Intracytoplasmic sperm injection improving embryo quality: Comparison of the sibling oocytes of Non-Male-Factor couples

Yang, Dunsong; Shahata, M. A. M.; Albader, M.; Al-Natsha, S.; Al-Flamerzia, Mariam; Al-Shawaf, Talha

doi:10.1007/bf02070151

Cited by 44 publications

(37 citation statements)

References 7 publications

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“…Similar results were demonstrated by Hsu et al [6] and Frattarelli et al [7]. Others, however, found embryo quality to be comparable between the two methods [3,[8][9][10][11][12][13], or even superior in ICSI [2,14,15].…”

Section: Introductionsupporting

confidence: 83%

“…These findings indicate that ICSI does not improve or diminish the formation of good embryos; rather, the difference between IVF and ICSI in good embryoformation per oocyte was related to the better fertilization rate obtained with ICSI [15]. Yang et al [14] found that more grade A embryos were obtained by ICSI than by conventional IVF performed on sibling oocytes. However, their study group was comprised of only 13 couples.…”

Section: Discussionmentioning

confidence: 96%

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Comparison of embryo quality between intracytoplasmic sperm injection and in vitro fertilization in sibling oocytes

Yoeli

Orvieto

Ashkenazi

et al. 2008

J Assist Reprod Genet

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Background The aim of the present study was to compare the quality of embryos derived from sibling oocytes by in vitro fertilization (IVF) or ICSI. Methods Consecutive patients with a less than 40% fertilization rate in a previous standard IVF cycle or with relative male factor infertility were recruited for the study. The oocytes retrieved from each patient were divided into two groups for either conventional insemination (group A) or ICSI (group B). Power analysis showed that to detect a 25% difference in the rate of high-quality embryos between the groups with a power of 0.8, at least 1,200 oocytes were needed in each group. Results One hundred seventy-seven patients were included in the study. Group A was comprised of 1,526 oocytes and group B of 1,480 sibling oocytes. As expected, the fertilization rate was significantly higher in group B than group A (67.1 vs. 43.6%, p < 0.001). No significant between-group differences were observed in cleavage rate (92.7 and 89.7%, respectively) and the rate of either grade A embryos (22.6 and 23.9%, respectively) or grade A 1 embryos (37.3 and 33.5%, respectively).However, in the subgroup of patients with relative malefactor infertility (n=36), the rate of grade A 1 embryos was significantly higher in the IVF than the ICSI group (46.4 vs. 29.0%, respectively, p=0.02). Conclusions Embryo quality does not seem to be influenced by the mode of fertilization (IVF or ICSI). We assume that embryo quality depends on intrinsic factors of the gametes involved rather than on the fertilization process per se.

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Section: Introductionsupporting

confidence: 83%

Section: Discussionmentioning

confidence: 96%

Comparison of embryo quality between intracytoplasmic sperm injection and in vitro fertilization in sibling oocytes

Yoeli

Orvieto

Ashkenazi

et al. 2008

J Assist Reprod Genet

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show abstract

“…Some recent reports have shown that ICSI (one egg, one spermatozoon) can be used successfully on non-male factor couples to provide comparable or higher fertilization rates (Palermo et al, 1996;Khamsi et al, 2001) and superior embryo quality (Yang et al, 1996) than conventional IVF. Thus, ICSI has been increasingly used in cases in which conventional IVF would not necessarily be inefficient, especially when there are few oocytes available.…”

Section: Discussionmentioning

confidence: 99%

Should few retrieved oocytes be as an indication for intracytoplasmic sperm injection?

Zhu

et al. 2012

J. Zhejiang Univ. Sci. B

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Abstract:Objective: To reevaluate whether relatively few oocytes obtained in one cycle are an indication for intracytoplasmic sperm injection (ICSI). Methods: A total of 406 cycles with three or fewer retrieved oocytes performed in 396 non-male infertile couples were retrospectively reviewed. Cycles were classified into three groups by different fertilization techniques: the in vitro fertilization (IVF) group, insemination with conventional IVF; the ICSI group, insemination with ICSI though semen parameters were normal; and the rescue ICSI group, re-insemination with ICSI after conventional IVF failure. Results: The ICSI group resulted in higher normal fertilization compared with the conventional IVF group. Correspondingly, the cycle cancellation rate was decreased in the ICSI group, though it was not statistically significant. The clinical pregnancy rate and implantation rate were lower in the ICSI group compared with the conventional IVF group. Rescue ICSI was a method to avert total fertilization failure in conventional IVF, increasing fertilization and ensuring embryo availability for transfer, but the normal fertilization was the lowest due to delayed insemination and the chance of pregnancy was very little. Conclusions: Obtaining only few oocytes in one cycle is not considered as an indication for ICSI when the sperm sample is apparently normal. Rescue ICSI is either not recommended if conventional insemination fails. Such patients should not be subjected to the unnecessary costs and potential risks of ICSI.

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“…Furthermore, when only patients with three or more supernumerary embryos were assessed in either group, the mean cell number and quality were not different between the supernumerary IVF and ICSI embryos. It has been suggested that ICSI produces a significantly higher proportion of morphologically superior embryos than conventional ones (Yang et al, 1996) and high insemination concentra-tion IVF (Oehninger et al, 1996). In contrast, our results show that, whereas the mean cell number of day-2 supernumerary embryos did not differ, the quality of those embryos was better in IVF cycles than in ICSI cycles.…”

Section: Discussionmentioning

confidence: 99%

Blastocyst development from supernumerary embryos after intracytoplasmic sperm injection: a paternal influence?

Shoukir¹,

Chardonnens²,

Campana³

et al. 1998

Human Reproduction

190

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The success of intracytoplasmic sperm injection (ICSI) warrants further study on the role of paternal factors in early human embryogenesis. To investigate whether poor sperm parameters can influence embryo development, we examined the development of ICSI-fertilized embryos to the blastocyst stage. We present results of blastocyst development from supernumerary ICSI embryos after co-culture on monkey kidney epithelial cells. In addition, we compare the development of supernumerary embryos to the blastocyst stage after ICSI and in-vitro fertilization (IVF). Of 168 supernumerary ICSI embryos, 45 (26.8%) developed to blastocysts. Sperm concentration and morphology did not influence blastocyst development. In contrast, blastocysts arose from spermatozoa that had a significantly higher (P = 0.015) forward progressive motility compared with spermatozoa from those patients who failed to produce blastocysts (42.7% versus 28.2%, respectively). Overall the rate of embryo development to the blastocyst stage after ICSI was lower (26.8%) than that after IVF (47.3%). When the rate of blastocyst development was calculated for patients with three or more supernumerary embryos, it remained significantly higher for the IVF patients than for the ICSI patients (45.6% versus 30.0%). There was no significant difference in the mean cell number and quality of the supernumerary embryos between the IVF and ICSI patients. This study confirms previous reports that have postulated that abnormal spermatozoa may manifest a negative paternal effect on preimplantation embryo development.

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Intracytoplasmic sperm injection improving embryo quality: Comparison of the sibling oocytes of Non-Male-Factor couples

Cited by 44 publications

References 7 publications

Comparison of embryo quality between intracytoplasmic sperm injection and in vitro fertilization in sibling oocytes

Comparison of embryo quality between intracytoplasmic sperm injection and in vitro fertilization in sibling oocytes

Should few retrieved oocytes be as an indication for intracytoplasmic sperm injection?

Blastocyst development from supernumerary embryos after intracytoplasmic sperm injection: a paternal influence?

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