Intracellular Accumulation as an Indicator of Cytotoxicity to Screen Hepatotoxic Components of Chelidonium majus L. by LC–MS/MS

Wu, Cuiting; Wang, Xin; Xu, Ming; Li, Youping; Di, Xin

doi:10.3390/molecules24132410

Cited by 17 publications

(11 citation statements)

References 24 publications

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“…The medical and pharmacological literature indicates that the main chemical constituents of greater celandine are alkaloids, principally of the benzylisoquinoline type (e.g., coptisine, chelidonine, sanguinarine, berberine, protopine, and chelerythrine), chelidonic, and caffeic acid derivatives (such as caffeoylmalic acid) (Blumenthal et al, 1998; Wu, Wang, Xu, Liu, & Di, 2019). Its latex has the wart‐removing property, attributed to its proteolytic enzymes (Pelikan, 1997).…”

Section: Discussionmentioning

confidence: 99%

Greater celandine (Chelidonium majus L.) for COVID‐19: A twenty‐case series

Gardin¹,

Braga²

2021

Phytotherapy Research

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Wuhan, China, with a rapid increase in cases worldwide. Until now, among several drugs tested, none demonstrated sufficient efficacy for its etiological treatment.Greater celandine (Chelidonium majus L.) is a well-known medicinal plant, traditionally indicated for digestive disorders and topically to remove warts. This study, performed at private offices in São Paulo and Aracaju (Brazil), describes 20 consecutive COVID-19 outpatients treated with greater celandine and their clinical evolution. The patients, aged 14-71 years (median of 41 years), were treated with Chelidonium majus 10% mother tincture, 20-30 drops three times a day for 3-12 days (median of 5 days). Clinical features were assessed during the treatment and at least until 1 week after its end. These cases were considered mild, as most COVID-19 cases.The symptoms were mainly fever, fatigue, cough, sore throat, coryza, anosmia, ageusia, and headache. Ten patients had comorbidities, such as hypertension, diabetes, and overweight. Complete or almost complete clinical improvement occurred within 1-9 days of treatment (median of 3 days). There were no adverse events. This casuistry, although small, may inspire other researchers to continue investigating Chelidonium majus as a healing treatment for COVID-19.

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Section: Discussionmentioning

confidence: 99%

Greater celandine (Chelidonium majus L.) for COVID‐19: A twenty‐case series

Gardin¹,

Braga²

2021

Phytotherapy Research

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“…with or without cell collection by trypsin digestion or cell scraping to prepare cell lysate. 22 However, to decrease volatilization at room temperature, lysing of cells with organic solvents and further scraping of cells from plates are usually conducted on ice, a procedure that is elaborate and time-consuming. A non-volatile SDS solution (0.1%) has been applied in previous studies for the preparation of cell extracts.…”

Section: Discussionmentioning

confidence: 99%

“…Simple cell lysis with 0.025% SDS solution and further protein precipitation with acetonitrile were applied in the present study. Previous researchers used organic solvents (usually 100% methanol) with or without cell collection by trypsin digestion or cell scraping to prepare cell lysate 22 . However, to decrease volatilization at room temperature, lysing of cells with organic solvents and further scraping of cells from plates are usually conducted on ice, a procedure that is elaborate and time‐consuming.…”

Section: Discussionmentioning

confidence: 99%

Determination of intracellular anlotinib, osimertinib, afatinib and gefitinib accumulations in human brain microvascular endothelial cells by liquid chromatography/tandem mass spectrometry

Zhou

et al. 2020

Rapid Comm Mass Spectrometry

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Rationale Brain metastases are a common complication in patients with non‐small‐cell lung cancer (NSCLC). Anlotinib hydrochloride is a novel multi‐target tyrosine kinase inhibitor (TKI) exhibiting a superior overall response rate for brain metastases from NSCLC. The penetrability of anlotinib and three generations of epidermal growth factor receptor (EGFR) TKIs (osimertinib, afatinib and gefitinib) into brain microvascular endothelial cells (HBMECs) was compared. Methods A sensitive quantification method for the four TKIs was developed using liquid chromatography coupled to tandem mass spectrometry (LC/MS/MS). Anlotinib and the three EGFR TKIs were separated on an ACQUITY BEH C18 column after a direct protein precipitation, and then analyzed using electrospray ionization in positive ion mode. The linearity, accuracy, precision, limit of quantification, specificity and stability were assessed. Results The four analytes could be efficiently quantified in a single run of 3.8 min. The validation parameters of all analytes satisfy the acceptance criteria of bioanalytical method guidelines. The calibration range was 0.2–200 ng mL−1 for anlotinib and gefitinib, 1–500 ng mL−1 for osimertinib and 1–200 ng mL−1 for afatinib. The penetration of anlotinib across HBMECs was comparable with that of afatinib and gefitinib but less than that of osimertinib. Conclusions A sensitive LC/MS/MS method to simultaneously measure anlotinib, osimertinib, afatinib and gefitinib in cell extracts was successfully validated and applied to determine their uptake inside HBMECs, which could pave the way for future research on the role of anlotinib in NSCLC brain metastases.

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“…The concentration of protopine in methanolic extract of Fumaria indica was estimated by high-performance thin-layer chromatography (HPTLC) [ 13 ]. Wu et al identified and quantified the intracellular concentration of protopine in HL-7702 cells by liquid chromatography-tandem mass spectrometry (LC-MS/MS) [ 14 ]. Protopine can also be identified and quantified by GC-MS and TLC/GC-MS methods [ 15 , 16 ].…”

Section: Identification and Quantification Of Protopinementioning

confidence: 99%

Identification and Quantification, Metabolism and Pharmacokinetics, Pharmacological Activities, and Botanical Preparations of Protopine: A Review

et al. 2021

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Through pharmacological activity research, an increasing number of natural products and their derivatives are being recognized for their therapeutic value. In recent years, studies have been conducted on Corydalis yanhusuo W.T. Wang, a valuable medicinal herb listed in the Chinese Pharmacopoeia. Protopine, one of its components, has also become a research hotspot. To illustrate the identification, metabolism, and broad pharmacological activity of protopine and the botanical preparations containing it for further scientific studies and clinical applications, an in-depth and detailed review of protopine is required. We collected data on the identification and quantification, metabolism and pharmacokinetics, pharmacological activities, and botanical preparations of protopine from 1986 to 2021 from the PubMed database using “protopine” as a keyword. It has been shown that protopine as an active ingredient of many botanical preparations can be rapidly screened and quantified by a large number of methods (such as the LC-ESI-MS/MS and the TLC/GC-MS), and the possible metabolic pathways of protopine in vivo have been proposed. In addition, protopine possesses a wide range of pharmacological activities such as anti-inflammatory, anti-platelet aggregation, anti-cancer, analgesic, vasodilatory, anticholinesterase, anti-addictive, anticonvulsant, antipathogenic, antioxidant, hepatoprotective, neuroprotective, and cytotoxic and anti-proliferative activities. In this paper, the identification and quantification, metabolism and pharmacokinetics, pharmacological activities, and botanical preparations of protopine are reviewed in detail to lay a foundation for further scientific research and clinical applications of protopine.

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Intracellular Accumulation as an Indicator of Cytotoxicity to Screen Hepatotoxic Components of Chelidonium majus L. by LC–MS/MS

Cited by 17 publications

References 24 publications

Greater celandine (Chelidonium majus L.) for COVID‐19: A twenty‐case series

Greater celandine (Chelidonium majus L.) for COVID‐19: A twenty‐case series

Determination of intracellular anlotinib, osimertinib, afatinib and gefitinib accumulations in human brain microvascular endothelial cells by liquid chromatography/tandem mass spectrometry

Identification and Quantification, Metabolism and Pharmacokinetics, Pharmacological Activities, and Botanical Preparations of Protopine: A Review

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