Intracellular Ca2+ storage organelles in non-muscle cells: Heterogeneity and functional assignment

Meldolesi, Jacopo; Madeddu, Luisa; Pozzan, Tullio

doi:10.1016/0167-4889(90)90113-r

Cited by 159 publications

(64 citation statements)

References 104 publications

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“…The precise identity of second messenger-responsive intracellular CaZ+ stores in nonmuscle cells has been the foc-,s of considerable attention over the last several years (see Meldolesi et al, 1990). The ryanodine receptor (Ellisman et al, 1990;McPherson and Campbell, 1990), vacuolar Ca2+-ATPases (Gunteski-Hamblin et al, 1988;Lytton and MacLennan, 1988;Kaprielian et al, 1989;Plessers et al, 1991) as well as calsequestrin (Volpe et al, 1990a) or proteins functionally analogous to calsequestrin (Booth and Koch, 1989;Fliegel et al, 1989;Treves et al, 1990) have been identified in nonmuscle cells.…”

mentioning

confidence: 99%

Ca2+ stores in Purkinje neurons: endoplasmic reticulum subcompartments demonstrated by the heterogeneous distribution of the InsP3 receptor, Ca(2+)-ATPase, and calsequestrin

et al. 1992

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The nature of second messenger-responsive intracellular Ca2+ stores in neurons remains open for discussion. Here, we demonstrate the existence in Purkinje cells (PCs) of endoplastic reticulum (ER) subcompartments characterized by an uneven distribution of three proteins involved in Ca2+ storage and release: the inositol 1,4,5-trisphosphate (InsP3) receptor, Ca(2+)-ATPase, and calsequestrin. Ca(2+)-ATPase and the InsP3 receptor have a widespread, although not identical, distribution throughout the ER. Calsequestrin is localized throughout the smooth ER and is particularly concentrated in pleiomorphic vesicles with a moderately electron-dense core, which appear to represent a subcompartment of the smooth ER. In double-labeling experiments many of these vesicles were unlabeled by InsP3 receptor antibodies. These results suggest a key role of the ER as an intracellular Ca2+ store and demonstrate a possible structural basis for distinct intracellular Ca2+ pools regulated by different second messengers.

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confidence: 99%

Ca2+ stores in Purkinje neurons: endoplasmic reticulum subcompartments demonstrated by the heterogeneous distribution of the InsP3 receptor, Ca(2+)-ATPase, and calsequestrin

et al. 1992

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“…There is convincing evidence that intracellular Ca 2+ stores are heterogeneous [6] and in bovine adrenal chromaffin cells two immunologically distinct Ca 2+-ATPases with different subcellular distribution have been reported [16]. Our results suggest that tBuBHQ inhibits Ca 2+ uptake by a fraction (about 80070) of intracellular stores that includes, but is not restricted to, the Ins(1,4,5)P3-sensitive stores.…”

Section: Resultsmentioning

confidence: 48%

“…A feature of many models proposed to account for these actions of Ins(1,4,5)P3 is regulated communication between discrete intracellular Ca 2+ stores, only a fraction of which are sensitive to Ins(1,4,5)P~ [5]. However, neither the biochemical characteristics nor the anatomical identity of the Ins(1,4,5)P3-sensitive Ca 2+ stores are known [6]. Pharmacological tools that distinguish the different stores would clearly be useful.…”

Section: Introductionmentioning

confidence: 99%

2,5‐Di‐(tert‐butyl)‐1,4‐benzohydroquinone mobilizes inositol 1,4,5‐trisphosphate‐sensitive and ‐insensitive Ca²⁺ stores

1990

FEBS Letters

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In permeabilized rat hepatocytes a maximal concentration (25/tM) of 2, mobilized 70% of sequestered Ca 2+ and a half-maximal effect was produced by 1.7/iM tBuBHQ. Inositol 1,4,5-trisphosphate (Ins(l,4,5)P3) stimulated release of about 40% of the intracellular Ca 2+ stores. Combined applications of a range of tBuBHQ concentrations with a maximal concentration of Ins(1,4,5)P 3 demonstrated that tBuBHQ has slight selectivity for the Ca 2÷ transport process of the Ins(1,4,5)P3-sensitive stores. We conclude that the Ins( 1,4,5)P3-sensitive stores are a subset of those sensitive to tBuBHQ and that the latter is therefore unlikely to prove useful as a tool to discriminate Ins(1,4,5)P3-sensitive and -insensitive Ca 2÷ stores though it may provide opportunities to design more selective agents.Inositol 1,4,5-trisphosphate; Intracellular Ca 2÷ store; 2,

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“…Although it has been observed that there is a highest Ca 2+ fluorescence at the perinuclear area in various cell types, most of the authors consider it as the result of dye compartmentalization of the ester loaded indicator into internal organelles [30]. It is widely believed that ester loaded Ca 2+ fluorescent indicators tend to be sequestered into subcellular compartments and distort cytoplasmic free Ca 2+ due to different dye concentrations in different cell regions.…”

Section: Discussionmentioning

confidence: 99%

Visualization of Golgia apparatus as an intracellular calcium store by laser scanning confocal microscope

Cui

Xue

1995

Cell Res

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Using laser scanning confocal microscopy, we have found that the in cells loaded with fluo-3/ AM, highest intracellular Ca 2+ in the perinuclear region is associated with the Golgi apparatus. The spatiotemporal subcellular distribution of Ca 2+ in living human fibroblasts exposing to calcium-free medium in response to agonists has been investigated. PDGF, which releases Ca 2+ from intracellular stores by inositol(1, 4, 5)-trisphosphate pathway, produced a biphasic transient rise in intracellular calcium. The initial rise was resulted from a direct release of calcium from the Golgi apparatus. Calcium could be also released from and reaccumulated into the Golgi apparatus by the stimulation of thapsigargin, an inhibitor of the Ca 2+ transport ATPase of intracellular calcium store. Permeablizing the plasma membrane by 10 μM digitonin resulted in the calcium release from the Golgi apparatus and depletion of the internal calcium store. These results suggest that the Golgi apparatus plays a role in Ca 2+ regulation in signal transduction.

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Intracellular Ca2+ storage organelles in non-muscle cells: Heterogeneity and functional assignment

Cited by 159 publications

References 104 publications

Ca2+ stores in Purkinje neurons: endoplasmic reticulum subcompartments demonstrated by the heterogeneous distribution of the InsP3 receptor, Ca(2+)-ATPase, and calsequestrin

Ca2+ stores in Purkinje neurons: endoplasmic reticulum subcompartments demonstrated by the heterogeneous distribution of the InsP3 receptor, Ca(2+)-ATPase, and calsequestrin

2,5‐Di‐(tert‐butyl)‐1,4‐benzohydroquinone mobilizes inositol 1,4,5‐trisphosphate‐sensitive and ‐insensitive Ca²⁺ stores

Visualization of Golgia apparatus as an intracellular calcium store by laser scanning confocal microscope

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Intracellular Ca2+ storage organelles in non-muscle cells: Heterogeneity and functional assignment

Cited by 159 publications

References 104 publications

Ca2+ stores in Purkinje neurons: endoplasmic reticulum subcompartments demonstrated by the heterogeneous distribution of the InsP3 receptor, Ca(2+)-ATPase, and calsequestrin

Ca2+ stores in Purkinje neurons: endoplasmic reticulum subcompartments demonstrated by the heterogeneous distribution of the InsP3 receptor, Ca(2+)-ATPase, and calsequestrin

2,5‐Di‐(tert‐butyl)‐1,4‐benzohydroquinone mobilizes inositol 1,4,5‐trisphosphate‐sensitive and ‐insensitive Ca2+ stores

Visualization of Golgia apparatus as an intracellular calcium store by laser scanning confocal microscope

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2,5‐Di‐(tert‐butyl)‐1,4‐benzohydroquinone mobilizes inositol 1,4,5‐trisphosphate‐sensitive and ‐insensitive Ca²⁺ stores