Intracellular sensitizer fluorescence: Microfluorimetry and fluorescent spectroscopy

Chernyaeva, Elena B.; Koroteev, N. I.; Litinskaya, L. L.; Pugacheva, N.V.; Vardanyan, A. G.

doi:10.1016/1011-1344(90)85186-z

Cited by 5 publications

(3 citation statements)

References 10 publications

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“…7 and Table 2 indicate that the Hp IX fluorescence co-localizes 50% with that of Rhodamine 123, showing that Hp IX is partially found in mitochondria, which is in agreement with previous work [40]. There is also a 36% overlap of Hp IX with the lysosomal marker, with the remainder probably being distributed in the cytoplasm or other organelles.…”

Section: Cytolocalization In Hela Cellssupporting

confidence: 89%

Relationship between structure and photoactivity of porphyrins derived from protoporphyrin IX

Fernandes

Oliveira

Baptista

2010

J. Porphyrins Phthalocyanines

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Protoporphyrin (Pp IX) derivatives were prepared to study the relationship between photosensitizer structure and photoactivity, with an emphasis on understanding the role of membrane interactions in the efficiency of photosensitizers used in photodynamic therapy (PDT). The synthetic strategies described here aimed at changing protoporphyrin periferic groups, varying overall charge and oil/water partition, while maintaining their photochemical properties. Three synthetic routes were used: (1) modification of Pp IX at positions 31 and 81 by addition of alkyl amine groups of different lengths (compounds 2–5), (2) change of Pp IX at positions 133 and 173, generating alkyl amines (compounds 6 and 7, a phosphate amine (compound 8, and quarternary ammonium compounds (compounds 9 and 10), and (3) amine-alkylation of Hematoporphyrin IX (Hp IX) at positions 31, 81, 133 and 173(compound 12). Strategy 1 leads to hydrophobic compounds with low photocytotoxicity. Strategy 2 leads to compounds 6–10 that have high levels of binding/incorporation in vesicles, mitochondria and cells, which are indicative of high bioavailability. Addition of the phosphate group (compound 8), generates an anionic compound that has low liposome and cell incorporation, plus low photocytotoxicity. Compound 12 has intermediate incorporation and photocytotoxic properties. Compound modification is also associated with changes in their sub-cellular localization: 30% of 8 (anionic) is found in mitochondria as compared to 95% of compound 10 (cationic). Photocytotoxicity was shown to be highly correlated with membrane affinity, which depends on the asymmetrical and amphiphilic characters of sens, as well as with sub-cellular localization.

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Section: Cytolocalization In Hela Cellssupporting

confidence: 89%

Relationship between structure and photoactivity of porphyrins derived from protoporphyrin IX

Fernandes

Oliveira

Baptista

2010

J. Porphyrins Phthalocyanines

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“…The main disadvantage of fluorescence techniques is that the dyes are susceptible to photobleaching which leads to the formation of cytotoxid free radicals and singlet oxygen, and even leads to dissipation of the electrochemical gradient (Chen, 1989). This effect can be used for therapeutic purposes by taking advantage of the different accumulation of certain fluorochromes in different tissues (Chen, 1987(Chen, , 1988Chen et al, 1984;Chernyaeva et al, 1990;Salet and Moreno, 1990;Summerhayes et al, 1982;Zhang et al, 1990). Photobleaching and its deleterious effects can be almost avoided by using low excitation intensities and image acquisition systems (e.g., intensified SIT cameras, or cooled CCD cameras which allow photon integration on the chip).…”

Section: Fluorescence Microscopymentioning

confidence: 99%

Dynamics of mitochondria in living cells: Shape changes, dislocations, fusion, and fission of mitochondria

Bereiter‐Hahn

Vöth

1994

Microscopy Res & Technique

786

496

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Mitochondria are semi-autonomous organelles which are endowed with the ability to change their shape (e.g., by elongation, shortening, branching, buckling, swelling) and their location inside a living cell. In addition they may fuse or divide. These dynamics are discussed. Dislocation of mitochondria may result from their interaction with elements of the cytoskeleton, with microtubules in particular, and from processes intrinsic to the mitochondria themselves. Morphological criteria and differences in the fate of some mitochondria argue for the presence of more than one mitochondrial population in some animal cells. Whether these reflect genetic differences remains obscure. Emphasis is laid on the methods for visualizing mitochondria in cells and following their behaviour. Fluorescence methods provide unique possibilities because of their high resolving power and because some of the mitochondria-specific fluorochromes can be used to reveal the membrane potential. Fusion and fission often occur in short time intervals within the same group of mitochondria. At sites of fusion of two mitochondria material of the inner membrane, the matrix compartment seems to accumulate. The original arrangement of the fusion partners is maintained for some minutes. Fission is a dynamic event which, like fusion, in most cases observed in vertebrate cell cultures is not a straight forward process but rather requires several "trials" until the division finally occurs. Regarding fusion and fission hitherto unpublished phase contrast micrographs, and electron micrographs have been included.

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“…Additionally the kinetics of fluorescence histogram alterations correlated with the FSC histogram alterations completely. May be these data will contribute to understanding of the mechanisms of light-dependent increase of the intracellular accumulation of Hp described previously 6. Our findings show that the subpopulation of cell accumulating CM and other polar pigments with the highest efficiency represents a subpopulation of cells with disturbed membrane permeability.…”

mentioning

confidence: 64%

<title>Kinetic studies of porphyrin distribution in suspensions of tumor cells</title>

Zorin

Мельнов²,

Savitsky³

et al. 1996

SPIE Proceedings

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Using a fluorescence activated cell sorting, we investigated the dynamics of porphyrins in suspensions of tumour cells. In addition to direct studies of the incorporation and output of several porphyrins (hematoporphyrin, hematoporphyrin dimethyl ester, chiorin e6 and its mono-, di-, trimethyl esters) from cells, their transfer between cells was investigated. It was shown that the rate of pigment accumulation by cells correlated with the rate of porphyrin penetration across the plasma membrane. As a result, apolar chiorins and HpDME displayed enhanced staining capacity which was independent on the integrity of plasma membrane of cells. To estimate the rate of pigment redistribution between cells, the suspension of tumour cells loaded with porphyrin had been mixed with unloaded cells and the distribution of all cells according to porphyrin fluorescence was determined in different intervals of time. It was obtained that the highest rate of the pigment transfer between cells was exhibited in the case of moderately apolar pigment. Porphyrins with dominantly hydrophobic and hydrophilic properties had a decreased capacity to intercellular migration. The results of this study indicate that, depending on the photosensitizer used, the processes of its distribution in the bulk of tumour tissue mediated by intercellular exchange may occur with a different rate.

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Intracellular sensitizer fluorescence: Microfluorimetry and fluorescent spectroscopy

Cited by 5 publications

References 10 publications

Relationship between structure and photoactivity of porphyrins derived from protoporphyrin IX

Relationship between structure and photoactivity of porphyrins derived from protoporphyrin IX

Dynamics of mitochondria in living cells: Shape changes, dislocations, fusion, and fission of mitochondria

<title>Kinetic studies of porphyrin distribution in suspensions of tumor cells</title>

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