Intracellular imaging and concurrent pH sensing of cancer-derived exosomes using surface-enhanced Raman scattering

Chen, Hui; Luo, Caixia

doi:10.1007/s00216-021-03365-w

Cited by 12 publications

(14 citation statements)

References 50 publications

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“…The 1,087/cm SERS peak intensity of exosomes extracted from the plasma of cancer patients is at least twice that of healthy individuals ( Dong et al, 2020 ). Chen et al (2021b) utilized 4-mercaptobenzoic acid-labeled silver nanoparticles attached to the outer surface of exosomes, where the silver nanoparticles can be used as SERS generators. The Raman reagent 4MBA is susceptible to specific intracellular stimuli and can exhibit different vibrational spectral features accordingly.…”

Section: Sers Technology For Exosome Detectionmentioning

confidence: 99%

“…The Raman reagent 4MBA is susceptible to specific intracellular stimuli and can exhibit different vibrational spectral features accordingly. The use of SERS spectroscopy enables the tracking of intracellular distribution of exosomes and simultaneous quantitative sensing of environmental ph ( Chen et al, 2021b ).…”

Section: Sers Technology For Exosome Detectionmentioning

confidence: 99%

“…In recent years, attempts have been made to use the ultra-high-sensitivity SERS technique for exosome detection in an attempt to develop a new technique for efficient and nondestructive liquid biopsy ( Chen et al, 2021b ; Guerrini et al, 2021 ). Strategies for exosomes detection based on SERS technology can be divided into two types.…”

Section: Introductionmentioning

confidence: 99%

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Advances in the Application of Exosomes Identification Using Surface-Enhanced Raman Spectroscopy for the Early Detection of Cancers

Yang

Jia

2022

Front. Bioeng. Biotechnol.

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Exosomes are small nanoscale vesicles with a double-layered lipid membrane structure secreted by cells, and almost all types of cells can secrete exosomes. Exosomes carry a variety of biologically active contents such as nucleic acids and proteins, and play an important role not only in intercellular information exchange and signal transduction, but also in various pathophysiological processes in the human body. Surface-enhanced Raman Spectroscopy (SERS) uses light to interact with nanostructured materials such as gold and silver to produce a strong surface plasmon resonance effect, which can significantly enhance the Raman signal of molecules adsorbed on the surface of nanostructures to obtain a rich fingerprint of the sample itself or Raman probe molecules with ultra-sensitivity. The unique advantages of SERS, such as non-invasive and high sensitivity, good selectivity, fast analysis speed, and low water interference, make it a promising technology for life science and clinical testing applications. In this paper, we briefly introduce exosomes and the current main detection methods. We also describe the basic principles of SERS and the progress of the application of unlabeled and labeled SERS in exosome detection. This paper also summarizes the value of SERS-based exosome assays for early tumor diagnosis.

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Section: Sers Technology For Exosome Detectionmentioning

confidence: 99%

Section: Sers Technology For Exosome Detectionmentioning

confidence: 99%

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Advances in the Application of Exosomes Identification Using Surface-Enhanced Raman Spectroscopy for the Early Detection of Cancers

Yang

Jia

2022

Front. Bioeng. Biotechnol.

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“…In 2020, Chen et al 60 developed a new sensor that could detect and monitor the behavior of intracellular EVs using hybrid programmable EVs with metal NPs (Fig. 13).…”

Section: Monitoring Biological Behavior With Engineered Evs By Sersmentioning

confidence: 99%

Recent progress in surface-enhanced Raman spectroscopy-based biosensors for the detection of extracellular vesicles

Zheng

Ding

et al. 2022

Anal. Methods

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“…Metal nanoparticles have been widely used in the development and construction of sensor systems and drug carriers due to their excellent biocompatibility, large specific surface area, and remarkable photoelectric properties [1][2][3]. Among them, gold nanoparticles (GNPs) have been frequently employed for drug delivery, sensing, imaging, and photodynamic therapy owing to their high extinction coefficient, distinct optical properties, excellent biocompatibility, and low toxicity [4][5][6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

Detection and imaging of Hg(II) in vivo using glutathione-functionalized gold nanoparticles

Li¹,

Li²,

Wang³

et al. 2022

Beilstein J. Nanotechnol.

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The optical and biological properties of functionalized gold nanoparticles (GNPs) have been widely used in sensing applications. GNPs have a strong binding ability to thiol groups. Furthermore, thiols are used to bind functional molecules, which can then be used, for example, to detect metal ions in solution. Herein, we describe 13 nm GNPs functionalized by glutathione (GSH) and conjugated with a rhodamine 6G derivative (Rh6G2), which can be used to detect Hg(II) in cells. The detection of Hg2+ ions is based on an ion-catalyzed hydrolysis of the spirolactam ring of Rh6G2, leading to a significant change in the fluorescence of GNPs-GSH-Rh6G2 from an “OFF” to an “ON” state. This strategy is an effective tool to detect Hg2+ ions. In cytotoxicity experiments, GNPs-GSH-Rh6G2 could penetrate living cells and detect mercury ions through the fluorescent “ON” form.

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Intracellular imaging and concurrent pH sensing of cancer-derived exosomes using surface-enhanced Raman scattering

Cited by 12 publications

References 50 publications

Advances in the Application of Exosomes Identification Using Surface-Enhanced Raman Spectroscopy for the Early Detection of Cancers

Advances in the Application of Exosomes Identification Using Surface-Enhanced Raman Spectroscopy for the Early Detection of Cancers

Recent progress in surface-enhanced Raman spectroscopy-based biosensors for the detection of extracellular vesicles

Detection and imaging of Hg(II) in vivo using glutathione-functionalized gold nanoparticles

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