciting toxic side-effects [ 92 ] while, in a different study, no significant difference between intraosseous and intravenous administration of morphine sulphate was observed [ 93 ]. Drug delivery dosages can also be a combination of four formulations, diffusing at a defined rate while the reservoir is continuously replenished over six weeks by microsphere formulations, as described by Wang et al [ 94 ]. Successful incorporation of such formulations will eliminate the need for administering multiple dosages, further enhancing the functionality of such implants.…”

Section: Discussionmentioning

confidence: 99%

Titanium-Based Hip Stems with Drug Delivery Functionality through Additive Manufacturing

Bezuidenhout

Dimitrov

Staden

et al. 2015

BioMed Research International

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Postoperative infections are a major concern in patients that receive implants. These infections generally occur in areas with poor blood flow and pathogens do not always respond to antibiotic treatment. With the latest developments in nanotechnology, the incorporation of antibiotics into prosthetic implants may soon become a standard procedure. The success will, however, depend on the ability to control the release of antibiotics at concentrations high enough to prevent the development of antibiotic-resistant strains. Through additive manufacturing, antibiotics can be incorporated into cementless femoral stems to produce prosthetic devices with antimicrobial properties. With the emerging increase in resistance to antibiotics, the incorporation of antimicrobial compounds other than antibiotics, preferably drugs with a broader spectrum of antimicrobial activity, will have to be explored. This review highlights the microorganisms associated with total hip arthroplasty (THA), discusses the advantages and disadvantages of the latest materials used in hip implants, compares different antimicrobial agents that could be incorporated, and addresses novel ideas for future research.

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Intra-discal vancomycin-loaded PLGA microsphere injection for MRSA discitis: an experimental study

Cited by 20 publications

References 34 publications

Biosafety of the Novel Vancomycin-loaded Bone-like Hydroxyapatite/Poly-amino Acid Bony Scaffold

Biosafety of the Novel Vancomycin-loaded Bone-like Hydroxyapatite/Poly-amino Acid Bony Scaffold

Novel Insights Into Appropriate Encapsulation Methods for Bioactive Compounds Into Polymers: A Study With Peptides and HDAC Inhibitors

Titanium-Based Hip Stems with Drug Delivery Functionality through Additive Manufacturing

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