Intraepithelial and lamina propria leucocyte subsets in inflammatory bowel disease: an immunohistochemical study of colon and rectal biopsy specimens.

Caballero, T.; Nogueras, F.; Medina, Mutiara; Caracuel, M D; Sola, Chrystelle; Martínez-Salmerón, F J; Rodrigo, María Aparicio; Moral, Raimundo García del

doi:10.1136/jcp.48.8.743

Cited by 14 publications

(15 citation statements)

References 27 publications

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“…In agreement with the reported increase in CD31 CD81 IEL in the rectal mucosa of Crohn's disease patients (30) and enhanced cytolytic activity of these cells (31), IL10(2/2) mice treated with the control diet had the highest proportion of CD31 IEL, most of them also expressing the CD8 phenotype. This was associated with a high incidence of colitis.…”

Section: Discussionsupporting

confidence: 86%

Partial Replacement of Dietary (n-6) Fatty Acids with Medium-Chain Triglycerides Decreases the Incidence of Spontaneous Colitis in Interleukin-10–Deficient Mice

Mañé¹,

Pedrosa²,

Lorén³

et al. 2009

The Journal of Nutrition

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Enteral nutrition has a primary therapeutic effect in active Crohn's disease. It is unknown which nutrient(s) account for this action, but a role for both the amount and type of dietary fat has been postulated. Some clinical and experimental data suggest that medium-chain triglycerides (MCT) may reduce intestinal inflammation. We aimed to assess the effect of replacing part of the dietary fat with MCT on the incidence and severity of colitis in interleukin (IL)-10(-/-) mice under specific pathogen-free conditions. Twenty-four IL-10(-/-) 4-wk-old mice were randomized to receive a control diet based on sunflower oil [(n-6) fatty acids (FA)] and an experimental isocaloric, isonitrogenous diet with 50% sunflower and 50% coconut oil (MCT diet). When the mice were 12 wk old, they were killed and the colon was examined for the presence of colitis, lymphocyte subpopulations and apoptosis, ex vivo cytokine production in supernatant of colon explants, toll-like receptor (TLR)-2 and TLR-9 mRNA, and FA profile in colonic tissue homogenates. Colitis incidence was lower in the IL-10(-/-) mice fed the MCT diet (1/12) than in the mice fed the control diet (8/12; P = 0.03). The histological damage score was also lower in the former (P < 0.0005). Feeding the MCT diet resulted in fewer total and apoptotic intraepithelial CD3+ and lamina propria CD3+CD4+ lymphocytes, as well as downregulated production of IL-6 and interferon-gamma, and reduced TLR-9 mRNA. We conclude that partial replacement of dietary (n-6) FA with MCT decreases the incidence of colitis in a model of spontaneous intestinal inflammation and provide experimental arguments for a possible primary therapeutic effect of MCT in human Crohn's disease.

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Section: Discussionsupporting

confidence: 86%

Partial Replacement of Dietary (n-6) Fatty Acids with Medium-Chain Triglycerides Decreases the Incidence of Spontaneous Colitis in Interleukin-10–Deficient Mice

Mañé¹,

Pedrosa²,

Lorén³

et al. 2009

The Journal of Nutrition

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“…IEL from both patients with Crohn's disease and control patients consisted predominantly of CD3 + T cells and were devoid of B cells. The lack of B cells also excluded a potential contamination of the isolated cell population with lamina propria lymphocytes [14,22,23]. However, it has to be noted that there was a substantial percentage of non-lymphoid contaminating cells within the analyzed cell population.…”

Section: Iel But Not Pbls Display Increased Spontaneous Cytolytic Actmentioning

confidence: 99%

Enhanced cytolytic activity of intestinal intraepithelial lymphocytes in patients with Crohn's disease

Nüssler¹,

Stange²,

Hoffman

et al. 2000

Langenbeck's Archives of Surgery

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“…In patients with IBD, immune cell infiltrates are increased in the intestinal mucosa (5,21), and plasma cell and T-lymphocyte numbers are increased in the lamina propria (21,39). Lymphocytes and macrophages secrete Ucn1, and specific immune cells express its receptor, CRF 2 , after activation by lipopolysaccharides and glucocorticoids (34).…”

mentioning

confidence: 99%

Sex- and corticotropin-releasing factor receptor 2- dependent actions of urocortin 1 during inflammation

Hasdemir

Mhaske

Paruthiyil

et al. 2016

American Journal of Physiology-Regulatory, Integrative and Comp

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Intraepithelial and lamina propria leucocyte subsets in inflammatory bowel disease: an immunohistochemical study of colon and rectal biopsy specimens.

Cited by 14 publications

References 27 publications

Partial Replacement of Dietary (n-6) Fatty Acids with Medium-Chain Triglycerides Decreases the Incidence of Spontaneous Colitis in Interleukin-10–Deficient Mice

Partial Replacement of Dietary (n-6) Fatty Acids with Medium-Chain Triglycerides Decreases the Incidence of Spontaneous Colitis in Interleukin-10–Deficient Mice

Enhanced cytolytic activity of intestinal intraepithelial lymphocytes in patients with Crohn's disease

Sex- and corticotropin-releasing factor receptor 2- dependent actions of urocortin 1 during inflammation

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