Intracellular glutathione status regulates mouse bone marrow monocyte-derived macrophage differentiation and phagocytic activity

Kim, Jin Man; Kim, Hyunsoo; Kwon, Soon Bok; Lee, Soo Young; Chung, Sung‐Chang; Jeong, Daewon; Min, Byung‐Moo

doi:10.1016/j.bbrc.2004.09.220

Cited by 39 publications

(28 citation statements)

References 43 publications

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“…This result is in contrast with those of de Pinto and colleagues (2002), who reported a decrease of GSH content in SNPtreated BY-2 tobacco cells, suggesting a diVerent response between cell culture and roots. Nevertheless, a similar response was previously reported for Wssion yeast and animal cells (Kuo and Abe 1996;Moellering et al 1998;Kim et al 2004), and the present report extends the eVect of NO on GSH synthesis pathway to plants. As for yeast and animals, NO triggered an increase of the endogenous GSH amount above control in M. truncatula roots through the stimulation of GSH synthesis gene transcript accumulation.…”

Section: Discussionsupporting

confidence: 87%

“…As for yeast and animals, NO triggered an increase of the endogenous GSH amount above control in M. truncatula roots through the stimulation of GSH synthesis gene transcript accumulation. Whereas only -ecs gene stimulation was tested for GSH accumulation upon NO treatment in yeast and animals (Kuo and Abe 1996;Kim et al 2004), our results underline a concomitant up-regulation of -ecs and gshs gene transcription in M. truncatula.…”

Section: Discussionmentioning

confidence: 57%

“…Several studies have evidenced that the GSH biosynthetic pathway is stimulated in response to NO in animal cells and yeast (Kuo and Abe 1996;Moellering et al 1998;Kim et al 2004). In these reports, the increase of GSH synthesis was attributed to an enhanced expression of the rate-limiting enzyme -ECS.…”

Section: Introductionmentioning

confidence: 96%

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Glutathione synthesis is regulated by nitric oxide in Medicago truncatula roots

Innocenti

Pucciariello²,

Gleuher³

et al. 2006

Planta

143

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Glutathione (GSH) is one of the main antioxidants in plants. Legumes have the specificity to produce a GSH homolog, homoglutathione (hGSH). We have investigated the regulation of GSH and hGSH synthesis by nitric oxide (NO) in Medicago truncatula roots. Analysis of the expression level of gamma-glutamylcysteine synthetase (gamma-ECS), glutathione synthetase (GSHS) and homoglutathione synthetase (hGSHS) after treatment with sodium nitroprusside (SNP) and nitrosoglutathione (GSNO), two NO-donors, showed that gamma-ecs and gshs genes are up regulated by NO treatment whereas hgshs expression is not. Differential accumulation of GSH was correlated to gene expression in SNP-treated roots. Our results provide the first evidence that GSH synthesis pathway is regulated by NO in plants and that there is a differential regulation between gshs and hgshs in M. truncatula.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 57%

See 1 more Smart Citation

Glutathione synthesis is regulated by nitric oxide in Medicago truncatula roots

Innocenti

Pucciariello²,

Gleuher³

et al. 2006

Planta

143

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“…It has also been demonstrated that high levels of GSH/GSSG ratio are important for osteoblast differentiation and mineralization process (8). Indeed, GSH redox state has been reported to play an important role in the differentiation and phenotype expression of some cell types including osteoblasts and osteoclasts (67). Moreover, GSH and NAC in SaOS-2 cells decrease RANKL/OPG levels, while, increase calcium levels and mineralization process (8).…”

Section: Antioxidants In Bone Remodeling and In Bone Lossmentioning

confidence: 99%

Oxidative stress in bone remodeling: role of antioxidants

Domazetovic

Marcucci

Iantomasi

et al. 2017

ccmbm

568

524

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SummaryROS are highly reactive molecules which consist of a number of diverse chemical species, including radical and non-radical oxygen species. Oxidative stress occurs as a result of an overproduction of ROS not balanced by an adequate level of antioxidants. The natural antioxidants are: thiol compounds among which GSH is the most representative, and non-thiol compounds such as polyphenols, vitamins and also various enzymes. Many diseases have been linked to oxidative stress including bone diseases among which one of the most important is the osteoporosis. The redox state changes are also related to the bone remodeling process which allows the continuous bone regeneration through the coordinated action of bone cells: osteoclasts, osteoblasts and osteocytes. Changes in ROS and/or antioxidant systems seem to be involved in the pathogenesis of bone loss. ROS induce the apoptosis of osteoblasts and osteocytes, and this favours osteoclastogenesis and inhibits the mineralization and osteogenesis. Excessive osteocyte apoptosis correlates with oxidative stress causing an imbalance in favor of osteoclastogenesis which leads to increased turnover of bone remodeling and bone loss. Antioxidants either directly or by counteracting the action of oxidants contribute to activate the differentiation of osteoblasts, mineralization process and the reduction of osteoclast activity. In fact, a marked decrease in plasma antioxidants was found in aged or osteoporotic women. Some evidence shows a link among nutrients, antioxidant intake and bone health. Recent data demonstrate the antioxidant properties of various nutrients and their influence on bone metabolism. Polyphenols and anthocyanins are the most abundant antioxidants in the diet, and nutritional approaches to antioxidant strategies, in animals or selected groups of patients with osteoporosis or inflammatory bone diseases, suggest the antioxidant use in anti-resorptive therapies for the treatment and prevention of bone loss.

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“…Intracellular peroxides and free thiol groups were analyzed with 2´,7´-dichlorofluorescin diacetate (Sigma) and 5,5´-dithiobis-(2-nitrobenzoic acid) (Sigma), as described elsewhere [14].…”

Section: Measurement Of Intracellular Peroxides and Free Thiol Groupsmentioning

confidence: 99%

Osteoclast Precursors Display Dynamic Metabolic Shifts toward Accelerated Glucose Metabolism at an Early Stage of RANKL-Stimulated Osteoclast Differentiation

Kim

Jeong

Kang

et al. 2007

Cell Physiol Biochem

Self Cite

113

116

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Mature osteoclasts have an increased citric acid cycle and mitochondrial respiration to generate high ATP production and ultimately lead to bone resorption. However, changes in metabolic pathways during osteoclast differentiation have not been fully illustrated. We report that glycolysis and oxidative phosphorylation characterized by glucose and oxygen consumption as well as lactate production were increased during receptor activator of nuclear factor-ĸB ligand (RANKL)-induced osteoclastogenesis from RAW264.7 and bone marrow-derived macrophage cells. Cell proliferation and differentiation varied according to glucose concentrations (0 to 100 mM). Maximal cell growth occurred at 20 mM glucose concentration and differentiation occurred at 5 mM concentration. Despite the similar growth rates exhibited when cultured cells were exposed to either 5 mM or 40 mM glucose, their differentiation was markedly decreased in high glucose concentrations. This finding suggests the possibility that osteoclastogenesis could be regulated by changes in metabolic substrate concentrations. To further address the effect of metabolic shift on osteoclastogenesis, we exposed cultured cells to pyruvate, which is capable of promoting mitochondrial respiration. Treatment of pyruvate synergistically increased osteoclastogenesis through the activation of RANKL-stimulated signals (ERK and JNK). We also found that osteoclastogenesis was retarded by blocking ATP production with either the inhibitors of mitochondrial complexes, such as rotenone and antimycin A, or the inhibitor of ATP synthase, oligomycin. Taken together, these results indicate that glucose metabolism during osteoclast differentiation is accelerated and that a metabolic shift towards mitochondrial respiration allows high ATP production and induces enhanced osteoclast differentiation.

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Intracellular glutathione status regulates mouse bone marrow monocyte-derived macrophage differentiation and phagocytic activity

Cited by 39 publications

References 43 publications

Glutathione synthesis is regulated by nitric oxide in Medicago truncatula roots

Glutathione synthesis is regulated by nitric oxide in Medicago truncatula roots

Oxidative stress in bone remodeling: role of antioxidants

Osteoclast Precursors Display Dynamic Metabolic Shifts toward Accelerated Glucose Metabolism at an Early Stage of RANKL-Stimulated Osteoclast Differentiation

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