Intracellular Golgi Complex organization reveals tissue specific polarity during zebrafish embryogenesis

Sepich, Diane S.; Solnica‐Krezel, Lila

doi:10.1002/dvdy.24409

Cited by 13 publications

(10 citation statements)

References 115 publications

(173 reference statements)

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“…Strikingly, during early embryonic development in zebrafish, Golgi markers initially display a dispersed and punctate pattern. Around mid-gastrulation, the Golgi apparatus condenses in some cell types – like in the most superficial epithelial cells of the gastrula – evidently forming a ribbon, while other cell types maintain a dispersed pattern until later in development (Sepich and Solnica-Krezel, 2016). The morphological variation observed shows a potential correlation between more dispersed Golgi elements and a shorter cell cycle.…”

Section: Summary and Perspectivesmentioning

confidence: 99%

A New Look at the Functional Organization of the Golgi Ribbon

Saraste

Prydz

2019

Front. Cell Dev. Biol.

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A characteristic feature of vertebrate cells is a Golgi ribbon consisting of multiple cisternal stacks connected into a single-copy organelle next to the centrosome. Despite numerous studies, the mechanisms that link the stacks together and the functional significance of ribbon formation remain poorly understood. Nevertheless, these questions are of considerable interest, since there is increasing evidence that Golgi fragmentation – the unlinking of the stacks in the ribbon – is intimately connected not only to normal physiological processes, such as cell division and migration, but also to pathological states, including neurodegeneration and cancer. Challenging a commonly held view that ribbon architecture involves the formation of homotypic tubular bridges between the Golgi stacks, we present an alternative model, based on direct interaction between the biosynthetic (pre-Golgi) and endocytic (post-Golgi) membrane networks and their connection with the centrosome. We propose that the central domains of these permanent pre- and post-Golgi networks function together in the biogenesis and maintenance of the more transient Golgi stacks, and thereby establish “linker compartments” that dynamically join the stacks together. This model provides insight into the reversible fragmentation of the Golgi ribbon that takes place in dividing and migrating cells and its regulation along a cell surface – Golgi – centrosome axis. Moreover, it helps to understand transport pathways that either traverse or bypass the Golgi stacks and the positioning of the Golgi apparatus in differentiated neuronal, epithelial, and muscle cells.

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Section: Summary and Perspectivesmentioning

confidence: 99%

A New Look at the Functional Organization of the Golgi Ribbon

Saraste

Prydz

2019

Front. Cell Dev. Biol.

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“…1c) suggesting late endosomal transport to lysosomes or the trans-Golgi network (TGN). Prior to gastrulation the zebrafish Golgi is fragmented, 30 and whereas we could see occasional sfGFP-Vangl2 signal in the proximity of the TGN reporter dsRed-GalT, Vangl2 was predominantly localized elsewhere in the cell (Fig. 1i and Supplementary Fig.…”

Section: Resultsmentioning

confidence: 94%

Live imaging and conditional disruption of native vertebrate planar cell polarity

Jussila¹,

Boswell²,

Ciruna³

2021

Preprint

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Tissue-wide coordination of polarized cytoskeletal organization and cell behaviour, critical for organ morphogenesis and tumour progression, is controlled by asymmetric membrane localization of non-canonical Wnt/planar cell polarity (PCP) signalling components. Understanding the dynamic regulation of PCP thus requires visualization of these core proteins. In vertebrates, immunohistochemical studies have provided snapshots of PCP within tissues while exogenous, fluorescently-tagged reporters have been introduced for live imaging of cell polarity. However, the functionality and spatiotemporal relevance of static and exogenous PCP readouts remain uncertain. Here we fluorescently tag an endogenous core PCP component, Vangl2, in zebrafish. We report on the authentic regulation of vertebrate PCP, through live imaging of this native sfGFP-Vangl2 protein during embryogenesis. We couple sfGFP-Vangl2 with conditional zGrad GFP-nanobody degradation methodologies for tissue-specific interrogation of PCP function. Together, our studies provide crucial insights into the establishment and maintenance of vertebrate PCP and create a powerful experimental paradigm for future investigations.

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“…Rab5, Rab7, Rab11 Stable lines marking Rab proteins in zebrafish to visualize dynamics in early, late, and recycling endosomes, respectively, and endosome biology in vivo (Clark et al, 2011) GalT-EGFP Transgenic tool marking the specific trans-Golgi enzyme galactose-1-phosphate uridyl transferase (GalT) in zebrafish (Sepich and Solnica-Krezel, 2016) Actin and microtubules (MT) cytoskeleton…”

Section: Rab1 Rasmentioning

confidence: 99%

In vivo Functional Genomics for Undiagnosed Patients: The Impact of Small GTPases Signaling Dysregulation at Pan-Embryo Developmental Scale

Lauri

Fasano

Venditti

et al. 2021

Front. Cell Dev. Biol.

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While individually rare, disorders affecting development collectively represent a substantial clinical, psychological, and socioeconomic burden to patients, families, and society. Insights into the molecular mechanisms underlying these disorders are required to speed up diagnosis, improve counseling, and optimize management toward targeted therapies. Genome sequencing is now unveiling previously unexplored genetic variations in undiagnosed patients, which require functional validation and mechanistic understanding, particularly when dealing with novel nosologic entities. Functional perturbations of key regulators acting on signals’ intersections of evolutionarily conserved pathways in these pathological conditions hinder the fine balance between various developmental inputs governing morphogenesis and homeostasis. However, the distinct mechanisms by which these hubs orchestrate pathways to ensure the developmental coordinates are poorly understood. Integrative functional genomics implementing quantitative in vivo models of embryogenesis with subcellular precision in whole organisms contribute to answering these questions. Here, we review the current knowledge on genes and mechanisms critically involved in developmental syndromes and pediatric cancers, revealed by genomic sequencing and in vivo models such as insects, worms and fish. We focus on the monomeric GTPases of the RAS superfamily and their influence on crucial developmental signals and processes. We next discuss the effectiveness of exponentially growing functional assays employing tractable models to identify regulatory crossroads. Unprecedented sophistications are now possible in zebrafish, i.e., genome editing with single-nucleotide precision, nanoimaging, highly resolved recording of multiple small molecules activity, and simultaneous monitoring of brain circuits and complex behavioral response. These assets permit accurate real-time reporting of dynamic small GTPases-controlled processes in entire organisms, owning the potential to tackle rare disease mechanisms.

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Intracellular Golgi Complex organization reveals tissue specific polarity during zebrafish embryogenesis

Cited by 13 publications

References 115 publications

A New Look at the Functional Organization of the Golgi Ribbon

A New Look at the Functional Organization of the Golgi Ribbon

Live imaging and conditional disruption of native vertebrate planar cell polarity

In vivo Functional Genomics for Undiagnosed Patients: The Impact of Small GTPases Signaling Dysregulation at Pan-Embryo Developmental Scale

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