Intracallus and Intercallus Morphological Variability of Rice Doubled Haploids Obtained in In Vitro Androgenesis

Ilyushko, M. V.; Гученко, Светлана Сергеевна; Romashova, M. V.

doi:10.3103/s1068367421010080

Cited by 4 publications

(3 citation statements)

References 24 publications

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“…In order to obtain hundreds and thousands of lines in the culture of anthers in vitro, it is necessary to provide for a large percentage of regeneration of green buds and plants in each hybrid combination [18]. Indicators of regenerative ability vary greatly depending on the hybrid and on the genotype, i.e., even within the same hybrid combination, different plants provide different intensity of callus formation and regeneration of green plants: from very low to very high one.…”

Section: Introductionmentioning

confidence: 99%

Method of Anther Culture In Vitro in the Creation of Rice Dihaploids Resistant to Prolonged Flooding

Калинина¹,

Kostylev²

2023

Preprint

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The evaluation of the possibility of obtaining dihaploids by the method of anther culture in vitro to accelerate selection for resistance to prolonged flooding of seeded rice with water was carried out. The object of research is rice hybrids F2 of the Rice Breeding and Seed Production Laboratory of the “Donskoy” ASC, obtained as a result of interbreeding the best economically valuable varieties with samples bearing genes of resistance to prolonged flooding with water. Basic nutrient medium with an optimal composition of nutrition elements and growth hormones stimulating callo- and morphogenesis were used. For the induction of callus formation, 12604 anthers were planted, according to 26 hybrid combinations represented by 68 plants, as a result, 716 neoplasms were obtained, including 586 non–morphogenic callus, 130 regenerating plants. Cultivation of anthers revealed large genotypic differences in the samples. According to the responsiveness to neoplasms, 60% of the panicles showed a positive result, the rest did not give callus. The most responsive to the formation of callus were hybrid combinations: 5009/2 – 84 pcs., 5010/2 – 94 pcs., 4565/3 – 85 pcs., 4641/2 – 69 pcs. They also showed the ability to morphogenesis. Androgenic plants were obtained by 14 hybrid combinations, their share was 1.03% of the total number of inoculated anthers. 30 green regenerative lines from four rice hybrids were obtained, differing in visual morphological assessment: 5009/2 - 5 pcs., 5010/2 – 5 pcs., 4565/3 – 2 pcs., 4641/2 – 18 pcs. The isolated lines are characterized by good responsiveness in the culture of anthers in vitro, carry genes for resistance to prolonged flooding, and can be used in rice breeding programs using DG technologies.

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Section: Introductionmentioning

confidence: 99%

Method of Anther Culture In Vitro in the Creation of Rice Dihaploids Resistant to Prolonged Flooding

Калинина¹,

Kostylev²

2023

Preprint

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“…Extensive work on this technique is being carried out at the Federal State Budget Scientific Institution "Federal Science Center of Rice" [12][13][14] and the Primorsky Research Institute [15,16]. In order to obtain hundreds and thousands of lines in anther culture in vitro, it is necessary to ensure a large percentage of regeneration of green buds and plants in each hybrid combination [17][18][19]. Indicators of regenerative ability vary greatly depending on the hybrid and genotype, i.e., even within the same hybrid combination; different plants provide different rates of callus formation and regeneration of green plants from very low to very high.…”

Section: Introductionmentioning

confidence: 99%

Creation of Rice Doubled Haploids Resistant to Prolonged Flooding Using Anther Culture

Kostylev,

Kalinina,

Vozhzhova

et al. 2023

Plants

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Flood resistance in rice is very important in weed control, as weeds cannot overcome deep water. At present, there are no released varieties in Russia that would meet these requirements. The creation of such varieties will reduce production costs and pesticide load on the ecosystem. The object of the study was second-generation rice hybrids obtained by crossing the best varieties for economically valuable traits with samples carrying genes for resistance to prolonged flooding with water. To create double rice haploids resistant to prolonged flooding, the anther culture method was used, followed by molecular genetic evaluation of dihaploids for the presence of genes for resistance to prolonged flooding. An estimate of the growth energy under deep flooding was carried out according to our own method. As a result of the cultivation of anthers, 130 androgenic regenerated plants were obtained in 14 hybrid combinations. In terms of responsiveness to neoplasms, 60% of the panicles showed a positive result, while the rest 40% did not demonstrate callus formation. In total, 30 green regenerative lines were obtained from four rice hybrids, differing in visual morphological assessment. Large genotypic differences between the samples were revealed. These lines carry long-term flood resistance genes and can be used in rice breeding programs using dihaploids. As a result of the assessment of the growth energy in a number of obtained samples, the potential for rapid elongation of the first leaves, overcoming a large layer of water and accumulation of vegetative mass, was revealed.

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“…In order to provide hundreds and thousands of lines in the culture of anthers in vitro, it is necessary to obtain a large percentage of regeneration of green buds and plants in each hybrid combination [19]. The indicators of regenerative capacity vary greatly depending on the hybrid and on the genotype, i.e.…”

Section: Introductionmentioning

confidence: 99%

An in vitro anther culture method for creating rice dihaploids resistant to prolonged flooding

Kalinina,

Kostylev

2023

E3S Web of Conf.

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An assessment was made of the possibility of obtaining dihaploids by the method of anther culture in vitro to accelerate breeding for resistance to prolonged flooding of rice with water. The object of the study is F2 rice hybrids of the Federal State Budgetary Scientific Institution, Agrarian Research Center “Donskoy” (ARC “Donskoy”) rice breeding and seed laboratory, obtained by crossing the best varieties in terms of economically valuable traits with samples carrying genes for resistance to prolonged flooding with water. Basic nutrient media with the optimal composition of nutrients and growth hormones that stimulate callus and morphogenesis were used. Cultivation of anthers revealed large genotypic differences between the samples. In terms of responsiveness to neoplasms, 1/3 of the number of plants showed a positive result, the rest did not give calli. The most responsive to the formation of calli were hybrid combinations: 5009/2 – 84 pcs., 5010/2 – 94 pcs., 4565/3 –85 pcs., 4641/2 – 69 pcs. They also showed the ability to morphogenesis. Androgenic plants were obtained from 13 hybrid combinations, their share was 1.03% of the total number of inoculated anthers. 30 green regenerated lines were obtained from four rice hybrids, differing in visual morphological assessment: 5009/2 – 5 pcs., 5010/2 – 5 pcs., 4565/3 – 2 pcs., 4641/2 – 18 pcs. The isolated lines are characterized by good responsiveness in anther culture in vitro, carry genes for resistance to prolonged flooding, and can be used in rice breeding programs using DG technologies.

show abstract

Intracallus and Intercallus Morphological Variability of Rice Doubled Haploids Obtained in In Vitro Androgenesis

Cited by 4 publications

References 24 publications

Method of Anther Culture In Vitro in the Creation of Rice Dihaploids Resistant to Prolonged Flooding

Method of Anther Culture In Vitro in the Creation of Rice Dihaploids Resistant to Prolonged Flooding

Creation of Rice Doubled Haploids Resistant to Prolonged Flooding Using Anther Culture

An in vitro anther culture method for creating rice dihaploids resistant to prolonged flooding

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