Intracerebral Peripheral Blood Stem Cell (CD34<sup>+</sup>) Implantation Induces Neuroplasticity by Enhancing β1 Integrin-Mediated Angiogenesis in Chronic Stroke Rats

Shyu, Woei Cherng; Lin, Shinn Zong; Chiang, Ming-Fu; Su, Ching Yuan; Li, Hung

doi:10.1523/jneurosci.5165-05.2006

Cited by 159 publications

(146 citation statements)

References 54 publications

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“…Such a potential has indeed been explored in a related model. Angiogenesis in the ischemic tissues and neuroplasticity are enhanced or improved by implantation of isolated CD34 þ cells (Taguchi et al, 2004;Shyu et al, 2006) or ex vivo expanded endothelial progenitor cells (Kalka et al, 2000;Ohta et al, 2006). Furthermore, enhancing the mobilization of the bone marrow-derived CD34 þ cells could provide another avenue for repairing the injured cerebral tissue.…”

Section: Cd34 + Cells and Trauma Angiogenesis 1341 Discussionmentioning

confidence: 99%

Correlation of CD34+ cells with Tissue Angiogenesis after Traumatic Brain Injury in a Rat Model

Guo¹,

Liu²,

Zhang³

et al. 2009

Journal of Neurotrauma

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Increasing evidence suggests that circulating endothelial progenitor cells, which are a subpopulation of hematopoietic progenitor CD34 þ cells, play a critical role in neovascularization and tissue repair. We have tested the hypothesis that traumatic brain injury (TBI) could mobilize CD34 þ cells to peripheral blood and brain tissue, a process critical for vascular repair, in a rat model of TBI. Male Wistar rats were subjected to controlled fluid percussion. Blood and brain tissue were collected before and after TBI to measure the levels of CD34 þ cells in peripheral blood and to detect their accumulation in the damaged cerebral tissue. Compared with surgery controls, CD34þ cells significantly increased in the peripheral blood and accumulated in the brain tissue of TBI rats. Immunohistochemistry detected new vessels with incomplete CD34þ endothelial-like cell lining and an increased number of microvessels in the injured and surrounding tissue. The results demonstrate a close correlation between an increase in circulating CD34 þ cells in response to traumatic injury and angiogenesis in TBI rat brain. They also suggest that transplantation of CD34 þ cells or augmentation of endogenous CD34 þ cells may be a novel therapeutic approach for patients with TBI.

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Section: Cd34 + Cells and Trauma Angiogenesis 1341 Discussionmentioning

confidence: 99%

Correlation of CD34+ cells with Tissue Angiogenesis after Traumatic Brain Injury in a Rat Model

Guo¹,

Liu²,

Zhang³

et al. 2009

Journal of Neurotrauma

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“…13 Volume of interest indicates the region measured by the three parameters (N-acetylaspartate [NAA], creatine [Cr], and choline and choline-containing compounds [Cho]) in the MRI computer software, which includes both the core and the penumbra regions of the infarcted brain. The volume of interest (3ϫ3ϫ3 mm 3 ) was localized centrally to the infarcted region using 2 or 3 images (transverse and sagittal/coronal).…”

Section: Proton Mr Spectroscopy Assessmentmentioning

confidence: 99%

“…13 Because bis-benzimidelabeled cells showed spontaneous blue fluorescence in their nuclei, cell-type specific antibodies, such as GFAP (1:400; Sigma), MAP-2 (1:200; BM), von Willebrand factor (1:20; Sigma), Nestin (1:400; Sigma), and Neu-N (1:200; Chemicon) conjugated with Cy-3 (Jackson Immunoresearch), were stained to determine whether they colocalized with bis-benzimide in the same cell. The total number of differentiated cells that colocalized with bis-benzimide-labeled cells was measured as previously described.…”

Section: Laser-scanning Confocal Microscopy For Immunofluorescence Comentioning

confidence: 99%

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Efficient Tracking of Non-Iron-Labeled Mesenchymal Stem Cells With Serial MRI in Chronic Stroke Rats

Shyu

Chen

Lin

et al. 2007

Stroke

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Background and Purpose-Although stem cell-based treatments for neurodegenerative diseases have advanced rapidly, there is currently no clinically available method to monitor the fate of transplanted cells in the brain. Methods-To use magnetic resonance imaging for tracking transplanted stem cells in the ischemic rat brain, we used the cellular labeling substance Effectene to transfect a standard contrast agent (Gd-DTPA) into immortalized human bone marrow stromal cells. Results-The transfection efficiency of this method was up to 90%, which is substantially better than pure spontaneous endocytosis or other transfection agents. In addition, cellular uptake of Gd-DTPA in vitro was maintained for Ͼ28 days. Therefore, we could follow transplanted stem cell migration and homing into the penumbric area. Using double immunofluorescence, the transplanted cells were seen to differentiate into glial cells, neurons and vascular endothelial cells. Cortical neurochemical activity as evaluated by proton magnetic resonance spectroscopy ( 1 H-MRS) also increased considerably after immortalized human bone marrow stromal cell transplantation. Conclusion-This method of tracking immortalized human bone marrow stromal cells is highly efficient and allows for nontoxic labeling of cells.

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“…8 Recently, it was shown that intracerebral HSC implantation induced neuroplasticity and improved neurological function in rats after chronic cerebral ischemia. 9 Therefore, central nervous system regeneration by transdifferentiation of HSC might be relevant for diagnosis and therapy of neurodegenerative diseases. This study was undertaken to evaluate the frequency of HSC in the blood of patients with early AD.…”

Section: Introductionmentioning

confidence: 99%

Decreased circulating CD34+ stem cells in early Alzheimer's disease: evidence for a deficient hematopoietic brain support?

et al. 2006

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Hematopoietic stem cells contribute to mammalian brain tissue regeneration by transdifferentiation processes. We found decreased counts of circulating CD34 þ cells in early Alzheimer's dementia (AD; P = 0.01), which significantly correlated with age (r = À0.661; P = 0.001), cerebrospinal fluid b-amyloid (Ab)1-42 (r = À0.467; P = 0.025) and most pronounced the Ab42/40 ratio (r = À0.688; P = 0.005). Our data suggest a deficient regenerative hematopoietic support for the central nervous system in early AD.

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Intracerebral Peripheral Blood Stem Cell (CD34⁺) Implantation Induces Neuroplasticity by Enhancing β1 Integrin-Mediated Angiogenesis in Chronic Stroke Rats

Cited by 159 publications

References 54 publications

Correlation of CD34+ cells with Tissue Angiogenesis after Traumatic Brain Injury in a Rat Model

Correlation of CD34+ cells with Tissue Angiogenesis after Traumatic Brain Injury in a Rat Model

Efficient Tracking of Non-Iron-Labeled Mesenchymal Stem Cells With Serial MRI in Chronic Stroke Rats

Decreased circulating CD34+ stem cells in early Alzheimer's disease: evidence for a deficient hematopoietic brain support?

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Intracerebral Peripheral Blood Stem Cell (CD34+) Implantation Induces Neuroplasticity by Enhancing β1 Integrin-Mediated Angiogenesis in Chronic Stroke Rats

Cited by 159 publications

References 54 publications

Correlation of CD34+ cells with Tissue Angiogenesis after Traumatic Brain Injury in a Rat Model

Correlation of CD34+ cells with Tissue Angiogenesis after Traumatic Brain Injury in a Rat Model

Efficient Tracking of Non-Iron-Labeled Mesenchymal Stem Cells With Serial MRI in Chronic Stroke Rats

Decreased circulating CD34+ stem cells in early Alzheimer's disease: evidence for a deficient hematopoietic brain support?

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Intracerebral Peripheral Blood Stem Cell (CD34⁺) Implantation Induces Neuroplasticity by Enhancing β1 Integrin-Mediated Angiogenesis in Chronic Stroke Rats